A5070808932- Advanced Glycation End Products research
- Telomeres, Telomerase, and Senescence
- DNA Repair Mechanisms
- Diet and metabolism studies
- Diet, Metabolism, and Disease
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Epigenetics and DNA Methylation
- Diabetes and associated disorders
- BRCA gene mutations in cancer
- Folate and B Vitamins Research
- Dietary Effects on Health
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Occupational and environmental lung diseases
- Genetic factors in colorectal cancer
- Thyroid Cancer Diagnosis and Treatment
- Sinusitis and nasal conditions
- Neonatal Respiratory Health Research
- Autophagy in Disease and Therapy
- Infectious Diseases and Mycology
- Microtubule and mitosis dynamics
- Alcohol Consumption and Health Effects
- DNA and Biological Computing
- Bone Tumor Diagnosis and Treatments
- Radiation Therapy and Dosimetry
- Allergic Rhinitis and Sensitization
Heinrich Heine University Düsseldorf
2017-2024
European Molecular Biology Laboratory
2020-2024
Deutsches Diabetes-Zentrum e.V.
2017-2024
German Center for Lung Research
2024
German Center for Diabetes Research
2017-2024
Ludwig-Maximilians-Universität München
2024
Helmholtz Zentrum München
2017-2024
Heidelberg University
2011-2023
University Hospital Heidelberg
2013-2023
Diabetes Australia
2021
The receptor for advanced glycation endproducts (RAGE) is a multiligand and member of the immunoglobulin superfamily. RAGE mainly involved in tissue damage chronic inflammatory disorders, sustaining response upon engagement with damage-associated molecular pattern molecules (DAMPs) such as S100 proteins high-mobility group box 1 (HMGB1). Enhanced expression its ligands has been demonstrated distinct tumors several studies support crucial role tumor progression metastasis by still unknown...
The integrity of genome is a prerequisite for healthy life. Indeed, defects in DNA repair have been associated with several human diseases, including tissue-fibrosis, neurodegeneration and cancer. Despite decades extensive research, the spatio-mechanical processes double-strand break (DSB)-repair, especially auxiliary factor(s) that can stimulate accurate timely repair, remained elusive. Here, we report an ATM-kinase dependent, unforeseen function nuclear isoform Receptor Advanced Glycation...
Article27 April 2020Open Access Source DataTransparent process Compromised DNA repair is responsible for diabetes-associated fibrosis Varun Kumar Corresponding Author [email protected] orcid.org/0000-0003-2827-1024 Department of Medicine I and Clinical Chemistry, University Hospital Heidelberg, Germany European Molecular Biology Laboratory, Advanced Light Microscopy Facility, German Center Diabetes Research (DZD), Search more papers by this author Raman Agrawal Translational Pulmonology,...
Abstract Context Novel fasting interventions have gained scientific and public attention. Periodic has emerged as a dietary modification promoting beneficial effects on metabolic syndrome. Objective Assess whether periodic reduces albuminuria activates nephropathy-driven pathways. Design/Participants Proof-of-concept study where individuals with type 2 diabetes (n = 40) increased albumin-to-creatinine ratio (ACR) were randomly assigned to receive monthly fasting-mimicking diet (FMD) or...
The adaptive immune system protects organisms from harmful environmental insults. In parallel, regulatory mechanisms control responses in order to assure preservation of organ integrity. Yet, molecules involved the T-cell peripheral tissues are poorly characterized. Here, we investigated function Dickkopf-3 modulation local reactivity. Dkk3 is a secreted, mainly tissue-derived protein with highest expression organs considered as immune-privileged such eye, embryo, placenta, and brain. While...
Non-medullary thyroid cancer (NMTC) is a common endocrine malignancy with genetic basis that has yet to be unequivocally established. In recent whole-genome sequencing study of five families occurrence NMTCs, we shortlisted promising variants the help bioinformatics tools. Here, report in silico analyses and vitro experiments on novel germline variant (p.V29L) highly conserved oligonucleotide/oligosaccharide binding domain Protection Telomeres 1 (POT1) gene one families. The results showed...
Background: Chronic rhinosinusitis (CRS) involves persistent inflammation of the nasal passages and sinuses, often attributed to Aspergillus species as a primary etiological agent. Effective treatment complete eradication fungal pathogens restoration sinus function, commonly achieved through functional endoscopic surgery (FESS). This study aimed evaluate clinical radiological features, assess prevalence identify mycological profile rhinosinusitis. Methods: was prospective observational...
We would like to thank our colleagues Laxy and Emmert-Fees for their balanced careful presentation of a contrary view ("Evidence on the health effect sugar-sweetened beverages taxation: Why it is worth taking really close look!" (MVF 1/2025). If we were discuss instead writing, reader see that there agreement many points with sentences such as "It could be this, but also different".
This study was conducted to investigate the cascade involving DNA damage, senescence, and senescence-associated secretory phenotype (SASP) in experimental diabetes a four-year follow-up patients with pre-diabetes type 2 diabetes.Kidney, lung, liver were studied 4 months diabetic db/db mice age-matched controls for presence of damage fibrosis. (comet-tail-length ɤH2Ax-positivity white blood cells), urinary p21-excretion, plasma IL-6 TGF-β1 determined from 115 healthy participants, 34 221...
We recently demonstrated that the bZip transcription factor nuclear erythroid-derived 2 (Nfe2) represses protein acetylation and expression of glial cell missing 1 (Gcm1) in trophoblast cells, preventing excess syncytiotrophoblast formation permitting normal placental vascularization embryonic growth. However, Gcm1 promoter lacks a Nfe2-binding site hence mechanisms linking Nfe2 remained unknown. Here we show JunD DNA-binding activity to during differentiation. Interventional studies using...
Abstract An elevated frequency of DNA replication defects is associated with diabetes and cancer. However, data linking these nuclear perturbations to the onset or progression organ complications remained unexplored. Here, we report that RAGE (Receptor for Advanced Glycated Endproducts), previously believed be an extracellular receptor, upon metabolic stress localizes damaged forks. There it interacts stabilizes minichromosome-maintenance (Mcm2–7) complex. Accordingly, deficiency leads...
Abstract Non-medullary thyroid cancer (NMTC) is a common endocrine malignancy with genetic basis that has yet to be unequivocally established. In recent whole genome sequencing study of five families recurrence NMTCs, we shortlisted promising variants the help bioinformatics tools. Here, report in silico analyses and vitro experiments on novel germline variant (p.V29L) highly conserved oligonucleotide/oligosaccharide binding domain Protection Telomeres 1 (POT1) gene one families. The results...
The receptor for advanced glycation end-products (RAGE) is a multi-ligand which belongs to the pattern recognition family and can bind various ligands such as (AGEs), members of S100 protein family, glycosaminoglycans, amyloid β peptides, high-mobility group box-1 (HMGB1) nucleic acids through its extracellular domain. RAGE-ligand interaction leads activation MAP kinase NF-kB signaling pathways. Further ligand-induced up-regulation RAGE involved in patho-physiological situations including...
Small cell lung carcinoma (SCLC) is a highly aggressive malignancy with very high mortality rate. A prominent part of this because these carcinomas are refractory to chemotherapies, such as etoposide or cisplatin, making effective treatment almost impossible. Here, we report that elevated expression the RAGE variant-V in SCLC promotes homology-directed DNA DSBs repair when challenged anti-cancer drugs. This variant exclusively localizes nucleus, interacts members double-strand break (DSB)...
Abstract Aim Novel dietary interventions focused on fasting, have gained scientific and public attention. Periodic fasting has emerged as a modification promoting beneficial effects metabolic syndrome. This study aimed to assess whether periodic reduces albuminuria in patients with type 2 diabetes diabetic nephropathy determine reduction relates activation of nephropathy-driven pathways. Methods Forty (HbA1c 7.8±0.2% [62.1±2.3 mmol/mol]) increased albumin-to-creatinine ratio (ACR) were...
Stable cell cloning is an essential aspect of biological research. All advanced genome editing tools rely heavily on stable, pure, single cell-derived clones genetically engineered cells. For years, researchers have depended single-cell dilutions seeded in 96- or 192-well plates, followed by microscopic exclusion the wells with more than without a cell. This method not just laborious, time-consuming, and uneconomical but also liable to unintentional error identifying these disadvantages may...
With the advancement of laser-based microscopy tools, it is now possible to explore mechano-kinetic processes occurring inside cell. Here, we describe advanced protocol for studying DNA repair kinetics in real time using laser induce damage. This can be used inducing, testing, and mechanisms associated with double-strand breaks, interstrand cross-link repair, single-strand break repair. For complete details on use execution this protocol, please refer Kumar et al. (2017, 2020).
of chemotherapy.The most frequently used agents are doxorubicin and cisplatinum.