A5100348070- Multiple Myeloma Research and Treatments
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Synthesis and Catalytic Reactions
- Phosphodiesterase function and regulation
- Synthesis of β-Lactam Compounds
- Single-cell and spatial transcriptomics
- Lung Cancer Treatments and Mutations
- Chemokine receptors and signaling
- Lung Cancer Research Studies
- Immune Cell Function and Interaction
- Cancer Genomics and Diagnostics
- Cancer Mechanisms and Therapy
- Colorectal Cancer Treatments and Studies
- Cytokine Signaling Pathways and Interactions
- Ferroptosis and cancer prognosis
- MicroRNA in disease regulation
- CAR-T cell therapy research
- Inflammatory mediators and NSAID effects
- RNA regulation and disease
- Cancer-related molecular mechanisms research
- Signaling Pathways in Disease
- RNA modifications and cancer
- RNA Research and Splicing
- Hearing, Cochlea, Tinnitus, Genetics
New Generation University College
2024-2025
Seoul National University
2024-2025
Seoul National University Bundang Hospital
2024-2025
Catholic University of Korea
2012-2024
Hongik University
2023
Marymount University
2023
Sungkyunkwan University
2009-2022
Samsung Medical Center
2014-2021
Moffitt Cancer Center
2020
Dankook University
2016-2020
Abstract Advanced metastatic cancer poses utmost clinical challenges and may present molecular cellular features distinct from an early-stage cancer. Herein, we single-cell transcriptome profiling of lung adenocarcinoma, the most prevalent histological type diagnosed at stage IV in over 40% all cases. From 208,506 cells populating normal tissues or early to 44 patients, identify a cell subtype deviating differentiation trajectory dominating stage. In stages, stromal immune dynamics reveal...
Abstract Gastric cancer (GC) patients develop malignant ascites as the disease progresses owing to peritoneal metastasis. GC with have a rapidly deteriorating clinical course short survival following onset of ascites. Better optimized treatment strategies for this subset are needed. To define cellular characteristics GC, we used single-cell RNA sequencing characterize tumor cells and tumor-associated macrophages (TAMs) from four samples one sample cerebrospinal fluid. Reference...
Abstract Purpose: In multiple myeloma, extramedullary progression is associated with treatment resistance and a high mortality rate. To understand the molecular mechanisms controlling devastating of we applied single-cell RNA-sequencing (RNA-seq) to myeloma in bone marrow myelomatous pleural effusions or ascites. Experimental Design: Bone samples were collected from 15 patients subjected RNA-seq. The transcriptome data malignant plasma cells surrounding immune microenvironment analyzed....
Valvular inflammation triggered by hyperlipidemia has been considered as an important initial process of aortic valve disease; however, cellular and molecular evidence remains unclear. Here, we assess the relationship between plasma lipids valvular inflammation, identify association low-density lipoprotein with increased lipid macrophage accumulation. Single-cell RNA sequencing analysis reveals heterogeneity leukocytes, interstitial cells, endothelial their phenotypic changes during leading...
Abstract Background Immune checkpoint inhibitors unleash inhibitory signals on T cells conferred by tumors and surrounding stromal cells. Despite the clinical efficacy of inhibitors, lack target expression persistence immunosuppressive limit pervasive effectiveness therapy. These limitations may be overcome alternative approaches that co-stimulate immune microenvironment. Methods We analyzed single-cell RNA sequencing data from multiple human cancers a mouse tumor transplant model to...
Macrophages produce many inflammation-associated molecules, released by matrix metalloproteinases, such as adhesion and cytokines, well, which play a crucial role in atherosclerosis. In this context, we investigated the relationship between Ninjurin-1 (Ninj1 [nerve injury-induced protein]), novel metalloproteinase 9 substrate, expression, atherosclerosis progression.Ninj1 expression progression were assessed atherosclerotic aortic tissue serum samples from patients with coronary artery...
Despite a wealth of knowledge gained in the past three decades concerning molecular underpinnings Alzheimer's disease (AD), progress towards obtaining effective, modifying therapies has proven to be challenging. In this manner, numerous clinical trials targeting production, aggregation, and toxicity betaamyloid, have failed meet efficacy standards. This puts into question beta-amyloid hypothesis suggests that additional treatment strategies should explored. The recent emergence CRISPR/Cas9...
Abstract Endothelial nitric oxide synthase (eNOS) decreases following inflammatory stimulation. As a master regulator of endothelial homeostasis, maintaining optimal eNOS levels is important during cardiovascular events. However, little known regarding the mechanism protection. In this study, we demonstrate regulatory role for expression 2′–5′ oligoadenylate synthetase-like 1 (OASL1) in mRNA stability athero-prone conditions and consider its clinical implications. A lack Oasl1 accelerated...
Ninjury-induced protein 1 (Ninj1) is associated with inflammation and tumor progression shows increased expression in various cancers. This study aimed to investigate the role of Ninj1 colitis-associated colorectal cancer (CRC) by focusing on its interaction 17β-estradiol (E2). Using an azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse model CRC, wild-type (WT) knockout (KO) male mice were treated or without E2. At week 2, KO exhibited attenuated colitis symptoms than WT following...
Cancerstem-like cells (CSLCs) contribute to the initiation and recurrence of tumors their resistance conventional therapies. In this study, small interfering RNA (siRNA)-based screening ~4800 druggable genes in 3-dimensional CSLC cultures comparison 2-dimensional bulk U87 glioma revealed 3 groups essential for following: survival population only, bulk-cultured or both populations. While diverse biologic processes were associated with siRNAs reducing population, CSLC-eliminating enriched a...
Alternative polyadenylation (APA) in 3' untranslated regions (3' UTR) plays an important role regulating transcript abundance, localization, and interaction with microRNAs. Length-variation of 3'UTRs by APA contributes to efficient proliferation cancer cells. In this study, we investigated single cells tumor microenvironment understand the physiological implication different cell types. We analyzed patterns expression level genes from 515 single-cell RNA sequencing (scRNA-seq) dataset 11...
MYO15A variants, except those in the N-terminal domain, have been shown to be associated with congenital or pre-lingual severe-to-profound hearing loss (DFNB3), which ultimately requires cochlear implantation early childhood. Recently, such variants also possibly cause moderate-to-severe loss. Herein, we demonstrate that some mutant alleles can postlingual onset of progressive partial deafness.Two multiplex Korean families (SB246 and SB224), manifesting postlingual, progressive, deafness an...
Somatic mutations of epidermal growth factor receptor (EGFR) occur in ~3% colorectal cancer (CRC) patients. Here, through systematic functional screening 21 recurrent EGFR selected from public data sets, we show that 11 colon cancer-derived mutants (G63R, E114K, R165Q, R222C, S492R, P596L, K708R, E709K, G719S, G724S and L858R) are oncogenic able to transform cells a ligand-independent manner. We demonstrate cellular transformation by these requires dimerization. Importantly, the EGF-induced...
Significance Auditory neuropathy spectrum disorder (ANSD) is a confounding auditory disease in which the subjects respond to sound but have difficulties speech discrimination. Herein, we examined two Asian families with hereditary late-age–onset ANSD. By linkage analysis and exome sequencing, identified TMEM43-p.(Arg372Ter) variant as etiology of disease. To examine mechanism TMEM43 on ANSD, generated knock-in mouse p.(Arg372Ter) that recapitulated progressive hearing loss phenotype...
A systematic understanding of genotype-specific sensitivity or resistance to anticancer agents is required provide improved patient therapy. The availability an expansive panel annotated cancer cell lines enables comparative surveys associations between genotypes and compounds various target classes. Thus, one can better predict the optimal treatment for a specific tumor. Here, we present statistical framework, line enrichment analysis (CLEA), associate response with major genotypes....
Inhibition of the activated epidermal growth factor receptor (EGFR) with either enzymatic kinase inhibitors or anti-EGFR antibodies such as cetuximab, is an effective modality treatment for multiple human cancers. Enzymatic EGFR are lung adenocarcinomas somatic domain mutations while, paradoxically, more in colon and head neck cancers where occur less frequently. In colorectal cancer, routinely used second-line therapy KRAS wild-type tumors. However, detailed mechanisms genomic predictors...
Genetic alterations in lung cancer are distinctly represented non-small cell carcinoma (NSCLC) and small (SCLC). Mutation of the RB1 CDKN2A genes, which tightly associated with cycle regulation, is exclusive to SCLC NSCLC cells, respectively. Through systematic analysis transcriptome proteome datasets for 318 lines, we characterized differential gene expression protein regulation RB1-mutant CDKN2A-mutant NSCLC. Many genes proteins lines belong functional categories transcription, whereas...
The targeting of activated epidermal growth factor receptor (EGFR) with therapeutic anti-EGFR monoclonal antibodies (mAbs) such as cetuximab and panitumumab has been used an effective strategy in the treatment colorectal cancer (CRC). However, its clinical efficacy occurs only a limited number patients. Here, we performed whole-transcriptome analysis xenograft mouse tumors induced by KRASG12D mutation-bearing LS174T CRC cells following either or PBS. Through integrated analyses differential...