A5059886802- Treatment of Major Depression
- Immune cells in cancer
- Mental Health Research Topics
- Atherosclerosis and Cardiovascular Diseases
- Neuroscience and Neuropharmacology Research
- Cell Adhesion Molecules Research
- Receptor Mechanisms and Signaling
- Functional Brain Connectivity Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Single-cell and spatial transcriptomics
- Anxiety, Depression, Psychometrics, Treatment, Cognitive Processes
- Cellular Mechanics and Interactions
- Sphingolipid Metabolism and Signaling
- PARP inhibition in cancer therapy
- Cancer, Lipids, and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Digital Mental Health Interventions
- Ion channel regulation and function
- Caveolin-1 and cellular processes
- Ferroptosis and cancer prognosis
- RNA modifications and cancer
- Schizophrenia research and treatment
- Genomics, phytochemicals, and oxidative stress
- Cholesterol and Lipid Metabolism
- Plant-derived Lignans Synthesis and Bioactivity
University of Toronto
2016-2025
University Health Network
2016-2025
Toronto General Hospital
2017-2025
The University of Texas Southwestern Medical Center
2004-2018
Southwestern Medical Center
2018
Veterans Health Administration
2011
University of Michigan
2000
Background. The present study provides additional data on the psychometric properties of 30-item Inventory Depressive Symptomatology (IDS) and recently developed Quick (QIDS), a brief 16-item symptom severity rating scale that was derived from longer form. Both IDS QIDS are available in matched clinician-rated (IDS-C 30 ; QIDS-C 16 ) self-report (IDS-SR QIDS-SR formats. Method. patient samples included 544 out-patients with major depressive disorder (MDD) 402 bipolar (BD) drawn 19 regionally...
Both thalamic and glutamatergic dysfunction have been implicated in the pathophysiology of schizophrenia. The authors examined ionotropic glutamate receptor expression postmortem samples from patients with schizophrenia comparison subjects, using hypothesis that differs limbic nuclei thalamus schizophrenia.N-Methyl-D-aspartate (NMDA), AMPA, kainate was determined six 12 subjects DSM-III-R diagnoses eight psychiatrically normal individuals. used situ hybridization to determine NMDAR1,...
Abstract Lipid accumulation in macrophages (Mφs) is a hallmark of atherosclerosis, yet how lipid affects inflammatory responses through rewiring Mφ metabolism poorly understood. We modeled cultured wild-type mouse thioglycolate-elicited peritoneal Mφs and bone marrow–derived with conditional (Lyz2-Cre) or complete genetic deficiency Vhl, Hif1a, Nos2, Nfe2l2. Transfection studies employed RAW264.7 cells. were for 24 h oxidized low-density lipoprotein (oxLDL) cholesterol then stimulated LPS....
Abstract The accumulation of lipid and the formation macrophage foam cells is a hallmark atherosclerosis, chronic inflammatory disease. To better understand role in inflammation during atherogenesis, we studied early molecular events that follow oxidized low-density lipoprotein (oxLDL) cultured mouse macrophages. We previously showed oxLDL downregulates response conjunction with downregulation late-phase glycolysis. In this study, show within hours after LPS stimulation, macrophages...
Abstract Lipid accumulation in macrophages (Mφs) is a hallmark of atherosclerosis. Yet, how lipid loading modulates Mφ inflammatory responses remains unclear. We endeavored to gain mechanistic insights into pre-loading with free cholesterol metabolism upon LPS-induced TLR4 signaling. found that activities prolyl hydroxylases (PHDs) and factor inhibiting HIF (FIH) are higher loaded Mφs post-LPS stimulation, resulting impaired HIF-1α stability, transactivation capacity glycolysis. In RAW264.7...
This study evaluated the concordance between self-report and clinician-rated versions of Inventory Depressive Symptomatology (IDS-30) two briefer 16-item Quick (QIDS-16).Data were gathered for 544 adult outpatients with psychotic (N = 106) or nonpsychotic 438) major depressive disorder at 14 public sector mental health clinics in Texas Medication Algorithm Project. Data QIDS-16 extracted from IDS-30. Baseline scores final visit before month 12 analyzed. The each scale compared their...
The formation of macrophage (Mφ) foam cells is a hallmark atherosclerosis, yet how the process lipid loading can modulate Mφ inflammatory responses by rewiring their intracellular metabolic circuits not well understood. Our previous studies have shown that accumulation oxidized LDL (oxLDL) or free cholesterol in Mφs impaired response suppressing HIF-1α-mediated glycolysis and upregulating NRF2 antioxidative response. However, it remains unclear if other processes are also contributory. In...
Leukocyte crawling and transendothelial migration (TEM) are potentiated by shear stress caused blood flow. The mechanism that couples to has not been fully elucidated. We found mice lacking GEF-H1 (GEF-H1-/-), a RhoA-specific guanine nucleotide exchange factor (GEF), displayed limited recruitment of neutrophils into inflamed tissues. GEF-H1-/- leukocytes were deficient in vivo TEM the postcapillary venules. demonstrated although deficiency had little impact on migratory properties under...
OBJECTIVE: While both thalamic abnormalities and dopaminergic dysregulation have been separately implicated in the pathophysiology of schizophrenia, little is known about possible dysfunction molecules associated with neurotransmission thalamus this illness. In study, authors studied question by measuring postmortem brain expression neurotransmission. METHOD: Using situ hybridization receptor autoradiography, determined schizophrenia comparison subjects 1) transcripts encoding five dopamine...
Rationale: Bone marrow transplantation (BMT) is used frequently to study the role of hematopoietic cells in atherosclerosis, but aortic arch lesions are smaller mice after BMT. Objective: To identify earliest stage atherosclerosis inhibited by BMT and elucidate potential mechanisms. Methods Results: Ldlr −/− underwent total body γ-irradiation, bone reconstitution, 6-week recovery. Atherosclerosis was studied ascending compared with without In mice, neutral lipid myeloid cell topography were...
Abstract The Diagnostic and Statistical Manual of Mental Disorders, 5th Edition ( DSM-V ), criterion symptom listings are frequently used in clinical practice as checklists to make diagnoses. However, most conditions are, fact, syndromes, that is, collections signs symptoms commonly occur together the clinic. This report discusses value syndromes medicine psychiatry. It is argued a more precise future enumeration brain circuits pathogenesis psychiatric will help us better understand treat...
Abstract The formation of macrophage (Mφ) foam cells is a hallmark atherosclerosis, yet how the process lipid loading can modulate Mφ inflammatory responses by rewiring their intracellular metabolic circuits not well understood. Our previous studies have shown that accumulation oxidized LDL (oxLDL) or free cholesterol in Mφs impaired response suppressing HIF-1α-mediated glycolysis and upregulating NRF2 antioxidative response. However, it remains unclear if other processes are also...
Myeloid cells (MCs) reside in the aortic intima at regions predisposed to atherosclerosis. Systemic inflammation triggers reverse transendothelial migration (RTM) of intimal MCs into arterial blood, which orchestrates a protective immune response that clears intracellular pathogens from intima. Molecular pathways regulate RTM remain poorly understood. S1P (sphingosine-1-phosphate) is lipid mediator regulates cell trafficking by signaling via 5 G-protein-coupled receptors (S1PRs [S1P...
Use of measurement-based care (MBC) in depression includes the systematic administration validated clinical measurement tools to guide screening, assessment, treatment, and monitoring outcomes patients with depression. In practice, MBC can be used screen for assess severity depressive symptoms, treatment selection change type when improvement is insufficient. addition identifying also help detect co-existing psychiatric conditions inform more precise selection. Furthermore, approach entails...
Hypercholesterolemia (HC) is a key risk factor of atherosclerosis, chronic inflammatory disease that causes myocardial infarction and stroke. Although all regions arteries are exposed to HC, atherosclerosis develops in discrete regions, such as the lesser curvature (LC) mouse aortic arch. Dietary HC Ldlr -/- mice induces intimal myeloid cells develop into foam cells, constituting nascent lesions. The purpose this study characterize transcriptomic changes occurring intima during earliest...
Hypercholesterolemia (HC) is a key risk factor for atherosclerosis, chronic inflammatory disease that causes myocardial infarction and stroke. Although all regions of arteries are exposed to HC, atherosclerosis develops in discrete regions, such as the lesser curvature (LC) mouse aortic arch. In Ldlr -/- mice HC induces intimal myeloid cells develop into foam nascent lesions. The purpose this study characterize transcriptomic changes occurring during earliest stages atherogenesis, correlate...
Populations of CD11c + intimal myeloid cells (MCs) are known to reside in regions the vasculature predisposed atherosclerosis, such as lesser curvature ascending arch. In Ldlr-/- mice, these have been shown take up lipid and contribute formation a nascent lesion under conditions hypercholesterolemia. The role resident CD11c+ normal, healthy aortas has only recently investigated. One function for MCs may be clearance pathogens from subendothelial space via reverse transendothelial migration...