A5068552479- Plant Pathogens and Fungal Diseases
- Nail Diseases and Treatments
- Fungal Infections and Studies
- Bacteriophages and microbial interactions
- Yeasts and Rust Fungi Studies
- Microbial infections and disease research
- Genomics and Phylogenetic Studies
- Mycorrhizal Fungi and Plant Interactions
- Protein Structure and Dynamics
- Plant Pathogens and Resistance
- Antifungal resistance and susceptibility
- Building materials and conservation
- Heat shock proteins research
- Oral microbiology and periodontitis research
- Viral gastroenteritis research and epidemiology
- Protist diversity and phylogeny
- Toxoplasma gondii Research Studies
- Toxin Mechanisms and Immunotoxins
- Parasitic Infections and Diagnostics
- Oral Health Pathology and Treatment
- Salmonella and Campylobacter epidemiology
- Cerebrospinal fluid and hydrocephalus
- Indoor Air Quality and Microbial Exposure
- Micro and Nano Robotics
Czech Academy of Sciences, Institute of Experimental Medicine
2022-2025
Institute of Experimental Medicine
2022-2024
North-Western State Medical University named after I.I. Mechnikov
2014-2022
Trichophyton mentagrophytes and T. interdigitale are important causative agents of superficial mycoses, demonstrating emergent antifungal drug resistance. We studied the susceptibility profiles in Iranian isolates these two species.A total 96 45 were subjected to molecular typing by ribosomal ITS region. Antifungal for terbinafine, griseofulvin, clotrimazole, efinaconazole, luliconazole, amorolfine ciclopirox obtained CLSI broth microdilution method. The squalene epoxidase (SQLE) gene was...
The fungi Trichophyton mentagrophytes and T interdigitale account for significant amount of dermatophytosis cases worldwide. These two dermatophytes form a species complex have number ribosomal internal transcribed spacer (ITS) region genotypes, allowing simultaneous identification strain typing. Our aim was to describe the geographic distribution mentagrophytes/T ITS genotypes find an association between clinical presentations respective infections. We performed rDNA sequencing in 397...
With new or emerging fungal infections, human and animal pathogens are a growing threat worldwide. Current diagnostic tools slow, non-specific at the species subspecies levels, require specific morphological expertise to accurately identify from pure cultures. DNA barcodes easily amplified, universal, short species-specific sequences, which enable rapid identification by comparison with well-curated reference sequence collection. The primary barcode, ITS region, was introduced in 2012 is now...
Relevance . Obtaining clean lysis spots, with tolerable presence of individual colonies secondary growth, in spot tests is a criterion for assessing the effectiveness therapeutic bacteriophages. At same time, number mechanisms are known by which phage bacteria on lawns occurs without being accompanied virus replication. Aim To assess ratio test results and based detection negative bacteriophage colonies. Conclusion Data 43 bacteriophages extracted from 21 articles were analyzed. Within...
Candida auris is an emergent yeast pathogen, easily transmissible between patients and with high percent of multidrug resistant strains. Here we present a draft genome sequence the first known Russian strain C. auris, isolated from case candidemia. The clustered within South Asian clade seemingly represented independent event dissemination original species range. Observed fluconazole resistance was probably due to F105L K143R mutations in ERG11.
The fungi Trichophyton mentagrophytes and T. interdigitale are closely related species, causing superficial infections in humans other mammals. status of these taxa is a field long-lasting debates. To clarify their phylogenetic relationships within the genus sharpen species boundaries, we performed sequencing four genomes also evaluated three previously published multilocus data sets. We computational delimitation analysis on all available GenBank internal transcribed spacer region (ITS)...
During the past 5 years, an outbreak of recalcitrant dermatophytosis due to a novel Trichophyton species generally resistant terbinafine, T. indotineae, has spread out from South Asia many countries around World. These isolates cannot be reliably differentiated other spp. on basis morphological traits and current laboratory diagnostics relies sequencing ribosomal DNA ITS region.In this study, we aimed introduce two inexpensive rapid PCR-based assays for differentiation between indotineae...
Summary Trichophyton spp. are important causative agents of superficial mycoses. The phylogeny the genus and accurate strain identification, based on ribosomal ITS region sequencing, still under development. present work is aimed at (i) inferring from partial ITS, LSU BT2 sequences (ii) description polymorphism in 15 strains interdigitale . We performed DNA sequence‐based species identification phylogenetic analysis 48 belonging to Phylogenetic relationships were inferred by maximum...
The rapid emergence of antibiotic resistance is major concern globally. Among the most worrying pathogenic bacteria are vancomycin-resistant enterococci. Phage therapy a highly promising method for controlling enterococcal infections. In this study, we described two virulent tailed bacteriophages possessing lytic activity against
Chaperone Hsp70 can cross the plasma membrane of living cells using mechanisms that so far have not received much research attention. Searching part molecule is responsible for transport ability Hsp70, we found a cationic sequence composed 20 amino acid residues on its surface, KST peptide, which was used in further experiments. We showed peptide enters various origins with same efficiency as full-length chaperone. capable carrying cargo molecular weight 30 times greater than own into cells....
Human infections by Trichophyton mentagrophytes occur mainly due to contact with diseased animals. In Iran, T. genotype V is the most prevalent variant of fungus. We aimed determine animal reservoir infection. The study was done on a total 577 dermatophyte strains obtained from animals signs dermatophytosis and human patients. list extensively sampled included sheep, cows, cats dogs. For cases, epidemiological data were collected. All isolates along 70 morphologically similar verrucosum...
Summary Background Dermatophytes are a group of keratinophilic fungi medical importance. Despite relatively long history molecular taxonomic studies, there is still need for information on genetic polymorphism in wider variety genomic loci. Objectives Our goal was to study partial DNA topoisomerase 2 gene ( TOP2 ) dermatophytes. Methods We performed sequencing 26 dermatophyte species along with ribosomal internal transcribed spacer (ITS) sequencing. Results The number polymorphic sites data...
The Trichophyton rubrum species group consists of prevalent causative agents human skin, nail and hair infections, including T sensu stricto violaceum, as well other less well-established or debatable taxa like soudanense, kuryangei megninii. Our previous study provided limited evidence in favour the existence two genetic lineages Russian population.We aimed to structure population identify factors shaping this structure.We analysed polymorphism 12 simple sequence repeat (SSR microsatellite)...
According to the recent changes in classification of family Arthrodermataceae, it may already include 9 genera and 79 species. Dermatophytes associated with human animal infections belong Trichophyton, Microsporum Epidermophyton, whereas Arthroderma, Ctenomyces, Guarromyces, Lophophyton, Nannizzia Paraphyton are mainly represented by geophilic Since morphological physiological traits fungi cannot provide reliable basis for classification, modern system dermatophytes was built on phylogenetic...
One of the central topics in bacteriophage research is host specificity. It depends on success completing viral life cycle stages, including adsorption, penetration genetic material virus into cell and its replication, assembly phage particles lysis. Laboratory assessments spectrum lytic activity phages are inextricably linked to significant methodological biases, often used spot test method can be associated with a large percentage false-positive results. Along variety types specificity,...
Trichophyton rubrum is an important causative agent of superficial mycoses worldwide. To uncover a genetic composition local population this fungus, we sequenced A7C99_6411 and A7C99_6714 loci, coding for hypothetical proteins revealed two complex genotypes, differing by single missense mutation in each locus. One the A7C99_6411/6714 genotypes was not found tinea pedis cases demonstrated mostly longer TRS-1 elements when compared to another genotype. Thus, present description nucleotide...
This study proposes a method for the chamber disinfection of paper with biocide in form fine, volatile droplets using antifungal Rocima GT. provides fungicidal effect, and within short exposure time, fungistatic one. At concentration 5% GT solution, minimum treatment time to ensure complete was 15 min. The proposed by chemical mist less harmful than swab saturated solution. It noted that when at insufficient concentrations inhibit fungal growth completely, can induce, as well suppress,...
This corresponded to the genotypic richness R value 0.91.Minimum combination of 7 loci Tr001, Tr002, Tr003, Tr004, Tr005, Tr006 and TrCT20 was sufficient for characterization all obtained genotypes (Fig. 1).Shannon-Wiener index H′ estimated as 4.038
The fungi from the Trichophyton mentagrophytes / T. interdigitale species complex are prevalent causative agents of skin and hair disease in humans other mammals. Here we review lines evidence supporting recognition three independent within light more recent contradicting data conclude that none them satisfactory. While ITS region genotyping appears to be reasonable when compared identification, knowledge on epidemiological sources, regional distribution antifungal drug resistance genetic...
In the human gut, temperate bacteriophages interact with bacteria through predation and horizontal gene transfer. Relying on taxonomic data, metagenomic studies have associated shifts in phage abundance a number of diseases. The bacteriophage VEsP-1 siphovirus morphology was isolated from sample river water using Enterococcus faecalis as host. Starting whole genome sequence VEsP-1, we retrieved related genomes blastp searches tail protein large terminase sequences, blastn matches compiled...