A5091132895- Effects of Radiation Exposure
- Radiation Therapy and Dosimetry
- Rice Cultivation and Yield Improvement
- Radiation Dose and Imaging
- Agriculture, Soil, Plant Science
- Autophagy in Disease and Therapy
- Spaceflight effects on biology
- Alzheimer's disease research and treatments
- Childhood Cancer Survivors' Quality of Life
- Cancer-related Molecular Pathways
- Renal and related cancers
- Radioactivity and Radon Measurements
- GABA and Rice Research
- Biochemical effects in animals
- Neuroinflammation and Neurodegeneration Mechanisms
- Gut microbiota and health
- Plant responses to water stress
- Renal cell carcinoma treatment
- Crop Yield and Soil Fertility
- Estrogen and related hormone effects
- Pediatric health and respiratory diseases
- Kruppel-like factors research
- Agricultural Systems and Practices
- Traditional Chinese Medicine Studies
- Management of metastatic bone disease
National Institutes for Quantum Science and Technology
2013-2024
Ibaraki University
2014-2019
Ehime University
2012-2013
Juntendo University
2009-2010
Development Fund
2009
National Institute for Radiological Protection
2007
Tokyo Medical and Dental University
1972
The carcinogenicity of potassium bromate, a food additive and neutralizer in permanent waving, was tested by adding it to the drinking water F344 rats for 110 weeks. Groups 53 males females, each, were given solutions 500 or 250 ppm bromate distilled water. A concentration markedly inhibited an increase body weight male rats. mean survival time shortest (88.1 +/- 18.1 wk); times other groups 101-104 percentage week 104 relatively high all groups, 77.4% 66.0% females control group. High...
ABSTRACT Lipid droplets (LDs), which are ubiquitous organelles consisting of a neutral lipid core coated with phospholipid monolayer, play key roles in the regulation cellular metabolism. Although it is well known that mammalian oocytes and embryos contain LDs amount varies among animal species, their physiological functions remain unclear. In this study, we have developed method based on two-step centrifugation for efficient removal almost all from mouse MII (delipidation). We found...
Insoluble iron deposits often exist as oxide nanoparticles in protein aggregates, impaired ferritin, or activated microglia and have been implicated major causes of neuroinflammation Alzheimer's disease. However, no crucial evidence has reported to support the therapeutic effects current chelators on deposition various molecular forms insoluble iron. We investigated effect carbon ion stimulation (CIS) via a transmission beam deposits, inclusion bodies, associated biological response 5xFAD AD...
Lysosomes are acidic and highly dynamic organelles that essential for macromolecule degradation many other cellular functions. However, little is known about lysosomal function during early embryogenesis. Here, we found the number of lysosomes increased after fertilization. were abundant mouse preimplantation development until morula stage, but their numbers decreased slightly in blastocysts. Consistently, protein expression level mature cathepsins B D was high from one-cell to stages low...
Embryo quality is a critical parameter in assisted reproductive technologies. Although embryo can be evaluated morphologically, morphology does not correlate perfectly with viability. To improve this, it important to understand which molecular mechanisms are involved control. Autophagy an evolutionarily conserved catabolic process cytoplasmic materials sequestered by autophagosomes degraded lysosomes. We previously demonstrated that autophagy highly activated after fertilization and...
Effects of organic (Italian ryegrass and Bokashi) chemical fertilizer on growth, yield, grain quality rice (Oryza sativa L.) were compared under different planting densities in 2013/2014 2014/2015. Italian was incorporated into the soil as green manure. Bokashi (a mixture materials) applied basal dressing. To measure yield its components, 30 hills chosen for each treatment. Rice grains harvested from treatment to assess evaluate accumulation structures using a scanning electron microscope....
Renal toxicity is the principal health concern after uranium exposure. Children are particularly vulnerable to exposure; with contact depleted in war zones or groundwater contamination most likely exposure scenarios. To investigate renal sensitivity during development, we examined distribution and uranium-induced apoptosis kidneys of neonate (7-day-old), prepubertal (25-day-old) adult (70-day-old) male Wistar rats. Mean concentrations increased both age-at-exposure level subcutaneous...
Children are especially sensitive to ionizing radiation and chemical carcinogens, limiting their cancer risk is of great public concern. Calorie restriction (CR) a potent intervention for suppressing cancer. However, CR generally not appropriate children. This study, therefore, examined see if adult-onset influences the lifetime in mice after early-life exposure radiation. Infant male (1-week-old) were exposed 3.8 Gy X-rays, fed control 95 kcal/week or 65 diet from 7 weeks age (adult stage),...
As a space project, in "Stem Cells" by the Japan Aerospace Exploration Agency (JAXA), frozen mouse ES cells were stored on International Space Station (ISS) Minus Eighty Degree Laboratory Freezer for ISS (MELFI) 1584 days. After taking these back to ground, thawed and cultured, their gene expressions comprehensively analyzed using RNA sequencing order elucidate early response of long-time exposure radiation consisting various ionized particles. The comparisons expression involved...
The relative biological effectiveness (RBE) of neutrons depends on their physical nature (e.g., energy) and the context end points, materials). From perspective radiological protection, age is an important that influences radiation-related cancer risk, but very few studies have addressed its potential impact neutron effects. We therefore investigated influence effect accelerator-generated fast (mean energy, ∼2 MeV) in animal model breast carcinogenesis. Female Sprague-Dawley rats at 1, 3 7...
Women who are heterozygous for deleterious BRCA1 germline mutations harbor a high risk of hereditary breast cancer. Previous Brca1-heterozygous animal models do not recapitulate the cancer phenotype, and thus all currently used knockout adopt conditional, mammary-specific homozygous Brca1 loss or addition Trp53 deficiency. Herein, we report creation characterization novel mutant rat model harboring L63X mutation, which mimics founder mutation in Japan, through CRISPR-Cas9-based genome...
Accurate cancer risk assessment of low-dose radiation poses many challenges that are partly due to the inability distinguish radiation-induced tumors from spontaneous ones. To elucidate characteristic features tumors, we analyzed 163 medulloblastomas developed either spontaneously or after X-ray irradiation at doses 0.05-3 Gy in Ptch1 heterozygous mice. All showed loss heterozygosity broad regions on chromosome 13, with losses all consecutive markers distal locus (S-type). In contrast, 3...
Genetic, physiological and environmental factors are implicated in colorectal carcinogenesis. Mutations the mutL homolog 1 (MLH1) gene, one of DNA mismatch repair genes, a main cause hereditary colon cancer syndromes such as Lynch syndrome. Long-term chronic inflammation is also key risk factor, responsible for colitis-associated cancer; radiation exposure known to increase risk. Here, we studied effects on inflammation-induced carcinogenesis repair-proficient repair-deficient mice. Male...
Although the kidneys comprise a critical target of uranium exposure, dynamics renal distribution have remained obscure. Uranium is considered to function physiologically in form uranyl ions that high affinity for phosphate groups. The present study applied microbeam-based elemental analysis precisely determine phosphorus and male Wistar rats exposed uranium. One day after single subcutaneous injection acetate (2 mg/kg), areas concentrated were scattered S3 segments proximal tubule kidneys,...
Although the risk of breast cancer after high-dose-rate irradiation has been firmly established, however, incurred for low-dose-rate is not well understood. Here we provide experimental evidence dose rate and age dependencies induced by continuous γ-ray on mammary carcinogenesis. Female rats received whole-body at one following time points: 7 weeks (denoted adults) a 3–60 mGy/h (4 Gy total); or either 3 juveniles) 6 (1–8 total). Additional were acutely irradiated 13 30 Gy/h (0.5–4 We...
Ionizing radiation can damage DNA and, therefore, is a risk factor for cancer. Eker rats, which carry heterozygous germline mutation in the tumor-suppressor gene tuberous sclerosis complex 2 (Tsc2), are susceptible to radiation-induced renal carcinogenesis. However, molecular mechanisms involved Tsc2 inactivation unclear. We subjected Fischer 344 × (Long Evans
Exposure to carcinogens early in life may contribute cancer development later life. The amount of radiation exposure children experience during medical procedures has been increasing, so it is important evaluate the risk developing organs. Toward this goal, we assessed renal cell carcinoma using Eker rats as a kidney tumor model. F1 hybrids male (Tsc2 mutant) and female F344 were irradiated with 0.5 or 2 Gy gamma on gestation days 15 19, postnatal 5, 20, 49. At 27 weeks age, kidneys examined...
Abstract Radiation exposure during the peri-pubertal period is a proven risk factor for breast cancer, whereas parity an established protective factor. The present study investigated whether imposes differential effects against radiation-induced rat mammary carcinoma depending on age at exposure. Pre- and post-pubertal female rats, irradiated or left unirradiated, were mated allowed to nurse until weaning unmated. Appearance of tumors was monitored, serum concentrations estradiol...