A5089764375- Nanoparticles: synthesis and applications
- Inhalation and Respiratory Drug Delivery
- Effects and risks of endocrine disrupting chemicals
- 3D Printing in Biomedical Research
- Microplastics and Plastic Pollution
- Air Quality and Health Impacts
- Nanotechnology research and applications
- Nanoparticle-Based Drug Delivery
- Animal testing and alternatives
- Chemical Safety and Risk Management
- Carbon Nanotubes in Composites
- Magnetic and Electromagnetic Effects
- Pediatric health and respiratory diseases
- Electromagnetic wave absorption materials
- Smart Materials for Construction
- Graphene and Nanomaterials Applications
- Microbial Community Ecology and Physiology
- Electrical and Bioimpedance Tomography
- Advanced Chemical Sensor Technologies
- NF-κB Signaling Pathways
- Tissue Engineering and Regenerative Medicine
- Infant Nutrition and Health
- Cystic Fibrosis Research Advances
- Pickering emulsions and particle stabilization
- Immune Response and Inflammation
Arizona State University
2016
Wright-Patterson Air Force Base
2013-2015
Wright State University
2007-2015
United States Air Force Research Laboratory
2013
Engineered nanomaterials (ENMs) are increasingly entering the environment with uncertain consequences including potential ecological effects.Various research communities view differently whether ecotoxicological testing of ENMs should be conducted using environmentally relevant concentrations-where observing outcomes is difficult-versus higher ENM doses, where responses observable.What exposure conditions typically used in assessing hazards to populations?What test ecosystem-scale...
Expansion in production and commercial use of nanomaterials increases the potential human exposure during lifecycle these materials (production, use, disposal). Inhalation is a primary route to nanomaterials; therefore it critical assess their respiratory hazard. Herein, we developed three-dimensional alveolar model (EpiAlveolar) consisting epithelial cells, fibroblasts, endothelial with or without macrophages for predicting long-term responses aerosols. Following thorough characterization...
The increasing use of multi-walled carbon nanotubes (MWCNTs) in consumer products and their potential to induce adverse lung effects following inhalation has lead much interest better understanding the hazard associated with these nanomaterials (NMs). While current regulatory requirement for substances concern, such as MWCNTs, many jurisdictions is a 90-day rodent test, monetary, ethical, scientific concerns this test led an international expert group convene Washington, DC, USA, discuss...
Gold nanoparticles (Au-NPs) have been designated as superior tools for biological applications owing to their characteristic surface plasmon absorption/scattering and amperometric (electron transfer) properties, in conjunction with low or no immediate toxicity towards systems. Many studies shown the ease of designing application-based using Au-NPs but interaction this nanosized material biomolecules a physiological environment is an area requiring deeper investigation. Immune cells such...
In the field of toxicology nanomaterials, scientists have not clearly determined if observed toxicological events are due to nanoparticles (NPs) themselves or dissolution ions released into biophysiological environment both phenomenon participate in combination based upon their bioregional and temporal occurrence during exposure conditions. Consequently, research involving analysis silver NPs (Ag-NPs) has shifted towards assessment 'nanosized' comparison its solvated 'ionic' counterpart....
Scientists are using in vitro methods to answer important research questions and implementing strategies maximize the reliability human relevance of these methods. One strategy is replace use fetal bovine serum (FBS)-an undefined variable mixture biomolecules-in cell culture media with chemically defined or xeno-free medium. In this study, A549 cells, a lung alveolar-like line commonly used respiratory research, were transitioned from medium containing FBS without FBS. A successful...
Inhalation is a major route by which human exposure to substances can occur. Resources have therefore been dedicated optimize human-relevant in vitro approaches that accurately and efficiently predict the toxicity of inhaled chemicals for robust risk assessment management. In this study-the IN Systems PredIct REspiratory Initiative-2 cell-based systems were used ability cause portal-of-entry effects on respiratory tract. A bronchial epithelial cell line (BEAS-2B) reconstructed tissue model...
Adverse outcome pathways (AOPs) help to organize available mechanistic information related an adverse into key events (KEs) spanning all organizational levels of a biological system(s). AOPs, therefore, aid in the understanding particular pathogenesis and also with linking exposures eventual toxic effects. In regulatory context, knowledge disease mechanisms can design testing strategies using vitro methods that measure or predict KEs relevant effect interest. The AOP described here evaluates...
In vitro inhalation toxicology methods are increasingly being used for research and regulatory purposes. Although the opportunity increased human relevance of in compared to vivo tests has been established discussed, how systematically account variability maximize reliability these methods, especially assays that use cells cultured at an air–liquid interface (ALI), received less attention. One tool evaluate robustness test is cause-and-effect (C&E) analysis, a conceptual approach analyze key...
The overt hazard of carbon nanotubes (CNTs) is often assessed using in vitro methods, but determining a dose-response relationship still challenge due to the analytical difficulty quantifying dose delivered cells. An approach accurately quantify CNT doses for submerged adherent cell culture systems UV-VIS-near-infrared (NIR) spectroscopy provided here. Two types multi-walled CNTs (MWCNTs), Mitsui-7 and Nanocyl, which are dispersed protein rich media, studied as tested materials. Post 48 h...
Traditional in vitro toxicity experiments typically involve exposure of a mono- or co-culture cells to nanoparticles (NPs) static conditions with the assumption 100% deposition (i.e. dose) well-dispersed particles. However, cellular dose can be affected by agglomeration and unique transport kinetics NPs biological media. We hypothesize that shear flow address these issues achieve more predictable dosage. Here, we compare behavior gold diameters 5, 10 30 nm dynamic models. also utilize...
This article presents a multistage framework for evaluating the strength of evidence nanomaterial (NM) exposure characterization data to optimize utility in vitro testing strategies human health risk assessment. is intended aid assessors relevance from tests and development new strategies. The initial stage frames scenarios interest advance incorporate aspects such as release points, route exposure, biological environmental transformations, dose metrics, targets subsequent stages. second...
A broad range of in vitro test methods have been developed given their numerous potential advantages over vivo tests. We describe here key resources and tools to increase the reliability reproducibility toxicological methods.
RETURN TO ISSUEPREVAdditions and Correc...Additions CorrectionsNEXTORIGINAL ARTICLEThis notice is a correctionCorrection to Use of EpiAlveolar Lung Model Predict Fibrotic Potential Multiwalled Carbon NanotubesHana BarosovaHana BarosovaMore by Hana Barosova, Anna G. MaioneAnna MaioneMore Maione, Dedy SeptiadiDedy SeptiadiMore Septiadihttp://orcid.org/0000-0003-2353-7508, Monita SharmaMonita SharmaMore Sharma, Laetitia HaeniLaetitia HaeniMore Haeni, Sandor BalogSandor BalogMore...