The tissue or glandular kallikreins (<i>KLK</i>) are members of a highly conserved multigene family encoding serine proteases that central to many biological processes. rodent <i>KLK</i> families large, and clustered at one locus. human gene is on chromosome 19q13.3–13.4, until recently consisted just three members. However, recent studies have identified up 11 new the less than their counterparts. Using Southern blot sequence analysis 10 BACs cosmids spanning approximately 400 kilobases...

Abstract Purpose: Colorectal cancer patients diagnosed with stage I or II disease are not routinely offered adjuvant chemotherapy following resection of the primary tumor. However, up to 10% and 30% relapse within 5 years surgery from recurrent metastatic disease. The aim this study was determine if tumor-associated markers could detect disseminated malignant cells so identify a subgroup early-stage colorectal that were at risk relapse. Experimental Design: We recruited consecutive...

Objective. The human Ureaplasma species are the microbes most frequently isolated from placentae of women who deliver preterm. role has been investigated in pregnancies at <32 weeks gestation, but currently no studies have determined prevalence ureaplasmas moderately preterm and late-preterm (hereafter, "moderate/late preterm") infants, largest cohort infants. Methods. Women delivering moderate/late infants (n = 477) their infants/placentae 535) were recruited, swab specimens chorioamnion...

We have used differential-display PCR (DD-PCR) to compare renal-cell carcinoma (RCC) and normal kidney gene expression with the aim of identifying genes specifically associated RCC. Using a modified DD-PCR approach, which was non-radioactive, quicker simpler than conventional method, 24 cDNA samples were clearly up- or down-regulated in RCC tissue from 4 patients. Fourteen these showed high similarity number known genes. Eight clones chosen for further analysis. These regulator G-protein...

The human tissue kallikrein (<i>KLK</i> ) family of serine proteases, which is important in post-translational processing events, currently consists just three genes—tissue (<i>KLK1)</i> , <i>KLK2</i> and prostate-specific antigen (PSA) (<i>KLK3</i> )—clustered at chromosome 19q13.3–13.4. We identified an expressed sequence tag from endometrial carcinoma cDNA library with 50% identity to the known <i>KLK</i> genes. Primers designed putative exon 2 3 regions this novel kallikrein-related were...

The EphB4 receptor tyrosine kinase is overexpressed in many cancers including prostate cancer. molecular mechanisms by which this ephrin influences cancer progression are complex as there tumor-promoting ligand-independent place well ligand-dependent tumor suppressive pathways.We employed transient knockdown of EPHB4 cells, coupled with gene microarray analysis, to identify genes that were regulated and may represent linked factors. We validated target using qRT-PCR functional assays...

A gene of Colletotrichum gloeosporioides that is induced by nitrogen starvation in axenic culture and expressed at the early stages infection host Stylosanthes guianensis has been identified its role pathogenicity tested. The sequence this gene, named CgDN3, indicated it encodes a protein 74 amino acids contains predicted 18 acid signal for secretion basic 54 mature with weak homology to an internal region plant wall-associated receptor kinases. Mutants C. were produced homologous...

Abstract Overexpression of the receptor tyrosine kinase EphB4 is common in epithelial cancers and linked to tumor progression by promoting angiogenesis, increasing survival facilitating invasion migration. However, other studies have reported loss suggesting a suppressor function some cancers. These opposing roles may be regulated ( i ) presence primary ligand ephrin‐B2 that regulates pathways involved suppression or ii absence allows signaling via ligand‐independent contribute promotion. To...

Abstract Improved therapeutic approaches are needed for the treatment of recurrent and metastatic endometrial cancer. Endometrial cancers display hyperactivation MAPK PI3K pathways, result somatic aberrations in genes such as FGFR2, KRAS, PTEN, PIK3CA, PIK3R1. The FGFR2 have emerged potential targets Activation pathway is seen more than 90% FGFR2mutant cancers. This study aimed to examine efficacy pan-FGFR inhibitor BGJ398 with pan-PI3K inhibitors (GDC-0941, BKM120) p110α-selective BYL719....

Abstract Background Techniques for detecting circulating tumor cells in the peripheral blood of patients with head and neck cancers may identify individuals likely to benefit from early systemic treatment. Methods Reconstruction experiments were used optimise immunomagnetic enrichment RT-PCR detection using four markers ( ELF3 , CK19 EGFR EphB4 ). This method was then tested a pilot study samples 16 advanced carcinomas. Results Seven positive tumour both prior after surgery, 4 but not 3...

Abstract Background The EphB4 receptor tyrosine kinase has been reported as increased in tumours originating from several different tissues and its expression a prostate cancer xenograft model reported. Methods RT-PCR, western blotting immunohistochemical techniques were used to examine protein levels human cell lines LNCaP, DU145 PC3. Immunohistochemistry was also localisation of tissue samples 15 patients with carcinomas. Results All three expressed the gene protein. immunoreactivity vivo...

Abstract Introduction The ability to screen blood of early stage operable breast cancer patients for circulating tumour cells is potential importance identifying at risk developing distant relapse. We present the results a study efficacy immunobead RT-PCR method in with cells. Results Immunomagnetic enrichment followed by (immunobead RT-PCR) panel five epithelial specific markers ( ELF3 , EPHB4 EGFR MGB1 and TACSTD1 ) was used peripheral 56 patients. Twenty were positive two or more markers,...

Background: The multiple banded antigen (MBA), a surface-exposed lipoprotein, is major virulence factor of Ureaplasma spp. We previously demonstrated that the number MBA size variants in amniotic fluid was inversely proportional to severity chorioamnionitis pregnant sheep. However, effect ureaplasma variation on inflammation human pregnancies has not been reported. Methods: Ureaplasmas isolated from chorioamnion women (n=42) were speciated/serotyped and by PCR western blot. Results...

// Sally-Anne Stephenson 1, 2, 3 , Evelyn L. Douglas 4 Inga Mertens-Walker Jessica E. Lisle Mohanan S.N. Maharaj Adrian C. Herington 1 Institute of Health and Biomedical Innovation, Queensland University Technology, Translational Research Institute, Princess Alexandra Hospital, Queensland, Australia 2 Australian Prostate Cancer Centre-Queensland, Faculty Health, Brisbane, The Queen Elizabeth Adelaide, South Australia, Correspondence to: Stephenson, e-mail: s.stephenson@qut.edu.au Keywords:...

Freestanding membranes created from Bombyx mori silk fibroin (BMSF) offer a potential vehicle for corneal cell transplantation since they are transparent and support the growth of human epithelial (HCE) cells. Fibroin derived wild silkworm Antheraea pernyi (APSF) might provide superior material by virtue containing putative cell-attachment sites that absent BMSF. Thus we have investigated feasibility producing transparent, freestanding APSF analysed behaviour HCE cells on this material. No...

Immunomagnetic enrichment followed by RT-PCR (immunobead RT-PCR) is an efficient methodology to identify disseminated carcinoma cells in the blood and bone marrow. The assays must be both specific for tumor sufficiently sensitive enable detection of single cells. We have developed a method test any cancer. This has been investigated using panel markers suitable breast cancer In assay, cell line-derived added 100 peripheral mononuclear (PBMNCs) after which mRNA isolated reverse transcribed...

The FANCA gene is one of the genes in which mutations lead to Fanconi anaemia, a rare autosomal recessive disorder characterised by congenital abnormalities, bone marrow failure, and predisposition malignancy. also potential breast ovarian cancer susceptibility gene. A novel allele was identified has tandem duplication 13 base pair sequence promoter region.We screened germline DNA from 352 patients, 390 patients 256 normal controls determine if presence either these two alleles associated...

Scaffolds prepared from silk fibroin derived cocoons of the domesticated silkworm moth Bombyx mori have demonstrated potential to support attachment and growth human limbal epithelial (HLE) cells in vitro. In this study, we attempted further optimize protocols promote expansion HLE on B. fibroin- (BMSF-) based scaffolds. BMSF films were initially coated with different extracellular matrix proteins then analysed for their impact corneal cell adhesion, morphology, culture confluency. Results...