A5052015570- Venomous Animal Envenomation and Studies
- Research on Leishmaniasis Studies
- Healthcare and Venom Research
- Trypanosoma species research and implications
- Bioactive Natural Diterpenoids Research
- Insect and Pesticide Research
- Plant tissue culture and regeneration
- Malaria Research and Control
- Calpain Protease Function and Regulation
- Biochemical and Structural Characterization
- Rabies epidemiology and control
- Toxin Mechanisms and Immunotoxins
- Leptospirosis research and findings
- Mosquito-borne diseases and control
- Insect Pest Control Strategies
- Vector-borne infectious diseases
- Viral Infections and Vectors
- Potato Plant Research
- Forensic Anthropology and Bioarchaeology Studies
- Essential Oils and Antimicrobial Activity
- Climate Change Communication and Perception
- Aquaculture disease management and microbiota
- Phytochemistry Medicinal Plant Applications
- Marine Invertebrate Physiology and Ecology
- Monoclonal and Polyclonal Antibodies Research
Fundação Oswaldo Cruz
2017-2025
Universidad Nacional de Asunción
2010-2021
Centro de Excelência em Bioinformática
2017-2021
Paraguayan Center for Population Studies
2019-2021
Universidade Federal de Rondônia
2017-2020
National University of the Northeast
2020
Instituto Butantan
2020
Centro de Investigación y Desarrollo
2011-2017
Universidad Politécnica de Madrid
2017
Guyra Paraguay
2013
This study aimed to analyze the chemical composition of fixed oils extracted from fruits Acrocomia aculeata, Syagrus cearensis and Attalea speciosa, in addition evaluating their efficacy combating resistant microorganisms, such as Escherichia coli Staphylococcus aureus. The ripe were collected region Barbalha, Ceara , analyzed by gas chromatography mass spectrometry (GC/MS) identify compounds present. antibacterial activity was tested using microdilution method 96-well plates, inhibition...
Chagas disease (CD), caused by Trypanosoma cruzi, affects approximately seven million individuals worldwide, with the highest number of cases in Latin America. CD has two phases, which chronic phase is characterized reduced efficacy drug therapies. This and other factors make developing new strategies that aim to identify molecules capable becoming alternatives or complement current chemotherapy vitally important. Cruzain AsCystatin were obtained recombinantly through expression E. coli....
We report in vivo and vitro antileishmanial trypanocidal activities of a new series N-substituted benzene naphthalenesulfonamides 1-15. Compounds 1-15 were screened against Leishmania infantum , braziliensis guyanensis amazonensis Trypanosoma cruzi . Sulfonamides 6e, 10b, 10d displayed remarkable activity selectivity toward T. epimastigotes amastigotes. 6e showed significant on parasitemia murine model acute Chagas disease. Moreover, 8c, 9c, 12c, 14d interesting IC50 values spp promastigotes...
Phospholipases A2 (PLA2s) are important enzymes present in snake venoms and related to a wide spectrum of pharmacological effects, however the toxic potential therapeutic effects acidic isoforms have not been fully explored understood. Due this, study describes isolation biochemical characterization two new Asp49-PLA2s from Bothrops brazili venom, named Braziliase-I Braziliase-II. The venom was fractionated three chromatographic steps: ion exchange, hydrophobic interaction reversed phase....
Functional and structural diversity of proteins snake venoms is coupled with a wide repertoire pharmacological effects. Snake are targets studies linked to searching molecules biotechnological potential.A homologue phospholipase A2 (BmatTX-IV) was obtained using two chromatographic techniques. Mass spectrometry two-dimensional gel electrophoresis were used determine the molecular mass isoelectric point, respectively. By means Edman degradation chemistry, it possible obtain partial sequence...
Malaria is a parasitic infectious disease and was responsible for 400.000 deaths in 2018. Plasmodium falciparum represents the species that causes most human due to severe malaria. In addition, studies prove resistance of P. drugs used treat malaria, making search new with antiplasmodial potential necessary. this context, literature describes snake venoms as rich source molecules microbicidal potential, including phospholipases A2 (PLA2s). sense, present study aimed isolate basic PLA2s from...
Plasmodium falciparum is known to cause severe malaria, current treatment consists in artemisinin-based combination therapy, but resistance can lead failure. Knowledge concerning P. essential proteins be used for searching new antimalarials, among these a potential candidate shikimate dehydrogenase (SDH), an enzyme part of the pathway which responsible producing endogenous aromatic amino acids. SDH from (PfSDH) unexplored by scientific community, therefore, this study aims establish first...
The complete knowledge of the toxins that make up venoms is base for treatment snake accidents victims and selection specimens preparation venom pools antivenom production. In this work, we used a fast direct venomics approach to identify toxin families in C.d. terrificus venom, Southern American Neotropical rattlesnake. RP-HPLC separation profile pooled from adult followed by mass spectrometry analysis revealed terrificus' proteome composed 12 protein families, which are unevenly...
Several studies have aimed to identify molecules that inhibit the toxic actions of snake venom phospholipases A2 (PLA2s). Studies carried out with PLA2 inhibitors (PLIs) been shown be efficient in this assignment.This work analyze interaction peptides derived from Bothrops atrox PLIγ (atPLIγ) a and evaluate ability these reduce phospholipase myotoxic activities.Peptides were subjected molecular docking homologous Lys49 B. modeled by homology. Phospholipase activity neutralization assay was...
Leishmaniasis is a vector-borne disease caused by parasite protozoon from the genus Leishmania. Among molecular techniques applied for detecting these parasites, real-time PCR with High Resolution Melting (PCR-HRM) proved advantageous since it simultaneously determines both presence and species of pathogen in one step, through amplification later analysis curves generated melting temperature.Based on this technique, goal study was to estimate PCR-HRM sensitivity Leishmania spp. detection...
INTRODUCTION Leishmaniasis, Chagas disease, and malaria cause morbidity globally. The drugs currently used for treatment have limitations. Activity of cinnamic acid analogs against Leishmania spp., Trypanosoma cruzi, Plasmodium falciparum was evaluated in the interest identifying new antiprotozoal compounds. METHODS In vitro effects L. braziliensis, infantum chagasi, T. P. falciparum, hemolytic cytotoxic activities on NCTC 929 were determined. RESULTS Three showed leishmanicidal tripanocidal...
A malária é uma doença endêmica em países subdesenvolvidos causada por parasitos do gênero Plasmodium. Anualmente, milhares de pessoas são infectadas e muitas dessas vão ao óbito decorrência complicações causadas esta patologia. Adicionalmente, as alternativas terapêuticas limitadas, o que ressalta a importância da prospecção novas biomoléculas apresentem eficácia para tratamento desta doença. O objetivo presente trabalho foi isolar purificar duas fospolipases A2 básicas veneno serpente...
Anaplasmataceae bacteria are emerging infectious agents transmitted by ticks. The aim of this study was to identify the molecular diversity bacterial family in ticks and hosts, both domestic wild, as well blood meal sources free-living northeastern Paraguay. were identified using PCR-HRM, a method optimized for purpose, while identification their performed conventional PCR. All amplified products subsequently sequenced. detected hosts included
Abstract The relevance of education and outreach (E&O) activities about the Antarctic Treaty has been recognized at Consultative Meetings (ATCM) Committee for Environmental Protection (CEP). This study examines key topics target audiences detailed in papers submitted to ATCM on E&O. Since entered into force 1961, a total 216 E&O have produced. number increased substantially since mid-1990s. ‘Science’ (76.9%) ‘Wildlife/Biodiversity/Environment’ (75.5%) were most addressed these...