A5050791399- Cancer-related molecular mechanisms research
- Genomics and Phylogenetic Studies
- Chemical Synthesis and Analysis
- Sphingolipid Metabolism and Signaling
- Tea Polyphenols and Effects
- Inflammasome and immune disorders
- Palliative Care and End-of-Life Issues
- Food Quality and Safety Studies
- Carbohydrate Chemistry and Synthesis
- Molecular Biology Techniques and Applications
- Medical Image Segmentation Techniques
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Genetics, Aging, and Longevity in Model Organisms
- Genetic Mapping and Diversity in Plants and Animals
- Robotics and Sensor-Based Localization
- Bat Biology and Ecology Studies
- Neurological Disease Mechanisms and Treatments
- Synthesis of Organic Compounds
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Image and Object Detection Techniques
- Synthesis and Biological Evaluation
- Emergency and Acute Care Studies
First Affiliated Hospital of Anhui Medical University
2020-2025
Anhui Medical University
2018-2025
Sichuan University
2021-2024
Guangzhou University of Chinese Medicine
2023-2024
Cayman Chemical (United States)
2024
Seattle University
2023
Institute for Systems Biology
2023
Northwestern Polytechnical University
2023
Chinese Academy of Sciences
2014-2022
Kunming Institute of Zoology
2016-2022
Tea is the world's oldest and most popular caffeine-containing beverage with immense economic, medicinal, cultural importance. Here, we present first high-quality nucleotide sequence of repeat-rich (80.9%), 3.02-Gb genome cultivated tea tree Camellia sinensis. We show that an extraordinarily large size resulted from slow, steady, long-term amplification a few LTR retrotransposon families. In addition to recent whole-genome duplication event, lineage-specific expansions genes associated...
To understand the potential genetic basis of highland adaptation fungal pathogenicity, we present here ~116 Mb de novo assembled high-quality genome Ophiocordyceps sinensis endemic to Qinghai-Tibetan Plateau. Compared with other plain-dwelling fungi, find about 3.4-fold inflation O. due a rapid amplification long terminal repeat retrotransposons that occurred ~38 million years ago in concert uplift plateau. We also observe massive removal thousands genes related transport process and energy...
Rapidly driven by the need for developing sustainable sources of nutritionally important fatty acids and rising concerns about environmental impacts after using fossil oil, oil-plants have received increasing awareness nowadays. As an oil-rich plant in China, Camellia oleifera has played a vital role providing nutritional applications, biofuel productions chemical feedstocks. However, lack C. genome sequences little genetic information largely hampered urgent needs efficient utilization...
Abstract Prokaryotes possess a simple genome transcription system that is different from of eukaryotes. In chloroplasts (plastids), it believed the prokaryotic gene features govern transcription. However, polycistronic operon model cannot account for all chloroplast (plastome) products at whole-genome level, especially regarding various RNA isoforms. By systematically analyzing transcriptomes plastids algae and higher plants cyanobacteria, we find entire plastome transcribed in...
Simple Sequence Repeats (SSRs), also known as microsatellites, are ubiquitous short tandem duplications commonly found in genomes and/or transcriptomes of diverse organisms. They represent one the most powerful molecular markers for genetic analysis and breeding programs because their high mutation rate neutral evolution. However, traditionally experimental screening SSR polymorphic status subsequent applicability to studies extremely labor-intensive time-consuming. Thankfully, recently...
Camellia taliensis is one of the most important wild relatives cultivated tea tree, C. sinensis. The species extensively occupies mountainous habitats representing a wide-range abiotic tolerance and biotic resistance thus harbors valuable gene resources that may greatly benefit genetic improvement tree. However, owning to large genome size ~3 Gb structurally complex genome, there are fairly limited information particularly few genomic publicly available for this species. To better understand...
// Shao-Yan Pu 1, 2, * , Qin Yu 3, Huan Wu 3 Jian-Jun Jiang Xiao-Qiong Chen 2 Yong-Han He and Qing-Peng Kong 1 State Key Laboratory of Genetic Resources Evolution, Kunming Institute Zoology, The Chinese Academy Sciences, 650223, China KIZ/CUHK Joint Bioresources Molecular Research in Common Diseases, College Life Science, University Beijing 100049, These authors have contributed equally to this work Correspondence to: Kong, email: kongqp@mail.kiz.ac.cn He, heyonghan@mail.kiz.ac.cn Keywords:...
Growth differentiation factor (GDF) 11 levels decline with aging.The age-related loss of GDF has been implicated in the pathogenesis a variety diseases.GDF11 supplementation reversed cardiac hypertrophy, bone loss, and pulmonary dysfunction old mice, suggesting that GDF11 rejuvenating effect.Less is known about potential to improve recovery after an acute injury, such as stroke, aged mice.GDF11/8 were assessed young male mice postmortem human brain samples.Aged subjected transient middle...
This study aimed to explore the effects of ceramide (Cer) on NLRP3 inflammasome activation and their underlying mechanisms.Lipopolysaccharide (LPS)/adenosine triphosphate (ATP)-induced in J774A.1 cells THP-1 macrophages was used as an vitro model inflammation. Western blotting real-time PCR (RT-PCR) were detect protein mRNA levels, respectively. IL-1β IL-18 levels measured by ELISA. ASM assay kit immunofluorescence activity Cer content.Imipramine, a well-known inhibitor ASM, significantly...
Introduction Triptolide (TPL) is a promising plant-derived compound for clinical therapy of multiple human diseases; however, its application was limited considering toxicity. Methods To explore the underlying molecular mechanism TPL nephrotoxicity, network pharmacology based approach utilized to predict candidate targets related with toxicity, followed by deep RNA-seq analysis characterize features three transcriptional elements include protein coding genes (PCGs), long noncoding RNAs...
Abstract A comparative study of the effectiveness selected coupling reagents in dipeptide synthesis was conducted. Included were a new reagent, pentafluorophenyl diphenylphosphinate (FDPP, 1), benzotriazol-1-yl-oxytris(dimethylamino) phosphonium hexafluorophosphate (BOP, 2), 2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium (HBTU, 3), and isobutyl chloroformate (IBCF, 4). We found that BOP (2) afforded highest yields lowest limits racemization.
Jie Jiangab, Yong Zhaa*, Jay Gaoc & Jianjun Jianga a Key Laboratory of Virtual Geographic Environment Ministry Education, College Science, Nanjing Normal University , Nanjing, 210097, PR China b and Biological Information, Posts Telecommunications 210003, c School Geography, Geology Environmental Auckland Auckland, 1008, New Zealand
The CRISPR/Cas9 system has been successfully applied in dozens of diverse species; although the screening successful editing events remains particularly laborious, especially for those that occur at relatively low frequency. Recently, a co-CRISPR strategy was proved to enrich desired CRISPR events. Here, developed Fall armyworm, Spodoptera frugiperda, with kynurenine 3-monooxygenase gene (kmo) as marker. kmo mosaics induced by single-guide RNAs (sgRNAs)/Cas9 displayed darker green color...
Abstract African wild rice Oryza longistaminata , one of the eight AA‐ genome species in genus possesses highly valued traits, such as rhizomatousness for perennial breeding, strong tolerance to biotic and abiotic stresses, high biomass production on poor soils. To obtain high‐quality reference O. we employed a hybrid assembly approach through incorporating Illumina PacBio sequencing datasets. The final comprised only 107 scaffolds was approximately ∼363.5 Mb, representing ∼92.7% estimated...
Long noncoding RNAs (lncRNAs) are a class of ubiquitous and have been found to act as tumor suppressors or oncogenes, which dramatically altered our understanding cancer. Naked mole rat (NMR, Heterocephalus glaber) is an exceptionally long-lived cancer-resistant rodent; however, whether lncRNAs play roles in cancer resistance this seductive species remains unknown. In study, we developed pipeline identified total 4422 across the NMR genome based on 12 published transcriptomes. Systematic...
DNA replication is precisely regulated in cells and its dysregulation can trigger tumorigenesis. Here we identified that the TOPBP1 interacting checkpoint regulator (TICRR) mRNA level was universally highly expressed 15 solid cancer types. Depletion of TICRR significantly inhibited tumor cell growth, colony formation migration vitro, strikingly growth xenograft model. We reveal knockdown not only initiation but also fork progression replication. Suppression synthesis by silencing caused...
Aging is the largest risk factor of major human diseases. Long noncoding RNAs (lncRNAs) as key regulatory elements have shown a strong impact on multiple biological processes well disease mechanisms. However, roles lncRNAs in aging/healthy aging remain largely unknown. Centenarians are good models for healthy studies due to avoiding chronic diseases and disabilities. To illustrate their ubiquitous nature genome 'secrets' regulation from perspective lncRNAs, peripheral blood samples two...