A5005813650- Zebrafish Biomedical Research Applications
- Axon Guidance and Neuronal Signaling
- Neuroscience and Neuropharmacology Research
- Congenital heart defects research
- Neurobiology and Insect Physiology Research
- Cardiac Fibrosis and Remodeling
- Retinal Development and Disorders
- RNA Research and Splicing
- Neuroinflammation and Neurodegeneration Mechanisms
- Genomics and Chromatin Dynamics
- Neuroendocrine regulation and behavior
- Neurogenesis and neuroplasticity mechanisms
- Nuclear Structure and Function
- Physiological and biochemical adaptations
- Sodium Intake and Health
- Nuclear Receptors and Signaling
- Cardiovascular Disease and Adiposity
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neural Engineering
- Adipose Tissue and Metabolism
- Congenital Heart Disease Studies
- Bioactive Compounds and Antitumor Agents
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Circular RNAs in diseases
- Photoreceptor and optogenetics research
Max Delbrück Center
2017-2024
Max Planck Institute of Neurobiology
2007-2017
University of California, San Francisco
2012-2013
Telethon Institute Of Genetics And Medicine
2004-2006
Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Recent efforts in protein engineering have significantly increased the performance of GECIs. The state-of-the art single-wavelength GECI, GCaMP3, has been deployed a number model organisms and can reliably detect three or more action potentials short bursts several vivo . Through structure determination, targeted mutagenesis, high-throughput screening, battery vitro assays, we dynamic range GCaMP3 by...
The axons of retinal ganglion cells (RGCs) form topographic connections in the optic tectum, recreating a two-dimensional map visual field midbrain. RGC are also targeted to specific positions along laminar axis tectum. Understanding sensory transformations performed by tectum requires identification rules that control formation synaptic laminae axons. However, there is little information regarding spatial relationships between multiple as they establish and retinotopic arborization fields...
Abstract Genetic access to small, reproducible sets of neurons is key an understanding the functional wiring brain. Here we report generation a new Gal4- and Cre-driver resource for zebrafish neurobiology. Candidate genes, including cell type-specific transcription factors, neurotransmitter-synthesizing enzymes neuropeptides, were selected according their expression patterns in small unique subsets from diverse brain regions. BAC recombineering, followed by Tol2 transgenesis, was used...
Cells migrate via diverse pathways and in different modes to reach their final destinations during development. Tangential migration has been shown contribute significantly the generation of neuronal diversity mammalian telencephalon. GABAergic interneurons are best-characterized neurons that tangentially, from ventral telencephalon, dorsally into cortex. However, molecular mechanisms nature these migratory only just beginning be unravelled. In this study we have first identified a novel...
The transcription factor COUP-TFI (NR2F1), an orphan member of the nuclear receptor superfamily, is important regulator neurogenesis, cellular differentiation and cell migration. In forebrain, controls connectivity between thalamus cortex neuronal tangential migration in basal telencephalon. Here, we show that required for proper axonal growth guidance all major forebrain commissures. Fibres corpus callosum, hippocampal commissure anterior project aberrantly fail to cross midline null...
Animals constantly need to judge the valence of an object in their environment: is it potential food or a threat? The brain makes fundamental decisions on appropriate behavioral strategy by integrating external information from sensory organs and internal signals related physiological needs. For example, hungry animal may take more risks than satiated one when deciding approach avoid object. Using proteomic profiling approach, we identified Calmodulin-interacting peptide Pcp4a as key...
The autonomic nervous system plays a pivotal role in cardiac repair. Here, we describe the mechanistic underpinning of adrenergic signaling fibrotic and regenerative response heart to be dependent on immunomodulation. A pharmacological approach identified receptor alpha-1 as key regulator macrophage phenotypic diversification following myocardial damage zebrafish. Genetic manipulation single-cell transcriptomics showed that signals activation an "extracellular matrix remodeling"...
Nucleoporins build the nuclear pore complex (NPC), which, as sole gate for nuclear-cytoplasmic exchange, is of outmost importance normal cell function. Defects in process nucleocytoplasmic transport or its machinery have been frequently described human diseases, such cancer and neurodegenerative disorders, but only a few cases developmental disorders. Here we report biallelic mutations nucleoporin NUP88 novel cause lethal fetal akinesia deformation sequence (FADS) two families. FADS...
Cells can sacrifice their individuality by fusing, but the prevalence and significance of this process are poorly understood. To approach these questions, here we generate transgenic reporter lines in zebrafish to label specifically ablate fused cells. In addition skeletal muscle cells, reporters cardiomyocytes starting at an early developmental stage. Genetic mosaics generated cell transplantation show expressing both donor- host-derived transgenes, confirming occurrence fusion larval...
Stressful experiences early in life can increase the risk of cardiovascular diseases. However, it remains largely unknown how stress influences susceptibility to disease onset. Here, we show that exposure brain-processed disrupts myocardial growth by reducing cardiomyocyte mitotic activity. Activation glucocorticoid receptor (GR), primary response pathway, reduces numbers, trabecular formation, and leads contractile dysfunction developing myocardium. a physiological level GR signaling is...
Abstract Aims Dysregulated immune response contributes to inefficiency of treatment strategies control hypertension and reduce the risk end-organ damage. Uncovering pathways driving transition from onset hypertensive stimulus manifestation multi-organ dysfunction are much-needed insights for targeted therapy. Methods results To aid visualization cellular events orchestrating pathogenesis, we modelled cardiovascular remodelling in zebrafish. Zebrafish larvae exposed ion-poor environment...
Animals adapt their behaviors to ever-changing needs. Internal states, such as hunger, fear, stress, and arousal are important behavioral modulators controlling the way an organism perceives sensory stimuli reacts them. The translucent zebrafish larva is ideal model for studying neuronal circuits regulating brain owning possibility of easy imaging manipulating activity genetically identified neurons while animal performs stereotyped well-characterized behaviors. main neuromodulatory present...
The larval zebrafish has emerged as a very useful model organism to study the neuronal circuits controlling neuroendocrine and behavioral responses stress. This protocol describes how expose larvae hyperosmotic stress test whether candidate populations of neurons are activated or inhibited by stressor using relatively rapid immunofluorescence staining approach. approach takes advantage phosphorylation extracellular signal-regulated kinase (ERK) upon activation. For complete details on use...
Epicardial adipose tissue (eAT) is a metabolically active fat depot that has been associated with wide array of cardiac homeostatic functions and cardiometabolic diseases. A full understanding its diverse physiological pathological roles hindered by the dearth animal models. Here, we show, in heart an ectothermic teleost, zebrafish, existence localized underneath epicardium, originating from epicardium exhibiting molecular signature beige adipocytes. Moreover, subset adipocytes within this...
Abstract Nucleoporins build the nuclear pore complex (NPC), which, as sole gate for nuclear-cytoplasmic exchange, are of outmost importance normal cell function. Defects in process nucleocytoplasmic transport or its machinery have been frequently described human diseases, such cancer and neurodegenerative disorders, but only a few cases developmental disorders. Here we report biallelic mutations nucleoporin NUP88 novel cause lethal fetal akinesia deformation sequence (FADS) two families....