Rationale: Diabetes mellitus is often associated with cardiovascular complications, which the leading cause of morbidity and mortality among patients diabetes mellitus, but little known about mechanism that connects to development dysfunction. Objective: We aim elucidate underlying hyperglycemia-induced cardiac dysfunction on a well-established db/db mouse model for diabetic complications lead heart failure. Methods Results: first profiled expression microRNAs (miRNAs) by microarray...

BACKGROUND: Excessive reactive oxygen species generated in mitochondria has been implicated as a causal event hypertensive cardiomyopathy. Multiple recent studies suggest that microRNAs (miRNAs) are able to translocate modulate mitochondrial activities, but the medical significance of such new miRNA function remained unclear. Here, we characterized spontaneous rats (SHRs) comparison with Wistar rats, finding micro RNA-21 (miR-21) was dramatically induced SHRs relative rats. We designed...

Previous study demonstrated that miR-133a was released into blood from injured myocardium in cardiovascular diseases. However, the dynamic change of circulating level early phase acute myocardial infarction (AMI) and correlation between severity coronary stenosis heart disease (CHD) patients are not clear.Three different cohorts (including 13 AMI patients, 176 angina pectoris 127 control subjects) were enrolled to investigate expression levels with ischemia also relationship plasma stenosis....

Previously, we found that the miR-217 expression level was increased in hearts from chronic heart failure (CHF) patients by using miRNA profile analysis. This study aimed to explore role of cardiac dysfunction. Heart tissue samples CHF were used detect levels. A type 9 recombinant adeno-associated virus (rAAV9) employed manipulate mice with thoracic aortic constriction (TAC)-induced Cardiac structure and function measured echocardiography invasive pressure-volume The levels both TAC mice....

// Huaping Li 1 , Jiahui Fan Zhongwei Yin Feng Wang Chen and Dao Wen Departments of Internal Medicine The Institute Hypertension Tongji Hospital, Medical College, Huazhong University Science Technology, Wuhan, People’s Rep. China Correspondence to: Chen, email: Wang, Keywords : miRNA profile, heart failure, tissue, circulating, Gerotarget Received September 01, 2015 Accepted November 20, Published December 16, Abstract Background: During chronic levels circulating miRNAs endued with...

Cytochrome P450 (CYP) epoxygenases metabolize arachidonic acid to biologically active cis-epoxyeicosatrienoic acids, which have potent vasodilatory, antiinflammatory, antiapoptotic, and antidiabetes properties. Here, we showed the effects of cardiac-specific overexpression CYP epoxygenase 2J2 (CYP2J2) on diabetic cardiomyopathy insulin resistance in high-fat (HF) diet fed, low-dose streptozotocin-treated mice. Diabetic was induced by HF streptozotocin CYP2J2 transgenic Physiological...

Heart failure (HF) is a major public health problem worldwide. The development of HF was related to coronary microvessel dysfunction. Whether miRNAs participate in by regulating function remain unclear.The potential targets miR-665 were predicted rnahybrid software, then verified through anti-Ago2 co-immunoprecipitation, Western blotting and luciferase reporter assays. rAAV9 system used manipulate the expression vivo.Significant increase observed endothelial cells human heart with failure....

Curcumin suppressed ultraviolet (UV) induced skin carcinogenesis and activated the nuclear factor erythroid 2-related 2 (Nrf2) pathway. However, whether curcumin protects injury caused by UV is still unknown. A vitro model was established effects on Hacat cells were detected. Nrf2 knocked down in to verify role protective effect of curcumin. Results indicated that (UVA) (or B (UVB)) irradiation would lead decreased cell proliferation, increased apoptosis, catalase, heme oxygenase 1,...

In the emerging field of RNA drugs, circular (circRNA) has attracted much attention as a novel multifunctional therapeutic target. Delving deeper into intricate interactions between circRNA and disease is critical for driving drug discovery efforts centered around circRNAs. Current computational methods face two significant limitations: lack aggregate information in heterogeneous graph networks higher-order fusion information. To this end, we present approach, metaCDA, which utilizes...

Background Ultraviolet B (UVB) radiation is a major environmental factor contributing to skin damage via DNA damage, oxidative stress, inflammation, and collagen degradation. It penetrates the epidermis, disrupts integrity, generates reactive oxygen species (ROS), activating pro-inflammatory pathways such as NF-κB AP-1, inducing matrix metalloproteinases (MMPs). These processes lead structural changes, pigmentation disorders like melasma. Cumulative from UVB also drives photocarcinogenesis,...

Ultraviolet B (UVB) irradiation is the initial etiological factor for various skin disorders, including erythema, sunburn, photoaging, and photocarcinogenesis. Pterostilbene (Pter) displayed remarkable antioxidant, anti-inflammatory, anticarcinogenic activities. This study aimed to investigate effective mechanism of Pter against UVB-induced photodamage in immortalized human keratinocytes.Human keratinocytes were pretreated with (5 10 μM) 24 h prior UVB (300 mJ/cm2). Harvested cells analyzed...

Long noncoding RNAs (lncRNAs) play crucial roles in cardiovascular diseases. To date, only limited studies have reported the role of mitochondria-derived lncRNAs heart failure (HF). In current study, recombinant adeno-associated virus 9 was used to manipulate lncRNA cytb (lnccytb) expression vivo. Fluorescence situ hybridization (FISH) assay determine location lnccytb, while microRNA (miRNA) sequencing and bioinformatics analyses were applied identify downstream targets. The competitive...

Metabolic reprogramming is a hallmark of cancer characterized by global changes in metabolite levels. However, compared with the study gene expression, profiling metabolites samples remains relatively understudied. We obtained metabolomic and expression data from 454 human solid cell lines across 24 types Cancer Cell Line Encyclopedia (CCLE) database, to evaluate feasibility inferring levels data. For each metabolite, we trained multivariable LASSO regression models identify sets that are...

Infection with human papillomavirus (HPV) is relatively common and certain high-risk HPV strains can induce epithelial dysplasia, increasing the risk of cervical cancer. Green tea polyphenol (GTP) preparations exhibit diverse anti-inflammatory, antioxidative, antitumor properties In Vitro Vivo. Topical GTP application has been recommended as a treatment for genital warts, but effect on infection HPV-associated cancer remains to be established. The present study aimed explore mechanism by...

The A 2A R (adenosine receptor) plays a crucial role in the pathophysiological process of cardiovascular diseases, yet its effect on aortic remodeling remains unclear. We observed elevated adenosine and levels following infusion mice with Ang II (angiotensin II), suggesting potential for adenosine-A system macrophage accumulation subsequent remodeling. effects mechanisms dynamics during were further investigated using knockout by transplantation −/− bone marrow. demonstrated that inhibited...

Gene expression is necessary for regulation in almost all biological processes, at the same time, it related to prognosis head and neck squamous cell carcinoma (HNSCC). The of late-staged HNSCC important because its guiding significance on therapy strategies.In this work, we analyzed relationship between gene Cancer Genome Atlas (TCGA) cohort, optimized panel with random forest survival analysis. Subsequently, a Cox multivariate regression-based model was developed predict clinical outcome...

Background: Excessive reactive oxygen species (ROS) generated in the mitochondria is known to be a causal event hypertensive cardiomyopathy. Multiple recent studies suggest that nuclear genome-encoded miRNAs are able translocate modulate mitochondrial activities, but medical significance of such new miRNA function has remained unclear. Methods: Expression levels representative proteins mitochondrial subunits and ROS production were detected hearts from Wistar rats and spontaneous rats (SHRs)...

Background: Excessive reactive oxygen species (ROS) generated in the mitochondria is known to be a causal event diabetic cardiomyopathy. Multiple recent studies suggest that nuclear genome-encoded miRNAs are able translocate modulate mitochondrial activities(1,2), but medical significance of such new miRNA function has remained unclear. Methods and Results: We observed marked reduction 13 genes heart db/db mice compared with C57 controls. Down-regulation by siRNA recaptured some key disease...