A5085009611- Drug-Induced Adverse Reactions
- Mast cells and histamine
- HIV/AIDS Research and Interventions
- Asthma and respiratory diseases
- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- T-cell and B-cell Immunology
- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- Contact Dermatitis and Allergies
- Chemokine receptors and signaling
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Urticaria and Related Conditions
- Law, Economics, and Judicial Systems
- Monoclonal and Polyclonal Antibodies Research
- Antibiotics Pharmacokinetics and Efficacy
- Pharmaceutical studies and practices
- Food Allergy and Anaphylaxis Research
- Polyamine Metabolism and Applications
- Receptor Mechanisms and Signaling
- Pharmacogenetics and Drug Metabolism
- Conflict Management and Negotiation
- Problem and Project Based Learning
- Legal Systems and Judicial Processes
Globe Institute of Technology
2022
Max Planck Unit for the Science of Pathogens
2021
New York State Department of Health
2003-2020
Wadsworth Center
1999-2020
ChemoCentryx (United States)
2012-2015
Dana-Farber Cancer Institute
2011-2014
Harvard University Press
2014
University of California, Berkeley
1984-2013
Brigham and Women's Hospital
2011
Harvard University
2011
Better methods are needed to assess mastcell activation In vivo and distinguish the of mast cells from that basophils. Tryptase, a neutral protease selectively concentrated in secretory granules human (but not basophils), is released by together with histamine serves as marker mast-cell activation. 17 patients systemic mastocytosis, concentrations tryptase plasma were linearly related those (P<0.01). Eleven had levels 4 88 ng per milliliter, indicating ongoing each six who experienced...
Penicillin allergy presents a major obstacle to the successful management of some antepartum infections. We studied 15 pregnant women with histories penicillin confirmed by positive immediate wheal-and-flare skin tests. Thirteen had syphilis, one listeria sepsis, and Streptococcus viridans endocarditis. Each patient was desensitized over four six hours oral administration increasing doses V. At completion procedure, full-dose parenteral therapy G or ampicillin instituted. No extracutaneous...
ABSTRACT How viral and host factors contribute to the severe pathogenicity of H5N1 subtype avian influenza virus infection in humans is poorly understood. We identified three clusters differentially expressed innate immune response genes lungs from (A/Vietnam/1203/04) virus-infected ferrets by oligonucleotide microarray analysis. Interferon were more strongly H5N1-infected ferret than infected with less pathogenic H3N2 subtype. In particular, robust CXCL10 gene expression led us test role...
Abstract Phospholipid metabolism was studied during mediator release from highly purified rat mast cells. The incorporation of 32PO4 into individual phospholipids determined after isolation phospholipid classes by two-dimensional thin layer chromatography. Various stimulators histamine (anti-IgE antibody, concanavalin A [Con A], compound 48/80, or the ionophore A23187) increased phosphatidic acid (PA), phosphatidylinositol (PI), and phosphatidylcholine (PC) 4- to 10-fold within 15 min. No...
Abstract The cyclic adenosine 3′,5′-monophosphate (cAMP) content of isolated unstimulated mast cells and the changes induced by a variety pharmacologic, metabolic, physical stimuli were studied. A modified bovine serum albumin density gradient purification method consistently provided cell preparations which 95% or more pure, without apparent damage, 73% recovery applied to gradients. measured cAMP in was high, mean 16 picomoles per million cells. Moderate agitation contact with glass...
Liver and lung metastases are the predominant cause of colorectal cancer (CRC)-related mortality. Recent research has indicated that CXCR3/chemokines interactions orchestrate haematopoetic cell movement implicated in metastatic process malignant tumours, including CRC cells to lymph nodes. To date, however, contribution CXCR3 liver metastasis not been addressed. determine whether receptors regulate malignancy-related properties cells, we have used CXCR3-expressing lines human (HT29 cells)...
Co-stimulation via the CD28/CTLA-4 system appears critical for T cell proliferation to peptide antigens presented in association with MHC. In this study, we examine roles of CD28 and CTLA-4 response murine cells superantigen staphylococcal enterotoxin B (SEB). vitro, antibodies against B7-1/B7-2 or Fab fragments anti-CD28 significantly inhibit splenocytes SEB. Conversely, anti-CTLA-4 augment proliferative response. Further, addition blocking directed co-stimulated by intact antibodies. These...
Abstract CTLA-4 has been shown to be an important negative regulator of T cell activation. To better understand its inhibitory action, we constructed transgenic mice that display constitutive surface expression on CD4 and CD8 cells. In both in vivo vitro responses, overexpression inhibits This inhibition is dependent B7 CD28, suggesting overexpressed responses by competing with CD28 for binding or interfering signaling. addition, the transgene decreases number CD25+Foxp3+ cells these mice,...
<h3>Background</h3> The yield of nucleic acid amplification testing (NAAT) after routine screening for human immunodeficiency virus (HIV) antibody to detect acute HIV infection (AHI) may vary with different HIV-antibody assays. <h3>Methods</h3> From April 24, 2006, through March 28, 2008, patients underwent using a first-generation assay at 14 county sexually transmitted disease (STD) clinics and 1 community clinic serving homosexual in Los Angeles; second-generation rapid test 3 municipal...
Abstract Metastasis continues to be the leading cause of mortality for patients with cancer. Several years ago, it became clear that chemokines and their receptors could control tumor progress. CXCR3 has now been identified in many cancers including osteosarcoma ligands were expressed by lungs are primary sites which this metastasize. This study tested hypothesis disruption CXCR3/CXCR3 complexes lead a decrease metastasis. The experimental design involved use antagonist, AMG487 2 murine...
The relative timing of genetic alterations that contribute to follicular lymphoma remains unknown. We analyzed a donor-recipient pair who both developed grade 2/3A 7 years after allogeneic transplantation and donor lymphocyte infusions. Both patients harbored identical BCL2/IGH rearrangements also present in 1 2,000 cells the infusion, same V(D)J rearrangement, which underwent somatic hypermutation before clonal divergence. Exome sequencing lymphomas identified 15 shared mutations, 14...
Chemokine (C-C motif) receptor 2 (CCR2) is central for the migration of monocytes into inflamed tissues. The novel CCR2 antagonist CCX140-B, which currently in two separate phase clinical trials diabetic nephropathy, has recently been shown to reduce hemoglobin A1c and fasting blood glucose levels type diabetics. In this report, we describe effects compound on glycemic renal function parameters mice. Since CCX140-B a low affinity mouse CCR2, transgenic human knockin mice were generated...
Negative as well positive co-stimulation appears to play an important role in controlling T cell activation. CTLA-4 has been proposed negatively regulate responses. CTLA-4-deficient mice develop a lymphoproliferative disorder, initiated by the activation and expansion of CD4+ cells. To assess function on CD8+ cells, CTLA-4(-/-) animals were crossed MHC class I-restricted 2C TCR transgenic mouse line. We demonstrate that although primary responses similar, TCR+ cells displayed greater...
A dose-response curve for the hypoprothrombinemic effect of brodifacoum 3-[-3(4'-bromobiphenyl-4-yl) 1,2,3,4-tetrahydronaphth-1-yl] -4-hydroxycoumarin, was constructed using doses ranging from 0.1 to 0.33 mg/kg. Brodifacoum exhibited a remarkably steep curve. failed exhibit dose-dependent on degradation rate constant (kdeg) prothrombin complex activity (PCA) after PCA-synthesis-blocking dose warfarin. Both phenobarbital pretreatment and SKF525A treatment altered anticoagulant response...
In this study ethanol and certain other short-chain aryl (benzyl phenethyl) aliphatic (methyl, propyl, butyl, amyl) alcohols produced up to 10-fold increases in cyclic AMP (cAMP) concentrations purified human peripheral blood lymphocytes. Ethanol as low 80 mg/dl significant elevations lymphocyte cAMP. Significant but less marked augmentation of cAMP response was observed platelets, granulocytes, rabbit alveolar macrophages. The mechanism the alcohol-induced accumulation is probably secondary...