A5091653202- Escherichia coli research studies
- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Listeria monocytogenes in Food Safety
- Meat and Animal Product Quality
- Microbial infections and disease research
- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Pharmaceutical and Antibiotic Environmental Impacts
- Advanced Chemical Sensor Technologies
- Bacterial Genetics and Biotechnology
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Biosensors and Analytical Detection
- Microbial bioremediation and biosurfactants
- Biochemical Analysis and Sensing Techniques
- Transgenic Plants and Applications
- Bee Products Chemical Analysis
- Food Supply Chain Traceability
- Microbial Inactivation Methods
- Seedling growth and survival studies
- Plant Pathogenic Bacteria Studies
- Probiotics and Fermented Foods
- Geotechnical Engineering and Soil Stabilization
- Ecology and Conservation Studies
Agriculture and Food
2016-2022
Commonwealth Scientific and Industrial Research Organisation
2013-2022
Animal, Food and Health Sciences
2013-2020
Health Sciences and Nutrition
2011
ABSTRACT Shiga toxigenic Escherichia coli O157 is the leading cause of hemolytic uremic syndrome (HUS) worldwide. The frequencies stx genotypes and incidences O157-related illness HUS vary significantly between Argentina Australia. Locus-specific polymorphism analysis revealed that lineage I/II (LI/II) E. isolates were most prevalent in (90%) Australia (88%). Argentinean LI/II shown to belong clades 4 (28%) 8 (72%), while Australian identified as 6 (15%), 7 (83%), (2%). Clade was associated...
ABSTRACT Escherichia coli O157 is a food-borne pathogen whose major reservoir has been identified as cattle. Recent genetic information indicated that populations of E. from cattle and humans can differ genetically this variation may have an impact on their ability to cause severe human disease. In addition, there emerging evidence strains different geographical regions also be divergent. To investigate the extent variation, we used Shiga toxin bacteriophage insertion sites (SBI),...
Shiga toxin-producing Escherchia coli (STEC) O157:H7 is a zoonotic pathogen that causes numerous food and waterborne disease outbreaks. It globally distributed, but its origin the temporal sequence of geographical spread are unknown.We analyzed whole-genome sequencing data 757 isolates from 4 continents, performed pan-genome analysis to identify core genome and, this, extracted single-nucleotide polymorphisms. A timed phylogeographic was on subset investigate worldwide spread.The common...
ABSTRACT While the differential association of Escherichia coli O157 genotypes with animal and human hosts has recently been well documented, little is known about their distribution between countries how this might affect regional disease rates. Here, we used a 48-plex single nucleotide polymorphism (SNP) assay to segregate 148 E. isolates from Australia, Argentina, United States into 11 SNP lineages. We also investigated relationship lineages, Shiga toxin (Stx) gene profiles, total Stx...
Beef cattle are known reservoirs of Escherichia coli O157; therefore, it is possible that they may be for other enterohemorrhagic E. (EHEC) serotypes. This study investigated the prevalence EHEC serotypes O26, O45, O91, O103, O111, O121, O145, and O157 in 300 beef fecal samples.A combination (140 grain-fed 160 grass-fed) samples were collected post-evisceration from Australian abattoirs. Enriched tested putative virulence markers stx₁, stx₂, eae using real-time PCR. Samples positive stx then...
Antimicrobial agents are used in cattle production systems for the prevention and control of bacterial associated diseases. A consequence their use is potential development antimicrobial resistance (AMR). Enterococcus faecium faecalis that resistant to antimicrobials increased concern public health officials throughout world as they may compromise ability various treatment regimens disease infection human medicine. Australia a major exporter beef; however it does not have an ongoing...
ABSTRACT The ability of Escherichia coli and Salmonella isolates to attach Caco-2 HT-29 cell monolayers was measured. All displayed a greater cells than cells, overall E. attached better both lines isolates. Bacteria that were considered be pathogenic no those not pathogenic. Additionally, correlation found between line attachment previously determined hydrophobicity results.
A survey was conducted to determine the relative prevalence of Salmonella serovars on whole chicken carcasses before and after processing in 3 Australian poultry abattoirs. Ninety 180 were tested for processing, respectively. Each carcass subjected a buffered peptone water rinse according Standard methodologies determined using methodologies. After isolation, isolates serotyped results analyzed percentage each serovar at both points. Sofia shown significantly increase its (P < or = 0.05)...
The aims of this work were to determine the distribution and concentration Escherichia coli O157 in lots beef destined for grinding (manufacturing beef) that failed meet Australian requirements export, use these data better understand performance sampling plans based on binomial distribution, consider alternative approaches evaluating plans. For each five from which E. had been detected, 900 samples external carcass surface tested. was not detected three lots, whereas two 2 74 samples....
Australia relies on periodic antimicrobial resistance (AMR) surveys to determine trends and changes in AMR animal production systems. This study is a follow-up survey of Escherichia coli from healthy cattle at slaughter conducted 2013, which provided baseline data prevalence across groups practices. In this study, 591 beef cattle, 194 dairy 216 veal calf fecal samples were collected 25 processing establishments Australia, representing approximately 77% total export volume. A 969...
Shockwaves generate instantaneous high pressures, which could affect meat shelf-life or quality. This study assessed microbiological counts, pH, drip, cook and moisture loss texture of striploin (longissimus lumborum) brisket (pectoralis profundus) treated with electrical shockwave (25 kV, 8 pulses) subsequently stored (−0.5 °C) for 0, 4, 8, 12, 16 20 weeks. Shockwave did not total viable counts (p>0.05), all samples considered microbiologically acceptable (<7 log10 CFU/cm2) after reduced...
Shiga toxin-producing Escherichia coli (STEC) is a foodborne pathogen, and serotype O157:H7 typically associated with severe disease. Australian STEC epidemiology differs from many other countries, as outbreaks HUS cases appear to be more often non-O157 serogroups. It not known why strains of O157 might differ in virulence international strains. Here we investigate the reduced Multiple genetic analyses were performed, including SNP-typing, compare core genomes isolates, accessory genome...
Abstract Enterococci are opportunistic, potentially life-threatening pathogens of humans that difficult to manage due antimicrobial resistance. Historically, enterococci entering the food-chain through livestock have been viewed as a likely source resistance in humans. Australian human-derived clinical propensity be resistant multiple classes antimicrobials including vancomycin. Recent studies involving pigs and chicken virtually excluded these species reservoirs infection for However,...
This article contains temperature logger datasets obtained for refrigerated cartons of Australian vacuum packaged chilled beef stored under near ideal conditions (∼- 1 °C) in Australia (CONTROL) and shipped via land sea to three destinations China (China-1, China-2, China-3) described detail previously [1]. Cartons were 84, 98, 120 140 days postslaughter. Temperature data acquired during shipping storage using i-buttons (Thermocron-TCS, Baulkham Hills, Australia) LogTags (TRIX-16, LogTag...