RNA interference in mammalian cells is actively used to conduct genetic screens, identify and validate targets, elucidate regulators modifiers of cellular pathways. To ensure the specificity efficacy active 21mer siRNA molecules, it pertinent develop a strategy for their rational design. Here we show that most functional siRNAs have characteristic sequence features. We tested 601 targeting one exogenous three endogenous genes. The was determined at protein level. Using decision tree...

The purpose of this study was to evaluate trends in the sources dietary sodium intake a representative Korean population Korea National Health and Nutrition Examination Survey (KNHANES) conducted 1998, 2001, 2005, 2007, 2008, 2009, 2010. consisted 53,570 men women aged 7 years or older participating KNHANES during corresponding years. Overall mean percent contribution each dish food group across 1998-2010 were calculated. means ranged between 4,572.6-5,498.9 mg/d 1998-2010. major groups...

Norathyriol is a metabolite of mangiferin. Mangiferin has been reported to inhibit α -glucosidase. To the best our knowledge, no study conducted determine or compare those two compounds on inhibiting -glucosidase in vitro and vivo by far. In this study, we determined inhibitory activity norathyriol mangiferin evaluated their antidiabetic effect diabetic mice. The results showed that inhibited noncompetitive manner with an IC 50 value 3.12 μ M, which more potent than (IC = 358.54 M) positive...

The aim of this study was to examine calf circumference in relation cognitive frailty community-dwelling older adults. Cross-sectional analysis performed on the first-year baseline data 1559 adults aged 70–84 years enrolled Korean Frailty and Aging Cohort Study. final included 1221 who were non-dependent terms instrumental activities daily living, underwent function assessments. Physical defined using Fried Index. Cognitive impairment as a score 1.5 standard deviations below age-, sex-...

Background Sitosterolemia is an inherited lipid disorder which presents with elevated serum sitosterol and can result in increased risk of premature cardiovascular disease. However, cannot be accurately measured by routine diagnostic assays, meaning that sitosterolemia diagnosis often difficult, especially many clinical features overlapping familial hypercholesterolemia. With such complications resulting increasing reports misdiagnosis, the prevalence predicted to much higher than previously...

There is a growing body of evidence demonstrating an association between smoking and DNA methylation. Accordingly, methylation now considered promising biomarker exposure. We evaluated the relationship markers (AHRR F2RL3) urine cotinine as well self-reported status. levels AHRR F2RL3 in blood were measured 330 adults (46 to 87 years age). Pyrosequencing was performed measure associated with The lung cancer risk analyzed using logistic regression analysis. genes significantly hypomethylated...

In diabetic retinopathy (DR), neutrophil extracellular traps (NET) and kallikrein-kinin system are considered as contributing factors. However, the detail activation mechanisms has not been fully understood. Since NET could provide negative-charged surface for factor XII activated (XIIa) can initiate system, this study investigated whether patients with DR show of NET, system.The markers related to (DNA-histone complex) (high-molecular-weight kininogen, prekallikrein, bradykinin) XIIa were...

Accurate, precise and reliable laboratory test results play a critical role in medical decision making. To satisfy the increasing needs clinical tests, analyzers have been advanced. In this study, authors aimed to evaluate analytical performance of Alinity i system (Abbott Laboratories, IL, USA) for diverse analytes measured by using immunoassay principle.Analytical recently launched has evaluated 31 assays aspects precision, linearity measurement range, correlation with Architect i2000sr...

Dye-sensitized solar cells (DSSCs) were fabricated with closed- or open-ended freestanding TiO2 nanotube arrays as photoelectrodes that decorated carbon materials and large nanoparticles (NPs) to enhance energy conversion efficiency. The efficiency of DSSCs based on increased from 4.47% 5.39%, compared the closed-ended arrays. In arrays, 5.39% 6.19% due better electron transport, a scattering layer 6.24% more light harvesting without layer. Moreover, both 6.98%, which is an enhancement...

Telomere length (TL) is a prognostic indicator in Caucasian chronic lymphocytic leukemia (CLL), but its significance Asian CLL remains unknown. To investigate the of TL and correlation with cytogenetic aberrations somatic mutations, we analyzed measurements at cellular level by interphase fluorescence situ hybridization patients Korea. The present study enrolled 110 (41 females 69 males) diagnosed according to World Health Organization criteria (2001-2017). TLs bone marrow nucleated cells...

Abstract Background Although a diagnosis of infectious diseases is essential for timely treatment, the performance diagnostic tests has been hardly evaluated due to variable results that are influenced by multiple factors in different conditions. In present study, Alinity i system, which newly developed immunoassay diagnose diseases, was evaluated. Methods We precision, linearity, correlation, and carryover 16 analytes (HAV Ab IgG, HBsAg, HBeAg, anti‐HBc, anti‐HBe, anti‐HBs, anti‐HCV, HIV...

Masked signal modeling (MSM) has gained considerable attention in self-supervised learning. Considering that the existing masked strategies may cause model to damage structural or semantic understanding of key regions, we present an improved auto-encoder based on self-distillation and normal vector prediction can augment capacity recognize local regions. Firstly, given a point cloud with vector, our approach involves grouping embedding clouds lacking corresponding vector. Secondly,...

This study focused on improving the solubility of silodosin, a drug poorly soluble in water, by utilizing solid dispersions. Three types dispersions were examined and compared against powder: surface-attached (SA), solvent-wetted (SW), solvent-evaporated (SE). Polyvinyl alcohol (PVA) was identified as most effective polymer enhancing solubility. These prepared using spray-drying techniques with silodosin PVA polymer, employing solvents such ethanol, water-acetone mix. The physicochemical...

A linear gradient elution method using countercurrent chromatography was developed for the separation of four triterpenoid saponins from roots Pulsatilla koreana Nakai, including hederacolchiside E, which is responsible neuroprotective activity this plant. The target fraction obtained by 80% methanol solid phase column chromatography. partition coefficients compounds were very different, means they are difficult to separate with a single biphasic solvent system. Several important parameters...

Purpose: To compare the inter-strain differences of three rodent glaucoma models as induced by magnetic bead injection, hydrogel and circumlimbal suture. Methods: In Brown Norway (BN) Sprague Dawley (SD) rat strains, intraocular pressure (IOP) was elevated injection beads or to obstruct aqueous humor outflow external compression Maximum average IOP values were compared according both procedure strain over 1 month postoperatively. Retinal ganglion cell (RGC) density loss evaluated using...

The purpose of this study was to examine the association urinary sodium-to-creatinine ratio and potassium-to-creatinine with blood pressure in a cross-sectional comprising Korean adults who participated Healthy Twin Study. participants consisted 2653 men women Study aged ≥19 years. Participants’ excretion sodium, potassium, creatinine measured from overnight half-day urine samples. Food intake assessed using validated food frequency questionnaire. We examined systolic diastolic pressures...

Da Young Song, M.D., Joon Hee Lee, Sung Min Kim, M.T., Sun-Hee Jun, Kyunghoon Sang Hoon Junghan M.D.. Lab Med Online 2020;10:242-6. https://doi.org/10.3343/lmo.2020.10.3.242