A5028777317- Bacterial Identification and Susceptibility Testing
- Antimicrobial Resistance in Staphylococcus
- Antibiotic Resistance in Bacteria
- Clostridium difficile and Clostridium perfringens research
- Streptococcal Infections and Treatments
- Gut microbiota and health
- Mycobacterium research and diagnosis
- Bacterial biofilms and quorum sensing
- Nosocomial Infections in ICU
- Molecular Biology Techniques and Applications
- Medical Device Sterilization and Disinfection
- Cystic Fibrosis Research Advances
- Inhalation and Respiratory Drug Delivery
- Vibrio bacteria research studies
- Legionella and Acanthamoeba research
- Enterobacteriaceae and Cronobacter Research
Icahn School of Medicine at Mount Sinai
2016-2023
Infections caused by antimicrobial resistant bacteria are of increasing concern, especially those due to carbapenem-resistant Enterobacteriaceae pathogens. Included in this group species the Enterobacter cloacae complex, regarding which there is a paucity knowledge on infection biology pathogens, despite their clinical relevance.
ABSTRACT Whole-genome sequencing was used to examine a persistent Enterococcus faecium bacteremia that acquired heteroresistance three antibiotics in response prolonged multidrug therapy. A comparison of the complete genomes before and after each change revealed emergence known resistance determinants for vancomycin linezolid suggested novel mutation fabF , encoding fatty acid synthase, responsible daptomycin nonsusceptibility. Plasmid recombination contributed progressive loss withdrawal drug.
Whole-genome sequencing (WGS) of Staphylococcus aureus is increasingly used as part infection prevention practices. In this study, we established a long-read technology-based WGS screening program all first-episode methicillin-resistant (MRSA) blood infections at major urban hospital. A survey 132 MRSA genomes assembled from long reads enabled detailed characterization an outbreak lasting several months CC5/ST105/USA100 clone among 18 infants in neonatal intensive care unit (NICU). Available...
Abstract Background Whole-genome sequencing (WGS) is increasingly used to map the spread of bacterial and viral pathogens in nosocomial settings. A limiting factor for more widespread adoption WGS hospital infection prevention practices availability standardized tools genomic epidemiology. Methods We developed Pathogen Sequencing Phylogenomic Outbreak Toolkit (PathoSPOT) automate integration medical record data rapid detection tracing outbreaks. To demonstrate its capabilities, we applied...
Methicillin-resistant Staphylococcus aureus (MRSA) causes life-threatening infections in both community and hospital settings is a leading cause of health care-associated (HAIs). We sought to describe the molecular epidemiological landscape patients with MRSA bloodstream (BSIs) at an urban medical center by evaluating clinical characteristics associated two dominant endemic clones.Comprehensive data from electronic records 227 hospitalized ≥18 years old BSI over 33-month period New York City...
Pseudomonas aeruginosa is an opportunistic pathogen that rarely causes pneumonia in otherwise healthy patients. We describe a case of community-acquired P. previously individual who likely acquired the infection from home humidifier.
Abstract Whole-genome sequencing (WGS) of Staphylococcus aureus is increasingly used as part infection prevention practices, but most applications are focused on conserved core genomic regions due to limitations short-read technologies. In this study we established a long-read technology-based WGS screening program all first-episode MRSA blood infections at major urban hospital. A survey 132 genomes assembled from long reads revealed widespread gain/loss accessory mobile genetic elements...
Abstract Clostridioides difficile is a leading cause of health care-associated infections. Although significant progress has been made in the understanding its genome, epigenome C. and functional impact not systematically explored. Here, we performed first comprehensive DNA methylome analysis using 36 human isolates observed great epigenomic diversity. We discovered an orphan methyltransferase with well-defined specificity whose corresponding gene highly conserved across our dataset all ~300...
Abstract Background Methicillin-resistant Staphylococcus aureus (MRSA) causes life-threatening infections in both community and hospital settings is a leading cause of healthcare-associated (HAIs). We sought to describe the molecular epidemiological landscape patients with MRSA bloodstream (BSIs) at an urban medical center by evaluating clinical characteristics associated two dominant endemic clones. Methods Comprehensive data extraction from electronic health records 227 hospitalized ≥18...
Abstract Whole genome sequencing (WGS) is increasingly used to map the spread of bacterial and viral pathogens in nosocomial settings. A limiting factor for more widespread adoption WGS hospital infection prevention practices availability standardized tools genomic epidemiology. Here we present Pathogen Sequencing Phylogenomic Outbreak Toolkit (PathoSPOT), which automates integration medical record data rapid detection tracing outbreaks. To demonstrate its capabilities applied PathoSPOT...
Abstract Background Multidrug-resistant Enterococci are a major cause of nosocomial infections, yet our understanding how resistance emerges during antibiotic treatment remains incomplete. We performed whole- and complete-genome sequencing all paired isolates from 11 Enterococcus faecium 10 faecalis cases that acquired hospitalization at Mount Sinai Hospital. Comparative phylogenetic genomic analyses identified novel mechanisms heteroresistance. Methods 2.5 years electronic health records...