A5079673052- Bacterial Identification and Susceptibility Testing
- Antimicrobial Resistance in Staphylococcus
- Streptococcal Infections and Treatments
- Advanced biosensing and bioanalysis techniques
- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Mycobacterium research and diagnosis
- Bacteriophages and microbial interactions
- Clostridium difficile and Clostridium perfringens research
- Antibiotic Resistance in Bacteria
- Infective Endocarditis Diagnosis and Management
- Parasites and Host Interactions
- Biosensors and Analytical Detection
- Nosocomial Infections in ICU
- Nail Diseases and Treatments
- Neonatal and Maternal Infections
- Gut microbiota and health
- RNA and protein synthesis mechanisms
- Cystic Fibrosis Research Advances
- Bacterial biofilms and quorum sensing
- Heavy Metal Exposure and Toxicity
- Neutropenia and Cancer Infections
- Viral gastroenteritis research and epidemiology
- Enterobacteriaceae and Cronobacter Research
- Burkholderia infections and melioidosis
Icahn School of Medicine at Mount Sinai
2015-2019
Mount Sinai Medical Center
2019
Mount Sinai Health System
2016-2018
Mount Sinai Hospital
2015-2018
Mount Sinai Hospital
2018
Rush University Medical Center
2018
Columbia College - Missouri
2018
Mount Sinai Hospital
2017
University of Alberta
2005-2015
University of Utah
2014-2015
Candida auris, an emerging fungus that can cause invasive infections, is associated with high mortality and often resistant to multiple antifungal drugs. C. auris was first described in 2009 after being isolated from external ear canal discharge of a patient Japan (1). Since then, reports including bloodstream have been published several countries, Colombia, India, Israel, Kenya, Kuwait, Pakistan, South Africa, Korea, Venezuela, the United Kingdom (2-7). To determine whether present States...
Although the New York/New Jersey (NY/NJ) area is an epicenter for carbapenem-resistant Enterobacteriaceae (CRE), there are few multicenter studies of CRE from this region. We characterized patients with bacteremia in 2013 at eight NY/NJ medical centers and determined prevalence carbapenem resistance among bloodstream isolates mechanisms, genetic backgrounds, capsular types (cps), antimicrobial susceptibilities. Of 121 bacteremia, 50% had cancer or undergone transplantation. The prevalences...
Single-stranded DNA or RNA aptamer molecules have usually been selected against purified target molecules. To eliminate the need of purifying on cell surface, we developed a selection technique using live bacterial cells in suspension as targets, to select for ssDNA aptamers specific surface Lactobacillus acidophilus were chosen demonstrate proof principle based their high abundance (potential targets). Aptamer pools obtained after 6−8 rounds demonstrated affinity and selective binding with...
This paper describes the selection of high affinity DNA aptamers binding to multiple M-types pathogenic species Streptococcus pyogenes (Group A or GAS). Unlike common aptamer techniques that use purified molecules a monoclonal cell population as targets, this work has achieved against various S. pyogenes. Cell mixtures containing equal numbers 10 most prevalent were incubated with 80-nucleotide libraries, centrifuged, and washed separate cell-bound from unbound sequences. The bound cells was...
The goal of this study is to assess the ability T2Candida platform (T2 Biosystems, Lexington, MA) accurately identify Candida species from pediatric blood specimens with low volumes.Whole 15 children candidemia was collected immediately following culture draw. amount required by system reduced pipetting whole directly onto cartridge. Specimens were subsequently run on T2Dx Instrument Biosystems).The panel provided appropriate result for each specimen compared culture-based identification and...
ABSTRACT Whole-genome sequencing was used to examine a persistent Enterococcus faecium bacteremia that acquired heteroresistance three antibiotics in response prolonged multidrug therapy. A comparison of the complete genomes before and after each change revealed emergence known resistance determinants for vancomycin linezolid suggested novel mutation fabF , encoding fatty acid synthase, responsible daptomycin nonsusceptibility. Plasmid recombination contributed progressive loss withdrawal drug.
From 2015 to 2017, 11 confirmed brucellosis cases were reported in New York City, leading 10 Brucella exposure risk events ( events) 7 clinical laboratories (CLs). Most patients had traveled countries where is endemic and presented with histories findings consistent brucellosis.
In a liver transplant recipient with vancomycin-resistant Enterococcus (VRE) surgical site and bloodstream infection, combination of pulsed-field gel electrophoresis, multilocus sequence typing, whole genome sequencing identified that donor VRE isolates were highly similar when compared to time-matched hospital isolates. Comparison de novo assembled isolate genomes was suggestive transmission rather than hospital-acquired also subtle internal rearrangements between missed by other genomic...
ABSTRACT Whole-genome sequences for Stenotrophomonas maltophilia serial isolates from a bacteremic patient before and after development of levofloxacin resistance were assembled de novo differed by one single-nucleotide variant in smeT , repressor multidrug efflux operon smeDEF . Along with sequenced five contemporaneous cases, they displayed considerable diversity compared against all published complete genomes. sequencing assembly can conclusively identify mechanisms emerging S. strains...
Antimicrobial resistance has emerged as a major threat to global public health. Thus, the surveillance of changes in antimicrobial local and settings is paramount necessity. While many studies have tracked resistance, only small percentage surveyed ocular isolates. The purpose this study was report vitro susceptibility bacterial pathogens isolated from samples New York, NY 2010 2015.
We report the first documented isolation of Wohlfahrtiimonas chitiniclastica from a human in United States. Initially misidentified as Acinetobacter lwoffii by Vitek-2, isolate was subsequently identified W. matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequencing. While clinical significance this case is unclear, it highlights superior performance MALDI-TOF MS for bacterial identification.
Abstract Introduction Pre‐transplant screening for latent tuberculosis infection ( LTBI ) is a complex consideration that varies by institution. Inconsistent performance of interferon‐gamma release assay IGRA further complicates screening. Data regarding outcomes and test characteristics in large, foreign‐born pre‐transplant population within the United States are limited. Methods In this retrospective study, patients who received Quanti FERON ® ‐ TB Gold QFT prior to liver transplantation...
Whole-genome sequencing (WGS) of Staphylococcus aureus is increasingly used as part infection prevention practices. In this study, we established a long-read technology-based WGS screening program all first-episode methicillin-resistant (MRSA) blood infections at major urban hospital. A survey 132 MRSA genomes assembled from long reads enabled detailed characterization an outbreak lasting several months CC5/ST105/USA100 clone among 18 infants in neonatal intensive care unit (NICU). Available...
Methicillin-resistant Staphylococcus aureus (MRSA) causes life-threatening infections in both community and hospital settings is a leading cause of health care-associated (HAIs). We sought to describe the molecular epidemiological landscape patients with MRSA bloodstream (BSIs) at an urban medical center by evaluating clinical characteristics associated two dominant endemic clones.Comprehensive data from electronic records 227 hospitalized ≥18 years old BSI over 33-month period New York City...
Children's exposure to arsenic and chromium from playground equipment constructed with chromated copper arsenate (CCA)-treated wood is a potential concern because of children's hand-to-mouth activity. However, there exists no direct measure Cr levels on the hands children after playing in such playgrounds. In this study we measured both soluble total 139 playgrounds, eight which were CCA-treated not. age duration play recorded. The each child washed 150 mL deionized water, was collected bag...