A5048684984- Glycosylation and Glycoproteins Research
- Wound Healing and Treatments
- Mycotoxins in Agriculture and Food
- Cytomegalovirus and herpesvirus research
- Ubiquitin and proteasome pathways
- Parasitic Diseases Research and Treatment
- Invertebrate Immune Response Mechanisms
- Neurobiology and Insect Physiology Research
- Health and Medical Studies
- Toxin Mechanisms and Immunotoxins
- Herpesvirus Infections and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- T-cell and Retrovirus Studies
- Muscle Physiology and Disorders
- Dermatological diseases and infestations
- Aquaculture disease management and microbiota
- Toxoplasma gondii Research Studies
- Livestock and Poultry Management
- Animal Disease Management and Epidemiology
- Electrospun Nanofibers in Biomedical Applications
- Hydrogels: synthesis, properties, applications
- Pickering emulsions and particle stabilization
- Algal biology and biofuel production
- Immune Cell Function and Interaction
- Viral gastroenteritis research and epidemiology
Bulgarian Academy of Sciences
2015-2025
Morpho (United States)
2016-2024
Institute of Molecular Biology
1990-2022
Institute of Organic Chemistry with Centre of Phytochemistry
2021-2022
Institute of Physical Chemistry
2021-2022
University of Architecture, Civil Engineering and Geodesy
2021-2022
University of Forestry
2021-2022
MSH Medical School Hamburg – University of Applied Sciences and Medical University
2020
Catheter-associated urinary tract infections (CAUTIs), caused by biofilms, are the most frequent health-care associated infections. Novel antibiofilm coatings needed to increase catheters' life-span, decrease prevalence of CAUTIs and reduce development antimicrobial resistance. Herein, antibacterial zinc oxide nanoparticles (ZnO NPs) were decorated with a biofilm matrix-degrading enzyme amylase (AM) simultaneously deposited onto silicone catheters in one-step sonochemical process. The...
Chronic wounds are persistent non-healing lesions whose complex management is due to the interplay of multiple factors promoting chronicity, such as oxidative stress, overexpressed enzyme activities impeding tissue repair and bacterial contamination. Currently marketed chronic wound dressings designed mainly absorb exudate, at most release antimicrobial agents, usually ionic silver. However, an effective requires addressing multifactorial nature in a holistic approach. This study explores...
Catheter-associated urinary tract infections are the most common hospital-acquired and cause patient discomfort, increased morbidity, prolonged stays, altogether posing a huge burden on healthcare services. Colonization occurs upon insertion, or later by ascending microbes from rich periurethral flora, is therefore virtually unavoidable medical procedures. Importantly, dwell time significant risk factor for bacteriuria because it gives biofilms to develop mature. This why we engineer...
Hemocyanins are oxygen-transporting glycoproteins in the hemolymph of arthropods and mollusks that attract scientific interest with their diverse biological activities potential applications pharmacy medicine. The aim present study was to assess vitro antitumor activity hemocyanins isolated from marine snail Rapana venosa (RvH) garden snails Helix lucorum (HlH) aspersa (HaH), as well mucus H. snails, HT-29 human colorectal carcinoma cell line. effects on viability proliferation were analyzed...
The free radical nitric oxide (NO) and Ca
Catheter-associated urinary tract infections represent a major share of nosocomial infections, and are associated with longer periods hospitalization huge financial burden. Currently, there only handful commercial materials that reduce biofilm formation on catheters, mostly relying silver alloys. Therefore, we combined silver-phenolated lignin nanoparticles poly(carboxybetaine) zwitterions to build composite antibiotic-free coating bactericidal antifouling properties. Importantly, the...
The aim of this study was to investigate the role human herpesvirus-6 (HHV-6) in autoimmune thyroiditis (AIT) development. We examined possible involvement HHV-6 gene expression encoding immunomodulating proteins U12 and U51 AIT development their modulation chemokine signaling. One hundred patients with following thyroidectomy were enrolled study. Nested polymerase chain reaction (nPCR) used detect sequence DNA samples. Reverse transcription PCR (RT-PCR) three different targets (U79/80, U12)...
The study reports the synthesis and characterization of novel triple stimuli responsive interpenetrating polymer network (IPN) based on two polyzwitterionic networks, namely poly(carboxybetaine methacrylate) poly(sulfobetaine methacrylate). zwitterionic IPN hydrogel demonstrates ability to expand or shrink in response changes three "biological" external such as temperature, pH, salt concentration. shows good mechanical stability. In addition, other important features non-cytotoxicity...
Hemocyanins are oxygen-transporting glycoproteins in the hemolymph of some invertebrate species that attracted scientific interest as potential anticancer agents. The present study aims to assess vitro and vivo activity hemocyanins isolated from Helix aspersa, lucorum, Rapana venosa Graffi myeloid tumor model. antitumor was determined by a MTT test cytomorphological analysis fluorescent transmission electron microscopy. effects were examined hamsters transplanted with tumor. serum antibody...
Abstract Rabbits and rats were inoculated with material derived from FLK cells producing permanently bovine leukaemia virus (BLV). The viral presence in the inoculum was proved by transmission electron microscopy, immunofluorescence, immunogold labelling demonstrating Tax protein, PCR analysis. About 30 % of infected animals sustained BLV seropositivity during experiment, demonstrated symptoms lympholeukaemia - clinical manifestation an immunosuppressive condition, increased number...
Pathomorphological changes in the lungs, stomach, and small intestines of wild boars infected with Metastrongylus spp., Ascarops strongylina, Macracanthorhynchus hirudinaceus were investigated.Dissection 11 was performed, parasitised organs histologically investigated by common techniques.Macroscopic lesions lungs Metastrongyus spp. seen within apical parts large lobes, irregular form, pale greyish colour, compact consistency. The main pathohistological findings were: presence parasite...
The sialylation of the glycoproteins in skeletal muscle tissue is not well investigated, even though essential role sialic acids for proper muscular function has been proven by many researchers. invasion parasitic nematode Trichinella spiralis muscles with subsequent formation Nurse cell-parasite complex initiates increased accumulation sialylated within affected area fiber. aim this study to describe some details α-2,6-sialylation invaded cells. Asynchronous infectious T. larvae was...
The Nurse cell of the parasitic nematode Trichinella spiralis is a unique structure established after genetic, morphological and functional modification small portion invaded skeletal muscle fiber. Even if newly developed cytoplasm no longer contractile, this remains well integrated within surrounding healthy tissue. Our previous reports suggested that process accompanied by an increased local biosynthesis sialylated glycoproteins. In work we examined expressions three proteins, functionally...
Microalgae are of great importance for the production original natural substances interest food, health or biotechnological applications. Mass cultures green microalgae used carotenoids such β-carotene, astaxanthin, canthaxanthin, lutein etc. The aim this study was to obtain an oil extract from a Bulgarian microalga strain Coelastrella sp. BGV and assess its anticancer apoptogenic activity in vitro against human tumor cells HeLa by means MTT fluorescence microscopy analyses. results showed...
A case of trichuriasis in three zoo-keeping baboons is described.One the animals had a high degree infection and marked clinical symptoms, other two -low good general condition.The were treated according to regimens: 1. Albendazole (7.5 mg/kg) orally for days; 2. Combination albendazole ivermectin (0.4 mg/kg), both drugs days.Despite treatment, condition first baboon deteriorated he was euthanized.The postmortem examination revealed pathomorphological changes intestines, liver, kidneys...
Abstract The self‐organization of microbes into biofilms provides multiple benefits including tolerance to mechanical stress and resistance immune defences antibiotics. Coupled a compromised mucociliary function, these traits have dire consequences in cystic fibrosis patients – persistent infections are the main reason for morbidity mortality. Thereby, disease progression is associated with universal colonization by Pseudomonas aeruginosa , which selects slimy phenotype adapt lung...
Escherichia coli 0164 is the most prevalent serotype among enteroinvasive E. in Bulgaria and annually isolated sporadic, as well epidemic cases. We studied 243 strains of this serotype, different regions country over period 1954-1988. The plasmid analysis performed revealed that presence large DNA with molecular size 140 MDa was related to virulence strains, assessed by test SERENY. absence or its deletion led loss virulence. In 92% two small plasmids were demonstrated 6.0 4.8 MDa, which...