A5084695843- RNA modifications and cancer
- RNA Research and Splicing
- SARS-CoV-2 and COVID-19 Research
- Natural product bioactivities and synthesis
- RNA and protein synthesis mechanisms
- Phytochemicals and Antioxidant Activities
- Enzyme Production and Characterization
- Protein Hydrolysis and Bioactive Peptides
- Plant Gene Expression Analysis
- Viral gastroenteritis research and epidemiology
- CRISPR and Genetic Engineering
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Epigenetics and DNA Methylation
- Bacteriophages and microbial interactions
- Cancer-related molecular mechanisms research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Animal Disease Management and Epidemiology
- Genomics and Chromatin Dynamics
- Virus-based gene therapy research
- Phytase and its Applications
- Infection Control and Ventilation
- Chromatography in Natural Products
- Genomics and Phylogenetic Studies
- Phytochemical Studies and Bioactivities
Nanjing Drum Tower Hospital
2011-2025
Chinese Academy of Sciences
2012-2024
Hefei National Center for Physical Sciences at Nanoscale
2024
University of Science and Technology of China
2024
Jiujiang University
2024
Shenzhen Institutes of Advanced Technology
2021-2023
Jiangsu University
2023
State Key Laboratory of Synthetic Chemistry
2023
Guangdong Provincial Center for Disease Control and Prevention
2009-2022
Zunyi Medical University
2020-2022
The reference human genome sequence set the stage for studies of genetic variation and its association with disease, but epigenomic lack a similar reference. To address this need, NIH Roadmap Epigenomics Consortium generated largest collection so far epigenomes primary cells tissues. Here we describe integrative analysis 111 as part programme, profiled histone modification patterns, DNA accessibility, methylation RNA expression. We establish global maps regulatory elements, define modules...
Genome-wide studies have identified thousands of long noncoding RNAs (lncRNAs) lacking protein-coding capacity. However, most lncRNAs are expressed at a very low level, and in cases there is no genetic evidence to support their vivo function. Malat1 (metastasis associated lung adenocarcinoma transcript 1) among the abundant highly conserved lncRNAs, it exhibits an uncommon 3'-end processing mechanism. In addition, its specific nuclear localization, developmental regulation, dysregulation...
The role of fecal aerosols in the transmission severe acute respiratory syndrome coronavirus 2 has been suspected.
Significance Alternative splicing (AS) plays an important role in the mammalian brain, but our atlas of AS events is incomplete. Here, we conducted comprehensive analysis deep RNA-Seq data mouse cortex to identify new and evaluate their functionality. We expanded number annotated more than 10-fold demonstrated that, like many known events, thousands newly discovered are regulated, conserved, likely functional. In particular, some can regulate gene expression levels through nonsense-mediated...
A crucial step in analyzing mRNA-Seq data is to accurately and efficiently map hundreds of millions reads the reference genome exon junctions. Here we present OLego, an algorithm specifically designed for de novo mapping spliced reads. OLego adopts a multiple-seed-and-extend scheme, does not rely on separate external aligner. It achieves high sensitivity junction detection by strategic searches with small seeds (∼14 nt mammalian genomes). To improve accuracy resolve ambiguous at junctions,...
Tree peony (Paeonia section Moutan DC.) is known for its excellent ornamental and medicinal values. In 2011, seeds from P. ostii have been identified as novel resource of α-linolenic acid (ALA) seed oil production development in China. However, the molecular mechanism on biosynthesis unsaturated fatty acids tree remains unknown. Therefore, transcriptome data needed to better understand underlying mechanisms.
Abstract Motivation: Alternative splicing (AS) is a pre-mRNA maturation process leading to the expression of multiple mRNA variants from same primary transcript. More than 90% human genes are expressed via AS. Therefore, quantifying inclusion level every exon crucial for generating accurate transcriptomic maps and studying regulation Results: Here we introduce SpliceTrap, method quantify levels using paired-end RNA-seq data. Unlike other tools, which focus on full-length transcript isoforms,...
Anthocyanins are major pigments in plants. Methylation plays a role the diversity and stability of anthocyanins. However, contribution anthocyanin methylation to flower coloration is still unclear. We identified two homologous O-methyltransferase (AOMT) genes from purple-flowered (PsAOMT) red-flowered (PtAOMT) Paeonia plants, we performed functional analyses vitro vivo. The critical amino acids for AOMT catalytic activity were studied by site-directed mutagenesis. showed that recombinant...
The scarcity of primordial germ cells (PGCs) in the developing mammalian embryo hampers robust biochemical analysis processes that underlie early cell formation. Here, we demonstrate DAZL, a cell-specific RNA binding protein, is PGC marker during vitro development. Using Dazl-GFP reporter ESCs, DAZL plays central role large mRNA/protein interactive network blocks translation core pluripotency factors, including Sox2 and Sall4, as well Suz12, polycomb family member required for...
Flavonoids in nine tissues of Nelumbo nucifera Gaertner were identified and quantified by high-performance liquid chromatography with diode array detector (HPLC-DAD) HPLC-electrospray ionization-mass spectrometry (HPLC-ESI-MSn). Thirty-eight flavonoids identified; eleven C-glycosides five O-glycosides discovered for the first time N. nucifera. Most importantly, C-glycosyl apigenin or luteolin detected lotus plumules proved valuable deep elucidation flavonoid composition further utilization...
To explore the composition of ocular microbiome in normal subjects and patients with Meibomian gland dysfunction (MGD).Seventy (140 eyes) were enrolled our study. Signs dry eye evaluated bacterial species conjunctival sac (CS) (MG) secretions then identified by 16S rRNA gene sequencing. Additionally, 17 (34 further to determine differences microbiomes surface deep layers MG using a segmental secretion analysis.The positive isolation rate was markedly higher than CS. The control mild group...
Glycosylation by uridine diphosphate-dependent glycosyltransferases (UGTs) in plants contributes to the complexity and diversity of secondary metabolites. UGTs are generally promiscuous their use acceptors, making it challenging reveal function vivo. Here, we described an approach that combined glycoside-specific metabolomics precursor isotopic labeling analysis characterize Arabidopsis. We revisited UGT72E cluster, which has been reported catalyze glycosylation monolignols....
SARS-CoV 3a is a structural protein, mainly localizing to Golgi apparatus and co-localizing with M in co-transfected cells. Here we observed that transient expression of inhibited cell growth prevented 5-bromodeoxyuridine incorporation, suggesting deregulated cycle progression. Cell analysis demonstrated was associated blockage progression at G1 phase HEK 293, COS-7, Vero cells 24–60 h after transfection. Mutation revealed C-terminal region (176 aa ∼ 274 aa), including potential calcium...
A disease outbreak with dengue-like symptoms was reported in Guangdong Province, China, October 2010. Testing results confirmed that the pathogen causing chikungunya virus. Phylogenic analysis indicated this virus a member of Indian Ocean clade East/Center/South African subgroup
Waterlily (Nymphaea spp.), a perennial herbaceous aquatic plant, is divided into two ecological groups: hardy waterlily and tropical waterlily. Although the has no attractive blue flower cultivar, its adaptability stronger than because it can survive cold winter. Thus, breeding with real flowers become an important target for breeders. Molecular may be useful way. However, molecular studies on are limited due to lack of sequence data. In this study, six cDNA libraries generated from petals...
Hepatic sarcomatoid carcinoma (HSC) and hepatic sarcoma (HS) are rare malignancies. Without pathology, the differential diagnosis between these two tumors is difficult due to their frequent overlaps in clinical presentations imaging features. Currently, there limited analyses about ultrasound (US), contrast-enhanced (CEUS) computer tomography (CECT) characteristics of HSC HS. Therefore, purpose our study evaluate value US, CEUS CECT on From 2015 2022, a total 23 patients with (n = 11) HS 12)...