A5100767399- Reproductive System and Pregnancy
- Psoriasis: Treatment and Pathogenesis
- Receptor Mechanisms and Signaling
- Immune Cell Function and Interaction
- T-cell and Retrovirus Studies
- Aquaculture disease management and microbiota
- Cancer-related molecular mechanisms research
- Child Abuse and Trauma
- Maternal Mental Health During Pregnancy and Postpartum
- Neuroscience and Music Perception
- Autoimmune and Inflammatory Disorders Research
- Endometriosis Research and Treatment
- Biochemical and Structural Characterization
- Circular RNAs in diseases
- HIV Research and Treatment
- Reproductive tract infections research
- Dialysis and Renal Disease Management
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Fractional Differential Equations Solutions
- Kidney Stones and Urolithiasis Treatments
- Immunotherapy and Immune Responses
- Asthma and respiratory diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Plant-based Medicinal Research
- Protein Hydrolysis and Bioactive Peptides
Xinxiang Medical University
2023
First Affiliated Hospital of Xinxiang Medical University
2016-2023
Pudong Medical Center
2016-2022
Fudan University
2010-2022
Huangshi Central Hospital
2017
Hubei Polytechnic University
2013-2017
Shanghai Medical College of Fudan University
2012
Huadong Hospital
2010-2012
Huashan Hospital
2010
We surveyed the group B Streptococcus ( GBS ) strains isolated from four teaching hospitals during 1‐year period to investigate current serotypes and antimicrobial resistance status of these strains. A total 231 non‐duplicate colonizing isolates were collected pregnant women. Antimicrobial susceptibility was tested by disk diffusion method. Serotype performed a multiplex polymerase chain reaction PCR Analysis mechanisms amplification DNA sequencing. Seven (Ia, Ib, II , III V, VI VIII...
Signal transducer and activator of transcription (STAT) is a unique protein family that binds to DNA plays vital role in regulating major physiological cellular processes. Seven STAT genes have been identified the human genome. Several studies suggest members be involved cancer development, progression, metastasis. However, predictive relationship between expression immune cell infiltration endometrial remains unknown.We explored prognosis using various databases. The STRING, GeneMANIA,...
Background. Ferroptosis is a recently described form of intentional cellular damage that iron-dependent and separate from apoptosis, necrosis, autophagy. It has been demonstrated to be adequately regulated by long noncoding RNAs (lncRNAs) in various cancers. However, the predictive profile ferroptosis-related lncRNAs (FRLs) endometrial carcinoma (EC) unknown. Herein, FRLs associated with uterine corpus (UCEC) prognosis were screened predict treatment response EC. Methods. Samples EC adjacent...
BACKGROUND:The aim of this study was to investigate the antimicrobial property peptide LL-37 sequences. MATERIAL AND METHODS:Humanized antibacterial and mutant were prepared by chemical synthesis. The physicochemical properties analyzed SWISS-MODEL online prediction tool. Molecular docking between fragments palmitoyl transferase PagP made with Lamarckian genetic algorithm AutoDock1.5.6. RESULTS:The systems contacted each other at 8.75 picosec. After 20 picsec, system had no trend...
To determine whether the neurokinin-1 receptor (NK1R) plays a role in activation of RBL-2H3 mast cells after FcεRΙ aggregation.NK1R expression was inhibited by small hairpin RNA (shRNA) against NK1R, and determined western blotting. For activation, both NK1R knockdown control were sensitized dinitrophenol (DNP)-specific IgE stimulated with antigen DNP-bovine serum albumin (BSA). Following cells, monocyte chemoattractant protein (MCP-1) production intracellular calcium flux monitored ELISA...
Plasminogen activator inhibitor-1 (PAI-1) is the most effective protease inhibitor in fibrinolysis system, and plays an important role remodeling of extracellular matrix. We therefore explored whether PAI-1 involved change lung structure with increasing age. gene knockout mice wild-type were sacrificed at age 3 weeks, months, 6 months 15 for histopathology analysis, assessed relationship between Six-month-old chosen further studies. Elastin was detected using Weigert staining. measured...
Objective. To investigate the profiles of vaginal microbiome in patients with endometrial hyperplasia and to explore potential value diagnosis hyperplasia. Materials/Methods. 26 suffering from abnormal uterine bleeding (AUB) thickened endometrium revealed by transvaginal ultrasonography were enrolled. Based on pathology, 12 classified as Veh group 14 proliferative Vne group. The samples collected for presence microbial DNA high-throughput next-generation sequencing 16S rRNA gene. α-diversity...
目的检验成人心理弹性量表(Resilience Scale for Adults, RSA)在城市孕妇人群中的信效度。 方法以2016年6月至2017年8月期间在上海两所接产医院建大卡并愿意参加“上海亲子队列”的1 689名孕妇为研究对象, 孕16周左右填写RSA, 孕32~36周时填写焦虑和抑郁量表(SAS和CES-D)。在对RSA进行项目分析并探索量表结构的基础上, 对修订后的量表进行信效度检验。 结果经项目分析和探索性因子分析, 修订后的RSA保留22个条目, 含5个因子, 因子负荷在0.428~0.768, 累积方差解释率为52.493%。总量表的分半信度为0.798, 总量表及各因子的内部一致性系数为0.546~0.850。各因子分与总量表分高度相关, 相关系数为0.540~0.778;各因子间相关系数略低, 为0.171~0.590。修订后的量表总分与孕晚期焦虑、抑郁水平呈显著负相关。验证性因子分析发现, χ2/df值为6.783, GFI、CFI、IFI、AGFI值分别为0.919、0.881、0.881、0.897, RMSEA值为0.059。...
Objective To evaluate the effect of epigallocatechin gallate (EGCG) on T helper cell 1 (Th1) and Th2 in psoriasis patients. Methods A total 33 patients with plaque-type vulgaris were enrolled, peripheral blood mononuclear cells (PBMC) isolated cultured. The appropriate concentration EGCG was determined by methyl thiazol tetrazolium (MTT) assay. PBMC at exponential growth phase divided into 2 groups to be treated (EGCG group) or not (control for 24 hours. Flow cytometry performed...
Objective To establish and assess a new screening method for anti-HIV-1 drugs. Methods JLTRG cells were co-cultured with different proportions of H9/HTLV-ⅢB 24, 48, 72 96 h. Intensity density green fluorescent protein observed under fluorescence microscope, tested using flow cytometry. The optimal proportion in co-culture system the culture time determined. effectiveness Enfuvirtide (T20) Efavirenz (EFV), their half maximal inhibitory concentrations (IC50) determined by cell life...
Objective To evaluate the protective effect of epigallocatechin gallate (EGCG) against interleukin (IL) -17-induced injury to keratinocytes, and explore its mechanism. Methods Some cultured HaCaT cells were divided into 3 groups be treated with IL-17 alone at concentrations 50, 70, 90 μg/L, respectively, those receiving no treatment as blank control group. Some 5 groups: group μg/L alone, IL-17+EGCG 60 μmol/L EGCG, IL-17+SP600125 SP600125 (a JAK signaling pathway inhibitor) , IL-17+...
Objective To evaluate effects of ursolic acid (UA)on interleukin-33 (IL-33)expression in HaCaT cells induced by interferon-γ (IFN-γ), and to explore their mechanism. Methods Some were treated with UA at different concentrations (0, 0.1, 1, 5, 10, 20, 40 80 μmol/L)for 24, 48 72 hours separately. Then, methyl thiazolyl tetrazolium (MTT)assay was conducted cell proliferative activity. A model inflammation established culture the presence 200 μg/L IFN-γ. Some classified into several...
目的探索5-羟色胺1A（5-HT1A）受体激动剂8-OH-DPAT对青春期大鼠病理性攻击行为及对前额叶皮质、海马内脑源性神经营养因子（BDNF）和胶质细胞源性神经营养因子（GDNF）的影响。 方法出生后21 d的雄性SD大鼠42只随机分为6组（n=7）：模型组、正常组、模型+药物组、模型+生理盐水（NS）组、正常+药物组、正常+NS组。模型组、模型+药物组及模型+NS组采用早年慢性应激建立病理性攻击模型，余3组正常饲养。建模成功后对模型+药物组、正常+药物组及模型+NS组、正常+NS组分别行2周的8-OH-DPAT（0.5 mg/kg）或生理盐水（2 mL/只）腹腔注射。用居住-入侵实验检测大鼠攻击行为，蛋白质印迹分析检测大鼠脑前额叶皮质及海马内BDNF、GDNF蛋白表达水平。 结果与正常组比较，模型组攻击潜伏期缩短（P 结论前额叶皮质及海马内BDNF、GDNF可能与早年慢性应激所致青春期大鼠病理性攻击行为的发生有关。5-HT1A受体激动剂可上调前额叶皮质及海马内BDNF、GDNF的蛋白表达，并在一定程度上减轻早年慢性应激所致青春期大鼠病理性攻击行为。
目的探索突触后致密物-95(postsynaptic density-95, PSD-95)信号通路是否参与并介导5-HT1A受体激动剂对大鼠病理性攻击行为的改善作用及调控机制。 方法将成功构建的24只病理性攻击大鼠按照抽签法分为4组，分别为8-OH-DPAT组、8-OH-DPAT+ZL006组、ZL006组、NaCl组, 5-HT1A受体激动剂(8-OH-DPAT)以1 mg/kg每日腹腔注射并持续2周、PSD-95阻断剂(ZL006)以1 mg/kg每隔3天腹腔注射并持续2周。分别在给药前、给药1周后及给药2周后用居住-入侵实验测试大鼠病理性攻击行为变化，且每次居住-入侵实验前取大鼠眼眶后静脉血。取前额叶皮层、大鼠海马及下丘脑组织，用Western blot检测各脑区五羟色胺1A受体(serotonin 1A receptor, 5-HT1AR)、PSD-95及糖皮质激素受体(glucocorticoid GR)的表达，并采用ELISA试剂盒检测血清糖皮质激素含量。 结果8-OH-DPAT组大鼠攻击总数、攻击持续时间及攻击要害部位比在给药1周及2周后均有明显下降(P...