A5103272783- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Immunotherapy and Immune Responses
- Transgenic Plants and Applications
- Escherichia coli research studies
- Tryptophan and brain disorders
- IL-33, ST2, and ILC Pathways
- Pediatric health and respiratory diseases
- Stress Responses and Cortisol
- Cell Adhesion Molecules Research
- Immunodeficiency and Autoimmune Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Urban and spatial planning
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Digestive system and related health
- Gastrointestinal motility and disorders
- Infant Nutrition and Health
- Inflammatory mediators and NSAID effects
- Neuroendocrine Tumor Research Advances
- Angiogenesis and VEGF in Cancer
- Bone and Dental Protein Studies
- Sulfur Compounds in Biology
- Glutathione Transferases and Polymorphisms
RIKEN Center for Integrative Medical Sciences
2006-2021
Kyoto University
1985-2018
RIKEN
2009-2011
Hyogo Medical University
2010
Hyogo University
2002
Osaka University
1999
The mechanism to maintain homeostasis of the gut microbiota remains largely unknown despite its critical role in body defense. In intestines mice with deficiency activation-induced cytidine deaminase (AID), absence hypermutated IgA is partially compensated for by presence large amounts unmutated IgM and normal expression levels defensins angiogenins. We show here a predominant persistent expansion segmented filamentous bacteria throughout small intestine AID(-/-) mice. Reconstitution lamina...
Activation-induced cytidine deaminase (AID) plays an essential role in class switch recombination (CSR) and somatic hypermutation (SHM) of immunoglobulin genes. We report here that deficiency AID results the development hyperplasia isolated lymphoid follicles (ILFs) associated with a 100-fold expansion anaerobic flora small intestine. Reduction bacterial by antibiotic treatment AID-/- mice abolished ILF as well germinal center enlargement seen secondary tissues. Because inability to A on its...
Most of the immunoglobulin A (IgA) in gut is generated by B cells germinal centers Peyer's patches through a process that requires presence CD4+ follicular helper T(TFH) cells. The nature these T(FH) has been elusive. Here, we demonstrate suppressive Foxp3+CD4+ T can differentiate into TFH mouse patches. conversion Foxp3+ loss Foxp3 expression and subsequent interaction with Thus, environmental cues present promote selective differentiation distinct cell subsets, such as
Immunoglobulin A (IgA) is essential to maintain the symbiotic balance between gut bacterial communities and host immune system. Here we provide evidence that inhibitory co-receptor programmed cell death-1 (PD-1) regulates microbiota through appropriate selection of IgA plasma repertoires. PD-1 deficiency generates an excess number T follicular helper (T(FH)) cells with altered phenotypes, which results in dysregulated precursor germinal center Peyer's patches. Consequently, IgAs produced...
Immunoglobulin A (IgA) promotes health by regulating the composition and function of gut microbiota, but molecular requirements for such homeostatic IgA remain unknown. We found that a heavily glycosylated monoclonal recognizing ovalbumin coats Bacteroides thetaiotaomicron (B. theta), prominent symbiont phylum Bacteroidetes. In vivo, alters expression polysaccharide utilization loci (PUL), including functionally uncharacterized family provisionally named Mucus-Associated Functional Factor...
Abstract Small, soluble metabolites not only are essential intermediates in intracellular biochemical processes, but can also influence neighbouring cells when released into the extracellular milieu 1–3 . Here we identify metabolite and neurotransmitter GABA as a candidate signalling molecule synthesized secreted by activated B plasma cells. We show that cell-derived promotes monocyte differentiation anti-inflammatory macrophages secrete interleukin-10 inhibit CD8 + T cell killer function....
Peritoneal B1 cells are known to generate large amounts of antibodies outside their residential site. These play an important role in the early defense against bacteria and viruses, before establishment adaptive immune responses. Although many stimuli, including antigen, lipopolysaccharide, or cytokines, have been shown activate induce differentiation into plasma cells, molecular signals required for egress from peritoneal cavity not understood. We demonstrate here that direct through...
Activation-induced cytidine deaminase (AID), produced by the Aicda gene, is essential for immunoglobulin gene (Ig) alterations that form immune memory. Using a Cre-mediated genetic system, we unexpectedly found CD4+ T cells had expressed (exAID cells) as well B cells. ExAID increased with age, reaching up to 25% of and B220+ cell populations. remained IgM+, suggesting class-switched memory do not accumulate in spleen. In cells, AID was subset IFN-γ IL-10 but little IL-4 or IL-17, showed no...
B1 cells represent a distinct subset of B that produce most the natural serum IgM and much gut IgA function as an important component early immune responses to pathogens. The development depends on nuclear factor activated T c1 (NFATc1), transcription abundantly expressed by but not conventional B2 cells. However, factors regulate expression NFATc1 in remain unknown. Here we show vitamin A-deficient diet results reduction almost complete loss cell compartment. As consequence, mice have...
Intestinal IgA+ B cells are generated from IgM+ by in situ class switching two separate gut microenvironments: organized follicular structures and lamina propria (LP). However, the origin of LP is unknown. Transfer experiments to reconstitute IgA plasma aly/aly mice, which defective all because an NF-kappaB-inducing kinase (NIK) mutation, revealed that naive can directly migrate LP. This migration requires NIK-dependent activation stromal cells. By contrast, entry gut-primed independent with...
Macrophage-derived interleukin-12 (IL-12) is essential for the activation of a protective immune response against intracellular pathogens. In this study, we examined regulation IL-12 mRNA expression by monocyte-derived macrophages (MDM) in to Mycobacterium bovis BCG stimulation. A reverse transcription-PCR assay detected p40 at 3 h and showed peak 6 12 with subsequent decline. Semiquantitation levels competitive PCR revealed that pretreatment gamma interferon (IFN-gamma) amplified...