A5060294264- Ion channel regulation and function
- Erythrocyte Function and Pathophysiology
- Catalytic C–H Functionalization Methods
- RNA regulation and disease
- Blood properties and coagulation
- Organoboron and organosilicon chemistry
- RNA Interference and Gene Delivery
- Radical Photochemical Reactions
- CRISPR and Genetic Engineering
- Ion Channels and Receptors
- Virus-based gene therapy research
- Hearing, Cochlea, Tinnitus, Genetics
- Endoplasmic Reticulum Stress and Disease
- Cyclopropane Reaction Mechanisms
- Asymmetric Synthesis and Catalysis
- Chemical Safety and Risk Management
- Cytomegalovirus and herpesvirus research
- Semiconductor materials and devices
- Autophagy in Disease and Therapy
- Calcium signaling and nucleotide metabolism
- GaN-based semiconductor devices and materials
- Neuroscience and Neural Engineering
- Postharvest Quality and Shelf Life Management
- interferon and immune responses
- bioluminescence and chemiluminescence research
Nanjing University of Science and Technology
2025
Broad Institute
2022-2024
Harvard University
2020-2022
Boston Children's Hospital
2020-2022
Purdue University West Lafayette
2019
University of California, San Diego
2018
Duke University
1996-2017
Duke University Hospital
2014-2017
Duke Medical Center
2014-2017
Institute of Neurobiology
2014
Significance Cartilage, a mechanically sensitive tissue that covers joints, is essential for vertebrate locomotion by sustaining skeletal mobility. Transduction of mechanical stimuli cartilage cells, chondrocytes, leads to biochemical–metabolic responses. Such mechanotransduction can be beneficial maintenance when evoked low-level stimuli, or have health-adverse effects via cartilage-damaging high-strain stress. Thus, mechanotrauma relevant the pathogenesis osteoarthritis. Molecular...
Developing vehicles that efficiently deliver genes throughout the human central nervous system (CNS) will broaden range of treatable genetic diseases. We engineered an adeno-associated virus (AAV) capsid, BI-hTFR1, binds transferrin receptor (TfR1), a protein expressed on blood-brain barrier. BI-hTFR1 was actively transported across brain endothelial cells and, relative to AAV9, provided 40 50 times greater reporter expression in CNS
Abstract Piezos are mechanically activated ion channels that function as sensors of touch and pressure in various cell types. However, the precise mechanism structures mediating mechanical activation subsequent inactivation have not yet been identified. Here we use magnetic nanoparticles localized transducers force combination with pressure-clamp electrophysiology to identify sensitive domains important for inactivation.
Piezo proteins form mechanically activated ion channels that are responsible for our sense of light touch, proprioception, and vascular blood flow. Upon activation by mechanical stimuli, rapidly inactivate in a voltage-dependent manner through an unknown mechanism. Inactivation is physiologically important, as it modulates overall sensitivity, gives rise to frequency filtering repetitive itself the target numerous human disease-related channelopathies not well understood mechanistically....
Many presynaptic transcripts have been observed in axons, yet their role synapse development remains unknown. Using visually and pharmacologically isolated terminals from dissociated rat hippocampal neurons, we found that ribosomes β-catenin mRNA preferentially localize to recently formed boutons. Locally translated accumulates at terminals, where it regulates synaptic vesicle release dynamics. Thus, local translation of is a newly described mechanism for axons independently functionalize...
Viral delivery of exogenous coding sequences into the inner ear has potential for therapeutic benefit patients suffering genetic or acquired hearing loss. To devise improved strategies viral delivery, we investigated two injection techniques, round window membrane a novel utricle method, their ability to safely and efficiently transduce sensory hair cells neurons mouse ear. In addition, evaluated three synthetic AAV vectors (Anc80L65, AAV9-PHP.B, AAV2.7m8) encoding enhanced green fluorescent...
AAV-mediated gene therapy is a promising approach for treating genetic hearing loss. Replacement or editing of the Tmc1 gene, encoding hair cell mechanosensory ion channels, effective restoration in mice with some limitations. Efficient rescue outer function and lack recovery later-stage treatment remain issues to be solved. Exogenous genes delivered adeno-associated virus (AAV)9-PHP.B capsid via utricle transduce both inner cells mouse cochlea high efficacy. Here, we demonstrate that...
Human TMEM175, a noncanonical potassium (K + ) channel in endolysosomes, contributes to their pH stability and is implicated the pathogenesis of Parkinson’s disease (PD). Structurally, TMEM175 family exhibits an architecture distinct from canonical channels, as it lacks typical TVGYG selectivity filter. Here, we show that human not only pH-dependent structural changes reduce K permeation at acidic but also displays proton permeation. constitutively conducts 7.4 reduced lower pH. In contrast,...
Abstract Tumor initiating cells (TIC) have been suggested as a mechanism for driving chemoresistance and tumor recurrence in human cancers including triple negative breast cancer (TNBC). Significant progress has made targeting TICs. However, methods simultaneously heterogeneous TIC populations are lacking. In this study, we found that treating TNBC with chemotherapeutic agents led to significant accumulation of the ALDH + population. Treating disulfiram copper mixture (DSF/Cu) specifically...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTEnantioselective Free Radical Allyl Transfers from Allylsilanes Promoted by Chiral Lewis AcidsNed A. Porter, Jason Hongliu Wu, Guiru Zhang, and Aimee D. ReedView Author Information Department of Chemistry, Duke University, Durham, North Carolina 27708Cite this: J. Org. Chem. 1997, 62, 20, 6702–6703Publication Date (Web):October 3, 1997Publication History Received2 July 1997Published online3 October inissue 1...
Arsenic trioxide (ATO) has been demonstrated to induce apoptosis in retinoblastoma cells, however, mechanisms responsible for this phenomenon are not fully understood.In the present study, we determined whether ATO induced by abnormal expression of microRNA.In an model cells subjected 4 μM 72 hours, found 14 miRNAs changed more than 2-fold using miRNA microarray analysis.Most these aberrantly expressed were confirmed quantitative RT-PCR.MiR-376a, a significantly down-regulated miRNA, was...
Developing vehicles that efficiently deliver genes throughout the human central nervous system (CNS) will broaden range of treatable genetic diseases. We engineered an AAV capsid, BI-hTFR1, binds Transferrin Receptor (TfR1), a protein expressed on blood-brain barrier (BBB). BI-hTFR1 was actively transported across brain endothelial cell layer and, relative to AAV9, provided 40-50 times greater reporter expression in CNS
A combined wet and dry cleaning process for GaN(0001) has been investigated with XPS, capacitance voltage measurement. Capacitance–voltage characterization showed that ex-situ sulfide passivation in-situ cyclic trimethylaluminum (TMA)/hydrogen plasma exposure led to reductions in the densities of both interface traps border traps. In situ XPS studies show after sulfur treatment on GaN(0001), desorbs vacuum at 25C prior gate oxide deposition. Ex-situ depth profiling ARXPS post-ALD deposition...
Abstract ChemInform is a weekly Abstracting Service, delivering concise information at glance that was extracted from about 100 leading journals. To access of an article which published elsewhere, please select “Full Text” option. The original trackable via the “References”
Abstract ChemInform is a weekly Abstracting Service, delivering concise information at glance that was extracted from about 100 leading journals. To access of an article which published elsewhere, please select “Full Text” option. The original trackable via the “References”