A5013841002- Fungal and yeast genetics research
- Polyamine Metabolism and Applications
- Genetics, Aging, and Longevity in Model Organisms
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Cannabis and Cannabinoid Research
- Sirtuins and Resveratrol in Medicine
- Antifungal resistance and susceptibility
- GABA and Rice Research
- Fungal Infections and Studies
- Plant Gene Expression Analysis
- Mitochondrial Function and Pathology
- Plant Stress Responses and Tolerance
- Insect and Arachnid Ecology and Behavior
- Protein Kinase Regulation and GTPase Signaling
- Insect symbiosis and bacterial influences
- Building materials and conservation
- Bacillus and Francisella bacterial research
- Redox biology and oxidative stress
- Cancer Research and Treatments
- Parasitic Diseases Research and Treatment
- Microbial Inactivation Methods
- Effects of Radiation Exposure
- Probiotics and Fermented Foods
- Spaceflight effects on biology
Nawi Graz
2017-2023
University of Graz
2012-2023
Spermidine is a natural polyamine that stimulates cytoprotective macroautophagy/autophagy. External supplementation of spermidine extends lifespan and health span across species, including in yeast, nematodes, flies mice. In humans, levels decline with aging, possible connection between reduced endogenous concentrations age-related deterioration has been suggested. Recent epidemiological data support this notion, showing an increased uptake spermidine-rich food diminishes overall mortality...
The tumor suppressor protein p53 tonically suppresses autophagy when it is present in the cytoplasm. This effect phylogenetically conserved from mammals to nematodes, and human can inhibit yeast, as we show here. Bioinformatic investigations of interactome relationship autophagy-relevant network underscored possible relevance a direct molecular interaction between mammalian ortholog essential yeast Atg17, namely RB1-inducible coiled-coil 1 (RB1CC1), also called FAK family kinase-interacting...
Ageing constitutes the most important risk factor for all major chronic ailments, including malignant, cardiovascular and neurodegenerative diseases. However, behavioural pharmacological interventions with feasible potential to promote health upon ageing remain rare. Here we report identification of flavonoid 4,4'-dimethoxychalcone (DMC) as a natural compound anti-ageing properties. External DMC administration extends lifespan yeast, worms flies, decelerates senescence human cell cultures,...
Natural polyamines (spermidine and spermine) are small, positively charged molecules that ubiquitously found within organisms cells. They exert numerous (intra)cellular functions have been implicated to protect against several age-related diseases. Although polyamine levels decline in a complex age-dependent, tissue-, cell type-specific manner, they maintained healthy nonagenarians centenarians. Increased levels, including through enhanced dietary intake, consistently linked improved health...
Autophagy is a catabolic pathway with multifaceted roles in cellular homeostasis.This process also involved the antiviral response at multiple levels, including direct elimination of intruding viruses (virophagy), presentation viral antigens, fitness immune cells, and inhibition excessive inflammatory reactions.In line its central role immunity, have evolved mechanisms to interfere or evade autophagic process, some cases, even harness autophagy constituents machinery for their...
The naturally occurring polyamine spermidine (Spd) has recently been shown to promote longevity across species in an autophagy-dependent manner. Here, we demonstrate that Spd improves both survival and locomotor activity of the fruit fly Drosophila melanogaster upon exposure superoxide generator neurotoxic agent paraquat. Although a high paraquat concentration (20 mM) was specifically increased female flies only, could be rescued male animals when exposed lower levels (5 mM). These effects...
The lysosomal endoprotease cathepsin D (CatD) is an essential player in general protein turnover and specific peptide processing. CatD-deficiency associated with neurodegenerative diseases, whereas elevated CatD levels correlate tumor malignancy cancer cell survival. Here, we show that the ortholog of budding yeast Saccharomyces cerevisiae (Pep4p) harbors a dual cytoprotective function, composed anti-apoptotic part, conferred by its proteolytic capacity, anti-necrotic which resides protein's...
For this study, we performed a genetic screen of S. cerevisiae's deletion library for mutants sensitive to dehydration stress, with which aimed discover cell dehydration-tolerant genes.We used yeast gene set (YGDS) 4850 viable mutant haploid strains perform genome-wide the identification desiccation stress modifiers. SIP18 is among genes identified as essential cells surviving drying/rehydration process. Deletion promotes accumulation reactive oxygen species and enhances apoptotic necrotic...
The activation of ceramide-generating enzymes, the blockade ceramide degradation, or addition analogues can trigger apoptosis necrosis in human cancer cells. Moreover, endogenous plays a decisive role killing neoplastic cells by conventional anticancer chemotherapeutics. Here, we explored possibility that membrane-permeable C2-ceramide might kill budding yeast (Saccharomyces cerevisiae) under fermentative conditions, where they exhibit rapid proliferation and Warburg-like metabolism is...
Spermidine is a naturally occurring polyamine involved in multiple biological processes, including DNA metabolism, autophagy and aging. Like other polyamines, spermidine also indispensable for successful reproduction at several stages. However, direct influence on the actual fertilization process, i.e., fusion of an oocyte with spermatocyte, remains uncertain. To explore this possibility, we established mating process yeast Saccharomyces cerevisiae as model higher eukaryotes. During human...
Following microbial pathogen invasion, the human immune system of activated phagocytes generates and releases potent oxidant hypochlorous acid (HOCl), which contributes to killing menacing microorganisms. Though tightly controlled, HOCl generation by myeloperoxidase-hydrogen peroxide-chloride neutrophils/monocytes may occur in excess lead tissue damage. It is thus marked importance delineate molecular pathways underlying cytotoxicity both cells. Here, we show that induces reactive oxygen...