A5002136055- Advancements in Transdermal Drug Delivery
- Dermatology and Skin Diseases
- Diabetes Management and Research
- Microfluidic and Capillary Electrophoresis Applications
- Autophagy in Disease and Therapy
- Synthetic Organic Chemistry Methods
- Innovative Microfluidic and Catalytic Techniques Innovation
- Metabolomics and Mass Spectrometry Studies
- Polyamine Metabolism and Applications
- Analytical Chemistry and Chromatography
- Psoriasis: Treatment and Pathogenesis
- Diabetic Foot Ulcer Assessment and Management
- Biosimilars and Bioanalytical Methods
- Pancreatic function and diabetes
- Endoplasmic Reticulum Stress and Disease
- Chemical Synthesis and Analysis
- Metabolism and Genetic Disorders
- Diet and metabolism studies
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Advanced Drug Delivery Systems
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Crystallography and molecular interactions
- Diabetes Treatment and Management
- Spectroscopy Techniques in Biomedical and Chemical Research
Joanneum Research
2015-2024
Medical University of Graz
2015-2024
Institute for Biomedicine
2013-2016
University of Graz
2014
Medical Technologies (Czechia)
2013
NanoTecCenter Weiz Forschungsgesellschaft (Austria)
2013
Sinnar University
2013
In-Q-Tel
2013
Yale University
2010
University of Bergamo
2010
Untargeted metabolomics generates a huge amount of data. Software packages for automated data processing are crucial to successfully process these A variety such software exist, but the outcome strongly depends on algorithm parameter settings. If they not carefully chosen, suboptimal settings can easily lead biased results. Therefore, also require optimization. Several optimization approaches have already been proposed, package which is free intricate experimental labeling steps, fast and...
Healthy aging depends on removal of damaged cellular material that is in part mediated by autophagy. The nutritional status cells affects both and autophagy through as-yet-elusive metabolic circuitries. Here, we show nucleocytosolic acetyl-coenzyme A (AcCoA) production a repressor during yeast. Blocking the mitochondrial route to AcCoA deletion CoA-transferase ACH1 caused cytosolic accumulation precursor acetate. This led hyperactivation AcCoA-synthetase Acs2p, triggering histone...
IL-17A is a key driver of human autoimmune diseases, particularly psoriasis.We sought to determine the role in psoriasis pathogenesis and identify robust measurable biomarker IL-17A-driven pathology.We studied 8 healthy subjects patients with before after administration secukinumab, fully anti-IL-17A mAb, used combination classical techniques novel skin microperfusion assay evaluate expression 170 proteins blood, nonlesional skin, lesional skin. For validation, we also tested stored sera...
Decreased cognitive performance is a hallmark of brain aging, but the underlying mechanisms and potential therapeutic avenues remain poorly understood. Recent studies have revealed health-protective lifespan-extending effects dietary spermidine, natural autophagy-promoting polyamine. Here, we show that spermidine passes blood-brain barrier in mice increases hippocampal eIF5A hypusination mitochondrial function. Spermidine feeding aged affects behavior homecage environment tasks, improves...
OBJECTIVE—To investigate the pharmacodynamic profile and duration of action for five subcutaneous doses insulin detemir (0.1, 0.2, 0.4, 0.8, 1.6 units/kg; 1 unit = 24 nmol) one dose NPH (0.3 IU/kg; IU 6 nmol). RESEARCH DESIGN AND METHODS—This single-center, randomized, double-blind, six-period, crossover study was carried out as a 24-h isoglycemic clamp (7.2 mmol/l) in 12 type diabetic patients. RESULTS—Duration dependent varied from 5.7, to 12.1, 19.9, 22.7, 23.2 h 0.1, units/kg,...
Ageing constitutes the most important risk factor for all major chronic ailments, including malignant, cardiovascular and neurodegenerative diseases. However, behavioural pharmacological interventions with feasible potential to promote health upon ageing remain rare. Here we report identification of flavonoid 4,4'-dimethoxychalcone (DMC) as a natural compound anti-ageing properties. External DMC administration extends lifespan yeast, worms flies, decelerates senescence human cell cultures,...
Functionalized monolithic materials have been prepared by ring-opening metathesis copolymerization of norborn-2-ene (NBE) and 1,4,4a,5,8,8a-hexahydro-1,4,5,8-exo,endo-dimethanonaphthalene (DMN-H6) within borosilicate columns in the presence porogenic solvents such as toluene, methylene chloride, methanol, 2-propanol. Grubbs-type initiators general formula Cl2(PR3)2Ru(CHPh) (R = phenyl, cyclohexyl) were used throughout. The resulting separation media possess microstructures consisting...
A series of norbornene-based, l-valine- and l-phenylalanine-containing chiral monomers have been prepared. Starting from norborn-2-ene-5-carboxylic acid chloride norborn-2-ene-5,6-dicarboxylic anhydride, N-(norborn-2-ene-5-carboxyl)-l-valine (I), N-(norborn-2-ene-5-carboxyl)-l-phenylalanine (II), ethyl ester (III), the N,N-(norborn-2-ene-5,6-dicarbimid) (NBDCI)-protected amino derivatives NBDCI-l-valine (IV), NBDCI-l-phenylalanine (V), NBDCI-l-valine-tert-butylamide (VI),...
The availability of generic topical dermatological drug products is constrained by the limited methods established to assess bioequivalence (BE). A novel cutaneous pharmacokinetic approach, dermal open-flow microperfusion (dOFM), can continuously rate and extent which a becomes available in dermis, compare vivo bioavailability (BA) support BE evaluations for products.To evaluate whether dOFM an accurate, sensitive, reproducible method characterize intradermal BA acyclovir from 5 % creams,...
Polyamines are ubiquitous active biogenic amines which contribute to basic cellular functions. Hence, their quantification in samples of diverse biological origins is essential for understanding how they function, especially disease-relevant conditions. We present here a robust, high-throughput solid-phase extraction online coupled liquid chromatography-tandem mass spectrometry (SPE-LC/MS/MS) approach the simultaneous eight polyamines various samples. The include 1,3-diaminopropane,...
Bariatric surgery is currently one of the most effective treatments for obesity and leads to significant weight reduction, improved cardiovascular risk factors overall survival in treated patients. To date, studies focused on short-term effects bariatric metabolic profile found high variation individual responses surgery. The aim this study was identify relevant changes not only shortly after (Roux-en-Y gastric bypass) but also up year intervention by using untargeted metabolomics. 132 serum...
Ring-opening-metathesis polymerization (ROMP) was used for the preparation of a dipyridylcarbamide-functionalized polymer suitable solid-phase extraction metal ions from aqueous solutions. Resins were prepared by copolymerization functional monomer N,N-di-2-pyridyl-endo-norborn-2-ene-5-carboxamide (I) with 1,4,4a,5,8,8a-hexahydro-1,4,5,8-exo-endo-dimethanonaphthalene (II), using well-defined Schrock catalyst Mo(N-2,6-i-Pr2C6H3)CHCMe2Ph(OCMe(CF3)2)2 (III). The proceeds in living manner,...
Fast and efficient chromatographic separations are made possible with a new class of continuous separation media that synthesized by ring-opening methathesis polymerization (ROMP) which in second step—again through ROMP—undergo tailored functionalization (see scheme).
Immune challenge by bacterial lipopolysaccharide (LPS) causes short-term behavioral changes indicative of depression. The present study sought to explore whether LPS is able induce long-term in depression-related behavior and such an effect depends on mouse strain social context. (0.83 mg/kg) or vehicle was administered intraperitoneally female CD1 C57BL/6 mice that were housed singly groups 4. Depression-like assessed with the forced swim test (FST) 1 28 days post-treatment. Group-housed...
The lysosomal endoprotease cathepsin D (CatD) is an essential player in general protein turnover and specific peptide processing. CatD-deficiency associated with neurodegenerative diseases, whereas elevated CatD levels correlate tumor malignancy cancer cell survival. Here, we show that the ortholog of budding yeast Saccharomyces cerevisiae (Pep4p) harbors a dual cytoprotective function, composed anti-apoptotic part, conferred by its proteolytic capacity, anti-necrotic which resides protein's...
Blood-brain barrier (BBB) impairment in systemic inflammation leads to neuroinflammation. Several factors including cytokines, chemokines and signal transduction molecules are implicated BBB dysfunction response inflammation. Here, we have adopted a novel vivo technique; namely, cerebral open flow microperfusion (cOFM), perform time-dependent cytokine analysis (TNF-alpha, IL-6 IL-10) the frontal cortex of rat brain single peripheral administration lipopolysaccharide (LPS). In parallel,...
Neuronal accumulation of UBB+1, a frameshift variant ubiquitin B, is hallmark Alzheimer's disease (AD). How UBB+1 contributes to neuronal dysfunction remains elusive. Here, we show that in brain regions AD patients with neurofibrillary tangles co-exists VMS1, the mitochondrion-specific component ubiquitin-proteasome system (UPS). Expression yeast disturbs UPS, leading mitochondrial stress and apoptosis. Inhibiting UPS activity exacerbates while stimulating by transcription activator Rpn4...