A5073839152- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- Liquid Crystal Research Advancements
- T-cell and B-cell Immunology
- Transplantation: Methods and Outcomes
- Renal Transplantation Outcomes and Treatments
- CAR-T cell therapy research
- Photoreceptor and optogenetics research
- Acute Lymphoblastic Leukemia research
- Organ Transplantation Techniques and Outcomes
- Cytomegalovirus and herpesvirus research
- Organometallic Complex Synthesis and Catalysis
- Molecular spectroscopy and chirality
- Coordination Chemistry and Organometallics
- Surfactants and Colloidal Systems
- Polyomavirus and related diseases
- Neuroblastoma Research and Treatments
- Acute Myeloid Leukemia Research
- Mesenchymal stem cell research
- Planetary Science and Exploration
- RNA Interference and Gene Delivery
- Synthesis and characterization of novel inorganic/organometallic compounds
- Spacecraft and Cryogenic Technologies
- Electron Spin Resonance Studies
- Organophosphorus compounds synthesis
Robert Bosch (Germany)
2023
University Children's Hospital Tübingen
2007-2020
Klinik Donaustauf
2018
University Hospital Regensburg
2018
Boston Children's Hospital
2007-2015
University of Tübingen
2005-2015
Airbus (Germany)
2015
Goethe University Frankfurt
2012-2014
University Medical Center Hamburg-Eppendorf
2013
Universität Hamburg
2013
Atomic force microscopy and single-molecule spectroscopy were combined to image manipulate purple membrane patches from Halobacterium salinarum. Individual bacteriorhodopsin molecules first localized then extracted the membrane; remaining vacancies imaged again. Anchoring forces between 100 200 piconewtons for different helices found. Upon extraction, found unfold. The spectra revealed individuality of unfolding pathways. Helices G F as well E D always unfolded pairwise, whereas B C...
We report on posttransplant relapsed pediatric patients with B-precursor acute lymphoblastic leukemia no further standard of care therapy who were treated the T-cell engaging CD19/CD3-bispecific single-chain antibody construct blinatumomab a compassionate use basis. Blast load was assessed prior to, during and after cycle using flow cytometry to detect minimal residual disease, quantitative polymerase chain reaction for rearrangements immunoglobulin or receptor genes, bcr/abl mutation...
Summary Neonates show an impaired anti-microbial host defence, but the underlying immune mechanisms are not understood fully. Myeloid-derived suppressor cells (MDSCs) represent innate cell subset characterized by their capacity to suppress T immunity. In this study we demonstrate that a distinct MDSC with neutrophilic/granulocytic phenotype (Gr-MDSCs) is highly increased in cord blood compared peripheral of children and adults. Functionally, isolated Gr-MDSCs suppressed proliferation...
Summary Transplantation of T ‐ and B ‐cell depleted allografts from haploidentical family donors was evaluated within a prospective phase II trial in children with acute lymphoblastic leukaemia, myeloid leukaemia advanced myelodysplastic syndrome ( n = 46). 20 patients had active disease; 19 received second or third stem cell transplantation SCT ). Toxicity‐reduced conditioning regimens consisted fludarabine clofarabine (in disease only), thiotepa, melphalan serotherapy. Graft manipulation...
Herpes simplex virus type 1 (HSV‐1) enters sensory neurons and can remain latent there until reactivation. During latency restricted HSV‐1 gene expression takes place in the form of latency‐associated transcripts (LAT). LAT has been demonstrated to be important not only for but also reactivation, which may cause cranial nerve disorders. Tissue sections trigeminal ganglia (TG), geniculate (GG), vestibular (VG) from seven subjects were examined presence using situ hybridization technique. was...
In the malaria parasite Plasmodium falciparum isoprenoid precursors are synthesised inside a plastid‐like organelle (apicoplast) by mevalonate independent 1‐deoxy‐ d ‐xylulose‐5‐phosphate (DOXP) pathway. The last reaction step of DOXP pathway is catalysed LytB enzyme which contains [4Fe–4S] cluster. this study, P. was shown to be catalytically active in presence an NADPH dependent electron transfer system comprising ferredoxin and ferredoxin‐NADP + reductase. were found form stable protein...
Background Natural killer cells have been demonstrated to exert remarkable graft-versus-leukemia effects after haploidentical transplantation. Acquisition of both, inhibiting and activating, receptors on developing natural is an important step in their functional maturation. Here, we report the reconstitution transplantation T-and B-cell (CD3/CD19) depleted grafts with co-transfusion children its influence cell activity clinical outcome.Design Methods We analyzed patterns at different time...
Transplantation of hematopoietic stem cells from mismatched related donors makes a potential donor available for every child in need cell transplantation. Here, we compare three different graft manipulation methods patients with leukemias and lymphomas: positive selection either CD34 (n = 39) or CD133-coated magnetic microbeads 14) new strategy which depletes T- B-cells through the use CD3- CD19-coated 11). Median purity was comparable after CD34+-selection CD133+-selection, whereas were...
Pediatric patients with relapsed metastatic tumors have a poor prognosis and new treatment strategies are warranted. We present preliminary results of pilot study, evaluating the feasibility toxicity transplantation haploidentical T B cell depleted grafts high numbers NK cells. 6 neuroblastomas (n = 4), rhabdomyosarcoma 1) or Ewing's sarcoma after previous autologous received CD3/CD19 from mismatched family donors median number 16 × 106/kg stem cells, 167 Natural Killer cells only 5.4 104/kg...
In acute myeloid leukemia (AML), the leukemia-initiating cell is found within CD34+/CD38− compartment. Over last years evidence grew that AML initiated and propagated by leukemic stem cells (LSCs). Conceivably, these most immature are more resistant to therapy subsequently initiate relapse. The authors studied 17 patients with childhood treated according AML-BFM 98/04 protocol. At diagnosis, determined characteristic immunophenotype of flow cytometry investigated expression CD34, CD38, CD45...
Zusammenfassung Hintergrund Interstitielle Lungenerkrankungen (ILD) umfassen verschiedenste heterogene, zumeist chronische Erkrankungen des Interstitiums und/oder der Alveolen mit bekannten und unbekannten Ursachen. Die Diagnostik ILD ist sehr komplex sollte interdisziplinär erfolgen. Eine wesentlichen Basisuntersuchungen eine umfangreiche Anamnese. Hierzu kam im deutschsprachigen Raum bisher Frankfurter Bogen von 1985 zur Anwendung, mittlerweile jedoch sprachlich inhaltlich einer...