ABSTRACT The specific biofilm formation (SBF) assay, a technique based on crystal violet staining, was developed to locate plant essential oils and their components that affect formation. SBF analysis determined cinnamon, cassia, citronella differentially affected growth-normalized by Escherichia coli . Examination of the corresponding oil principal assay revealed cinnamaldehyde decreased compared biofilms grown in Luria-Bertani broth, eugenol did not result change, citronellol increased...

Journal Article Subinhibitory concentrations of cinnamaldehyde interfere with quorum sensing Get access C. Niu, Niu Department Biology, Georgia State University, Atlanta, GA 30303 USA Search for other works by this author on: Oxford Academic Google Scholar S. Afre, Afre E.S. Gilbert Gilbert, USA. E‐mail: esgilbert@gsu.edu Letters in Applied Microbiology, Volume 43, Issue 5, 1 November 2006, Pages 489–494, https://doi.org/10.1111/j.1472-765X.2006.02001.x Published: 01 2006 history Received:...

Plant compounds that induced Arthrobacter sp. strain B1B to cometabolize polychlorinated biphenyls (PCBs) were identified by a screening assay based on the formation of 4,4'-dichlorobiphenyl ring fission product. A chemical component spearmint (Mentha spicata), l-carvone, Aroclor 1242, resulting in significant degradation 26 peaks mixture, including selected tetra- and pentachlorobiphenyls. Evidence for PCB biodegradation included peak disappearance, phenylhexdienoate product, chlorobenzoate...

ABSTRACT There is limited knowledge of interspecies interactions in biofilm communities. In this study, Pseudomonas sp. strain GJ1, a 2-chloroethanol (2-CE)-degrading organism, and putida DMP1, p -cresol-degrading produced distinct biofilms response to model mixed waste streams composed 2-CE various -cresol concentrations. The two organisms maintained commensal relationship, with DMP1 mitigating the inhibitory effects on GJ1. A triple-labeling technique compatible confocal microscopy was...

Escherichia coli produces biofilms in response to the small molecule autoinducer-2 (AI-2), a product of LuxS enzyme. is part activated methyl cycle and could also affect biofilm development by AI-2-independent effects on metabolism. A luxS deletion mutant E. W3110 an inducible plasmid-luxS-complemented strain were used identify phenotypes. Differential interference contrast microscopy revealed distinct surface colonization patterns. Confocal followed quantitative image analysis determined...

Fourier transform infrared (FTIR) spectroscopy can detect biomolecular changes in bacterial cells response to drugs or other stimuli. To fully develop that area requires an understanding of IR spectral associated with the growth unperturbed cells. Such is still lacking, however. address this issue, attenuated total reflectance (ATR) FTIR has been used probe composition Staphylococcus aureus ATCC 6538 during exponential growth, 30-minute time resolution. We find prominent proteins, nucleic...

Fourier-transform infrared (FTIR) spectroscopy can detect biomolecular changes in bacterial cells response to drugs or other stimuli. Fully developing this area requires an understanding of IR spectral associated with the growth unperturbed cells. Such is still lacking, however. To address issue, attenuated total reflectance (ATR) FTIR has been used probe composition Staphylococcus aureus ATCC 6538 during exponential growth, a 30 min time resolution. We find prominent proteins, nucleic...

Biofilm structural heterogeneity affects a broad range of microbially catalysed processes. Solute transport limitation and autoinhibitor production, two factors that contribute to heterogeneous biofilm development, were investigated using BacMIST, computer simulation model. BacMIST combines cellular automaton algorithm for growth with Brownian diffusion solute transport. The represented the microbial unit cells in three-dimensional domain modelled after repeating section constant depth film...

ABSTRACT Indirect pathogenicity (IP), the commensal protection of antibiotic-sensitive pathogens by resistant microorganisms low intrinsic virulence, can prevent eradication polymicrobial infections. The contributions antibiotic resistance mechanisms and biofilm structure to IP within biofilms were investigated using a model two-member consortium. Escherichia coli ATCC 33456 was transformed with vectors conferring either ampicillin or spectinomycin resistance, creating two distinct...

Streptococcus mutans and Candida albicans exhibit a symbiotic relationship to form polymicrobial biofilms that exacerbate oral infections including early‐childhood caries, periodontitis candidiasis. Rhamnus prinoides (gesho) has traditionally been used for the treatment of variety illnesses was recently found inhibit Gram‐positive bacterial biofilm formation. We hypothesized extracts have anti‐biofilm activity against S. C. mono‐ dual‐species biofilms. Ethanol were prepared from gesho stems...

We report a dual labeling technique involving two green fluorescent protein (GFP) variants that is compatible with confocal microscopy. Two lasers were used to obtain images of (i) mixed cultures cells, where one species contained GFPuv and another GFPmut2 or GFPmut3, (ii) single containing both in the same cell. This method shows promise for monitoring gene expression as nondestructive situ microscopy multispecies biofilms.

Abstract Escherichia coli has been widely used for heterologous protein production (HPP). To determine whether a biofilm environment could benefit E. HPP using high copy number plasmids, we compared plasmid maintenance and by ATCC 33456 containing pEGFP (a pUC family vector) cultivated in biofilms suspended culture. Cells were grown with or without antibiotic selective pressure flow cells chemostats up to 6 days. In biofilms, increased the (PCN), but 144 h, antibiotic‐free media had...

Bacterial quorum sensing refers to the ability of bacteria control gene expression through detection a threshold concentration certain chemicals called autoinducer(s), which are secreted by self and/or other bacteria. Quorum is implicated in regulation pathologically relevant events such as biofilm formation, virulence, conjugation, sporulation, and swarming mobility. Inhibitors bacterial valuable research tools potential antimicrobial agents. In this paper, we describe discovery several...

ABSTRACT Escapin is an l -amino acid oxidase that acts on lysine to produce hydrogen peroxide (H 2 O ), ammonia, and equilibrium mixtures of several organic acids collectively called escapin intermediate products (EIP). Previous work showed the combination synthetic EIP H functions synergistically as antimicrobial toward diverse planktonic bacteria. We initiated present study investigate how affected bacterial biofilms, using Pseudomonas aeruginosa a model. Specifically, we examined...

Soft X-ray microscopy (SXM) using synchrotron radiation is a newly-available technology for high resolution imaging of hydrated specimens, making it potentially valuable the study biofilms. In this research, SXM was used to investigate bacterial spatial distribution and biofilm structure during early stages colonization an exposed surface. Pseudomonas putida DMP-1 formed closely-packed monolayer on silicon nitride substratum after 24 hours growth. The initial development multilayer,...