A5002371087- Advanced MRI Techniques and Applications
- Ion Channels and Receptors
- Pregnancy and preeclampsia studies
- Ion channel regulation and function
- Advanced Fluorescence Microscopy Techniques
- Atomic and Subatomic Physics Research
- MicroRNA in disease regulation
- Neonatal and fetal brain pathology
- Advanced Neuroimaging Techniques and Applications
- Electron Spin Resonance Studies
- Electromagnetic Fields and Biological Effects
- Circular RNAs in diseases
- Neurobiology and Insect Physiology Research
- Magnetic and Electromagnetic Effects
- Pancreatic function and diabetes
- Neonatal Respiratory Health Research
- Cell death mechanisms and regulation
- Receptor Mechanisms and Signaling
- Advanced X-ray Imaging Techniques
- Neuropeptides and Animal Physiology
- Phagocytosis and Immune Regulation
- Nuclear Receptors and Signaling
- S100 Proteins and Annexins
- Chemical Synthesis and Analysis
- Advanced Electron Microscopy Techniques and Applications
University of California, Berkeley
2018-2024
University of California System
2021-2024
Neurosciences Institute
2024
Xiamen University
2012
Children’s Health Research Institute
2007
Western University
1990-2007
Lawson Health Research Institute
1990
University of North Carolina at Chapel Hill
1986-1989
Children's Hospital of Western Ontario
1989
Abstract Although glucagonlike immunoreactants (GLIs) are present in the central nervous system of several mammalian species, their structural relationship with pancreatic proglucagon is not defined, and precise anatomical distribution has been studied extensively. To obtain further information about structure biological significance brain GLIs, three different antigenic determinants proglucagon‐‐glucagonlike peptide I (GLP‐I), glucagon, glicentin‐‐was mapped colchicine‐treated rats by...
Abstract Techniques of in situ hybridization histochemistry, Northern blot hybridization, and immunocytochemistry were used to investigate the biosynthesis glucagonlike immunoreactants (GLIs) rat brain. Cells nucleus tractus solitarius medulla oblongata adult brain hybridized a synthetic oligonucleotide probe (GLP‐I oligomer) corresponding nucleotide sequences pancreatic proglucagon mRNA encoding glucagon‐like peptide I (GLP‐I), stained with antisera specific for two antigenic determinants...
Intraventricular hemorrhage (IVH) is the most common brain injury among premature infants. Neonates with IVH are at greater risk of impaired neurodevelopmental outcomes, compared those without IVH. causes destruction germinal matrix and glial precursor cells, possible effects on cortical development.To investigate development after uncomplicated (with no parenchymal involvement posthemorrhagic hydrocephalus). We hypothesized that would be followed by reduced volume infants near-term age.A...
ABSTRACT Insulin-like growth factors (IGFs) are expressed by, and biologically active on, human fetal cells. The mitogenic actions of IGF-I modulated by the 21–41 kDa class IGF-binding proteins (IGF-BPs). Using a rabbit anti-human IGF-BP antibody raised against highly pure 26 derived from amniotic fluid, we have compared cellular location IGF peptides in tissue sections prostaglandin-induced abortuses 14–16 weeks gestation. monoclonal polyclonal antibodies used were IGF-I, but did not...
Receptor interacting protein 3 (RIP3) is a kinase that plays key role in programmed necrosis. Despite the importance of RIP3-dependent necrosis many pathological processes, current knowledge on function RIP3 very limited. Here we present results proteome-wide analysis RIP3-regulated phosphorylation sites using cells from wildtype (RIP3(+/+)) and knockout (RIP3(-/-)) mice. Because activation requires stimulation by certain extracellular stimuli such as ligands death receptors or Toll-like...
PURPOSE: To retrospectively investigate regional in vivo magnetic resonance (MR) imaging transverse and longitudinal relaxation rates at 3.0 T neonatal brain, the relationship between these rates, their potential use for gray matter (GM) versus white (WM) tissue discrimination. MATERIALS AND METHODS: Informed parental consent performance of procedures was obtained each infant. retrospective image analysis not required; ethics approval from institutional review board. At T, R1 R2 were...
Magnetogenetics was developed to remotely control genetically targeted neurons. A variant of magnetogenetics uses magnetic fields activate transient receptor potential vanilloid (TRPV) channels when coupled with ferritin. Stimulation static or RF neurons expressing these induces Ca
Abstract Tyrosine hydroxylase (TH) is the rate‐limiting enzyme in synthesis of catecholamines neural tissues and adrenal medulla. To study expression TH gene its regulation adult developing tissues, we have synthesized an oligodeoxyribonucleotide probe (oligomer) that specific for mRNA. Using Northern blot hybridization polyadenylated RNAs from gland, brain stem, liver, cerebral cortex with 32 P‐labeled oligomer, a single mRNA 1.9 kb was detected gland stem but not liver cortex. this...
Uterine natural killer (uNK) cells are enriched in the post-ovulatory uterus and during pregnancy. Whether these arise from blood pre-cursors or stem is undefined. To support a hypothesis that precursors of uNK recruited blood, adhesive function CD56+ subsets were assessed one cycle pregnancy.Fifteen women proven fertility provided serial samples menstrual thirty with history implantation failure recurrent spontaneous abortion infertility treatment.CD56(bright) cells, but not CD56(dim) NKT...
Brain radiomics can reflect the characteristics of brain pathophysiology. However, value T1-weighted images, quantitative susceptibility mapping, and R2* mapping in diagnosis Parkinson's disease (PD) was underestimated previous studies. In this prospective study to establish a model for PD based on imaging information, we collected high-resolution data from 171 patients with 179 healthy controls recruited August 2014 2019. According inclusion time, 123 121 were assigned train diagnostic...
Abstract The substantia nigra (SN) is one of the earliest targets 5‐HT neurons raphe nuclei (RN). To test hypothesis that embryonic and catechol‐amine may influence differentiation their target cells or source neurons, we have produced dissociated cell cultures from day 14 (E14) rat rhombencephalon (containing serotonergic RN) mesencephalon dopaminergic nigra, SN). These were grown for 6 days in vitro, either as single (RN SN) cocultures + Effects coculture on morphological development...
To develop a classical geometric interpretation of multiphoton excitation and apply it to MRI. investigate ways in which can enable novel imaging techniques.We present fully view by taking particular rotating frame transformation. In this frame, we find that excitations appear just like single-photon again, therefore, readily generalize concepts already explored standard excitation. With homebuilt low frequency coil, execute slice selective pulse sequence with all its replaced their...
FeRIC (Ferritin iron Redistribution to Ion Channels) is a magnetogenetic technique that uses radiofrequency (RF) alternating magnetic fields activate the transient receptor potential channels, TRPV1 and TRPV4, coupled cellular ferritins. In cells expressing ferritin-tagged TRPV, RF stimulation increases cytosolic Ca2+ levels via biochemical pathway. The interaction between ferritin free that, in turn, trigger chemical reactions producing reactive oxygen species oxidized lipids TRPV. this...
Therapies including hyperventilation (HV) and hypothermia (HT) are currently simultaneously used in brain-injured children at risk for cerebral swelling to reduce blood flow (CBF) alter metabolic rate oxygen (CMRo2). Since HV HT may contribute significant patient morbidity, we evaluated the effects of these treatments combination on CBF, CMRo2, cross-brain extraction (CBo2) using Kety-Schmidt technique before controlled bleeding viscosity 20 lightly anesthetized, paralyzed cats, after...
Abstract Magnetogenetics promises remote control of neurons, but its validity is questioned due to controversies surrounding the underlying mechanisms and deficits in reproducibility. Recent studies discovered that ferritin, used Magnetogenetics, transduces radiofrequency (RF) magnetic fields into biochemical signals (reactive oxygen species oxidized lipids). Magnetic stimulation ferritin-tethered TRPV channels induces Ca 2+ responses modulates behavior electrophysiological indicate a...
Abstract Purpose There are 118 known elements. Nearly all of them have NMR active isotopes and at least 39 different nuclei biological relevance. Despite this, most today's MRI is based on only one nucleus— 1 H. To facilitate imaging potential nuclei, we present a single transmit coil able to excite arbitrary in human‐scale MRI. Theory Methods We completely new type RF coil, the Any‐nucleus Distributed Active Programmable Transmit Coil (ADAPT Coil), with fast switches integrated into...
To develop multiphoton excitation techniques for simultaneous multislice (SMS) imaging and evaluate their performance specific absorption rate (SAR) benefit. improve SMS reconstruction quality with a novel CAIPIRINHA (controlled aliasing in parallel results higher acceleration) design.