A5100730594- Advanced Chemical Sensor Technologies
- Analytical Chemistry and Chromatography
- Pesticide Residue Analysis and Safety
- Smoking Behavior and Cessation
- Analytical chemistry methods development
- Toxic Organic Pollutants Impact
- Effects and risks of endocrine disrupting chemicals
- Water Treatment and Disinfection
- DNA and Nucleic Acid Chemistry
- Electrochemical Analysis and Applications
- Listeria monocytogenes in Food Safety
- Synthesis and Biological Evaluation
- Radiation Effects and Dosimetry
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cell Image Analysis Techniques
- RNA modifications and cancer
- Phytochemicals and Antioxidant Activities
- Sulfur Compounds in Biology
- Cancer Cells and Metastasis
- Microbial Inactivation Methods
- Cancer, Hypoxia, and Metabolism
- Analytical Chemistry and Sensors
- Advanced biosensing and bioanalysis techniques
- Cancer-related molecular mechanisms research
- Chromatography in Natural Products
Zhujiang Hospital
2025
Southern Medical University
2025
Huashan Hospital
2024
Fudan University
2024
Shanghai Center for Brain Science and Brain-Inspired Technology
2024
Shanghai Clinical Research Center
2024
China Tobacco
2012-2024
National University of Singapore
2019-2022
Xidian University
2021
Nanchang University
2017-2021
Tobacco Heating System 2.2 (THS 2.2, marketed as iQOS) is a heat-not-burn (HNB) tobacco product that has been successfully introduced to global markets. Despite its expanding market, few independent and systematic researches into THS have carried out date. We tested comprehensive list of total particulate matter (TPM), water, tar, nicotine, propylene glycol, glycerin, carbon monoxide, volatile organic compounds, aromatic amines, hydrogen cyanide, ammonia, N-nitrosamines, phenol, polycyclic...
Although cigar tobacco leaves (CTLs) have a high economic value, research regarding the flavor characteristics of CTLs is currently limited. A comprehensive study from different regions China was conducted by identifying their volatile-flavor-containing compounds (VFCs) and flavors. The samples were analyzed via gas chromatography–ion mobility spectrometry (GC–IMS) sensory evaluation. Results revealed considerable differences in VFC contents China, suggesting that VFLs could be influenced...
Abstract Evaluating the source of nicotine in e‐liquid is a problem. Tobacco‐derived contains predominantly (S)‐(−)‐nicotine, whereas tobacco‐free products may not. Thus, we developed new normal phase high‐performance liquid chromatography method to determinate enantiomeric composition 10 kinds flue‐cured tobacco, 3 burley, 1 kind cigar 2 oriental 5 Virginia cigarette, blend e‐liquid, and 4 smokeless tobacco. The amount (R)‐(+)‐nicotine ranged from ~0.02% ~0.76% total nicotine. An sample had...
While previous single-cell RNA sequencing (scRNA-seq) studies have attempted to dissect intracranial aneurysm (IA), the primary molecular mechanism for IA pathogenesis remains unknown. Here, we uncovered alterations of cellular compositions, especially transcriptome changes vascular endothelial cells (ECs), in human IA.
Our preliminary RNA-Seq data revealed altered expression of small nucleolar RNA host gene 9 (SNHG9) in osteoarthritis (OA) and its reverse correlation with miR-34a, which can regulate chondrocyte apoptosis rat OA model. This study was therefore carried out to investigate the potential interaction between SNHG9 miR-34a OA.A total 60 healthy volunteers (Control group) as well patients (OA were enrolled this study. Transfections, RT-qPCR, methylation-specific PCR (MSP) cell assay performed.We...
Acidic compounds constitute a group of chemicals present in mainstream cigarette smoke, among which organic acids contribute to flavoring. In order obtain comprehensive understanding the constituents acidic both particulate and gaseous phases smoke commercial cigarettes, delineate difference between two types yields from nine cigarettes brands (L- M-types) were collected analyzed detail by gas chromatography–mass spectrometry (GC-MS). The results identified quantitatively 46 compounds,...
OTU domain-containing protein 3 (OTUD3), a deubiquitinating enzyme, has been shown to participate in progression of multiple malignancies. The accurate function OTUD3 hepatocellular carcinoma (HCC) remains elusive. We found that was significantly overexpressed HCC, and higher expression correlated with larger tumor size, more distant metastasis, worse TNM stage. A series gain- loss-of-function assays were also performed examine the oncogenic promoting HCC cell growth metastasis vitro. Using...
The accurate determination of the free nicotine content in tobacco is great importance for quality assessment leaves and cigarettes, as well study tar reduction cigarette formulation. A method based on solvent extraction gas chromatography-mass spectrometry (GC-MS) was established direct tobacco. Free from optimized, using different solvents physical techniques (shaking, ultrasonic oscillation, static extraction). Ultimately, a 24 h with cyclohexane, followed by GC-MS analysis, gave superior...
Osteosarcoma is the most common type of malignant tumor arising from bone in children and adolescents. Accumulating evidences have shown aberrant expression numerous miRNAs associated with development metastasis osteosarcoma. The present study was conducted to investigate miR-27a osteosarcoma tissues cells. In study, quantitative RT-qPCR used measure levels miRNA mRNA Transwell assays were detect effects on invasive migratory potential Luciferase reporter western blot analysis confirm cyclin...
Base-T-specific recognition element: A chiral aliphatic amino acid, (S)-β-hydroxyl-γ-aminobutyric when incorporated into polyamides, can act as a novel T-specific element in DNA minor groove sequence-specific event. Good flexibility of the T-reader implies that it could be longer polyamides to regulate their curvature and thus allow discrimination sequences. Detailed facts importance specialist readers are published ”Supporting Information”. Such documents peer-reviewed, but not copy-edited...
Understanding the mechanisms underlying diversity of tumor invasion dynamics, including single-cell migration, multicellular streaming, and emergence various collective migration patterns, is a long-standing problem in cancer research. Here we have designed fabricated series microchips containing high-throughput microscale tracks using protein repelling coating technology, which were then covered with thin Matrigel layer. By varying geometrical confinement (track width) microenvironment...
Tobacco mainstream smoke (MSS) and sidestream (SSS), butts, ashes from commercial cigarettes maleic hydrazide (MH) spiked were analyzed for their MH contents. The transfer rates obtained MSS ranged 1.4% to 3.7%, SSS 0.2% 0.9%, butts 1.1% 1.9%. And as expected, is absent in ashes. rate of into the top one during main-stream, side-stream, ashes, higher levels lead more smoke. Further, analysis total along with that indicates much destructed smoking process.
Abstract Tobacco‐specific N ‐nitrosamines are carcinogenic components in mainstream cigarette smoke. To explore tobacco‐specific ‐nitrosamine release levels cigarettes, a magnetic solid‐phase extraction procedure using graphene composite as sorbent for fast enrichment of was developed. Under optimal conditions, determination method successfully proposed by combining and high‐performance liquid chromatography coupled with tandem mass spectrometry. The method's limit detection smoke ranged...
Abstract A mixed‐mode hydrophobic/cation‐exchange sorbent was used for the solid‐phase extraction of tobacco‐specific N‐nitrosamines. Several parameters including sample solution pH, washing solution, type and volume desorption amount were investigated. By combining high‐performance liquid chromatography tandem mass spectrometry, we successfully developed a method N‐nitrosamines determination. The limit detection target analytes in our ranged from 0.012 to 0.037 ng/mL, correlation...
A simple, rapid, and environmentally friendly method was developed for the determination of acrylamide trimethylolpropane in paper packaging materials. No organic solvent used matrix effect investigated. The extract directly analyzed by liquid chromatography with tandem mass quantification confirmation. chromatographic separations were performed on a ZORBAX HILIC Plus (2.1 mm × 150 mm, 3μm; Agilent, USA) column only one mobile phase (100% water). Calibration curves trimethylopropane achieved...
Abstract A method to determine residues of the three acid herbicides, 2,4,5-trichlorophenoxyacetic acid, 2,4-dichlorophenoxyacetic and 3,6-dichloro-2-methoxybenzoic (dicamba), in tobacco using LC/MS/MS is presented. Because these herbicide might exist different forms (free salt, ester), samples were first pretreated by alkaline hydrolysis then pH was adjusted order convert completely their free forms. Dichloromethane extraction dispersive SPE C18 sorbent carried out before analysis,...