A5018703186- Enzyme Structure and Function
- Protein Structure and Dynamics
- DNA Repair Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Mitochondrial Function and Pathology
- Ion channel regulation and function
- Drug Transport and Resistance Mechanisms
- Nanopore and Nanochannel Transport Studies
- Lipid Membrane Structure and Behavior
- Biomarkers in Disease Mechanisms
- Mass Spectrometry Techniques and Applications
- Cellular Mechanics and Interactions
- Alzheimer's disease research and treatments
- DNA and Nucleic Acid Chemistry
- Metabolomics and Mass Spectrometry Studies
- Barrier Structure and Function Studies
- Adenosine and Purinergic Signaling
- RNA and protein synthesis mechanisms
- Carcinogens and Genotoxicity Assessment
- Amyloidosis: Diagnosis, Treatment, Outcomes
- S100 Proteins and Annexins
- Phagocytosis and Immune Regulation
- Extracellular vesicles in disease
Eötvös Loránd University
2015-2021
Pázmány Péter Catholic University
2018
Circular dichroism (CD) spectroscopy is a widely used method to study the protein secondary structure. However, for decades, general opinion was that correct estimation of β-sheet content challenging because large spectral and structural diversity β-sheets. Recently, we showed orientation twisting β-sheets account observed diversity, developed new estimate accurately structure (PNAS, 112, E3095). BeStSel web server provides Beta Structure Selection analyze CD spectra recorded by conventional...
Significance Synaptic pruning is dominant in early ontogenesis when a large number of unnecessary synapses are eliminated, and it maintains synaptic plasticity the mature healthy brain, e.g., memory processes. Its malfunction involved degenerative diseases such as Alzheimer’s disease. C1q, member immune complement system, plays central role selective by microglial phagocytosis. Understanding molecular aspects complement-mediated synapse elimination high importance for developing effective...
Elements of the immune system particularly that innate immunity, play important roles beyond their traditional tasks in host defense, including manifold nervous system. Complement-mediated synaptic pruning is essential developing and healthy functioning brain becomes aberrant neurodegenerative disorders. C1q, component classical complement pathway, plays a central role tagging synapses for elimination; however, underlying molecular mechanisms interaction partners are mostly unknown. Neuronal...
Besides the outstanding potential in biomedical applications, extracellular vesicles (EVs) are also promising candidates to expand our knowledge on interactions between vesicular surface proteins and small-molecules which exert biomembrane-related functions. Here we provide mechanistic details membrane active peptides with antimicrobial effect (MAPs) red blood cell derived EVs (REVs) show that they have capacity remove members of protein corona from REVs even at lower than 5 uM...
Construction of the presynaptic filament (PSF) proper helical structure by Rad51 recombinases is a prerequisite progress homologous recombination repair. We studied contribution ATP-binding to this wt human (hRad51). exploited protein-dissociation effect high hydrostatic pressure determine free energy dissociation protomer interfaces in hRad51 oligomer states and used electron microscopy obtain topological parameters. Without cofactors ATP Ca(2+) template DNA, did not exist monomer form, but...
β2-microglobulin (β2m), the light chain of MHC-I complex, is associated with dialysis-related amyloidosis (DRA). Recently, a hereditary systemic was discovered, caused by naturally occurring D76N β2m variant, which showed structure remarkably similar to wild-type (WT) protein, albeit decreased thermodynamic stability and increased amyloidogenicity. Here, we investigated role mutation in amyloid formation point mutations affecting Asp76-Lys41 ion-pair WT charge cluster on Asp38. Using variety...