A5038409288- Preterm Birth and Chorioamnionitis
- Reproductive tract infections research
- Neonatal Respiratory Health Research
- Reproductive System and Pregnancy
- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Infant Nutrition and Health
- Microbial infections and disease research
- Ovarian function and disorders
- Urological Disorders and Treatments
- Pediatric health and respiratory diseases
- Congenital Diaphragmatic Hernia Studies
- Sperm and Testicular Function
- Toxoplasma gondii Research Studies
- Salivary Gland Disorders and Functions
- Neonatal skin health care
- Animal health and immunology
- Wound Healing and Treatments
- Herpesvirus Infections and Treatments
- Streptococcal Infections and Treatments
- Urinary Tract Infections Management
- Plant Pathogens and Fungal Diseases
- Rabies epidemiology and control
- Oral microbiology and periodontitis research
- Pelvic floor disorders treatments
Centers for Disease Control and Prevention
2024
Queensland University of Technology
2011-2020
Wright State University
2015
Christiana Care Health System
2015
Cincinnati Children's Hospital Medical Center
2015
Coombe Women & Infants University Hospital
2015
University of Miami
2015
Royal College of Surgeons in Ireland
2015
Medical University of South Carolina
2015
Duke University
2015
Introduction Xanthine oxidase (XO) is distributed in mammals largely the liver and small intestine, but also highly active milk where it generates hydrogen peroxide (H2O2). Adult human saliva low hypoxanthine xanthine, substrates of XO, high lactoperoxidase substrate thiocyanate, neonates has not been examined. Results Median concentrations xanthine neonatal (27 19 μM respectively) were ten-fold higher than adult (2.1 1.7 μM). Fresh breastmilk contained 27.3±12.2 H2O2 mixing baby with...
Our previous study reported microorganisms in human follicular fluid. The objective of this was to test fluid for the presence and correlate these findings with vitro fertilization (IVF) outcomes. In study, 263 paired fluids vaginal swabs were collected from women undergoing IVF cycles, various causes infertility, cultured detect microorganisms. cause infertility outcomes each woman correlated detected within at time trans-vaginal oocyte retrieval. Microorganisms isolated classified as: (1)...
Objective. The human Ureaplasma species are the microbes most frequently isolated from placentae of women who deliver preterm. role has been investigated in pregnancies at <32 weeks gestation, but currently no studies have determined prevalence ureaplasmas moderately preterm and late-preterm (hereafter, "moderate/late preterm") infants, largest cohort infants. Methods. Women delivering moderate/late infants (n = 477) their infants/placentae 535) were recruited, swab specimens chorioamnion...
Previous studies have measured cytokines expressed within follicular fluid and compared the profiles with aetiology of infertility and/or successful or unsuccessful assisted reproduction technology (ART) outcomes. In this study, 71 paired vaginal secretions collected from ART patients were cultured to detect microorganisms tested for presence cytokines. Patient specimens selected assay based on two criteria: whether specimen was colonized (with prior oocyte retrieval) contaminated by flora...
Abstract In utero and upon delivery, neonates are exposed to a wide array of microorganisms from various sources, including maternal bacteria. Prior studies have proposed that the mode feeding shapes gut microbiota and, subsequently child’s health. However, effect its influence on development neonatal oral in early infancy has not yet been reported. The aim this study was compare healthy infants were exclusively breast-fed or formula-fed using 16S-rRNA gene sequencing. We demonstrated...
Abstract The chorioamnionitis associated with preterm delivery is often polymicrobial ureaplasma being the most common isolate. To evaluate interactions between different proinflammatory mediators, we hypothesized that exposure would increase fetal responsiveness to LPS. Fetal sheep were given intra-amniotic (IA) injections of media (control) or Ureaplasma parvum serovar 3 either 7 70 d before delivery. Another group received an IA injection Escherichia coli LPS 2 prior test for...
Bronchopulmonary dysplasia is associated with chorioamnionitis and fetal lung inflammation. Ureaplasma species are the bacteria most frequently isolated from chorioamnionitis. Very chronic ureaplasma colonization of amniotic fluid causes low-grade inflammation functional maturation in sheep. Less known about shorter exposures lung. Therefore, we hypothesized that ureaplasmas would cause an acute inflammatory response alter development. Singleton ovine fetuses received intra-amniotic parvum...
Human follicular fluid, considered sterile, is aspirated as part of an in vitro fertilization (IVF) cycle. However, it easily contaminated by the trans-vaginal collection route and little information exists its potential to support growth microorganisms. The objectives this study were determine whether human fluid can bacterial over time, steroid hormones estradiol progesterone (present at high levels within fluid) contribute species, species isolated from form biofilms. We found that...
The multiple banded antigen (MBA) is a predicted virulence factor of Ureaplasma species. Antigenic variation the MBA potential mechanism by which ureaplasmas avoid immune recognition and cause chronic infections upper genital tract pregnant women. We tested whether involved in pathogenesis intra-amniotic infection chorioamnionitis injecting virulent or avirulent-derived ureaplasma clones (expressing single variants) into amniotic fluid sheep. At 55 days gestation ewes (n = 20) received...
Ureaplasma species are the bacteria most frequently isolated from human amniotic fluid in asymptomatic pregnancies and placental infections. parvum serovars 3 6 prevalent men women. We hypothesized that effects on fetus chorioamnion of chronic ureaplasma infection dependent serovar, dose, variation multiple-banded antigen (MBA) mba gene. injected high- or low-dose U. serovar 3, 6, vehicle intra-amniotically into pregnant ewes at 55 days gestation (term = 150 days) examined chorioamnion,...
Abstract Neonates are exposed to microbes in utero and at birth, thereby establishing their microbiota (healthy microbial colonisers). Previously, we reported significant differences the neonatal oral of breast-fed formula-fed babies after first discovering a primal metabolic mechanism that occurs when breastmilk (containing enzyme xanthine oxidase) saliva highly elevated concentrations substrates for oxidase: hypoxanthine). The interaction breast milk releases antibacterial compounds...
The consensus of the members International Committee on Systematics Prokaryotes' Subcommittee taxonomy Mollicutes is that recently proposed sweeping changes to nomenclature Mycoplasmatales , specifically involving introduction names Malacoplasma gen. nov., Mesomycoplasma Metamycoplasma Metamycoplasmataceae fam. Mycoplasmoidaceae Mycoplasmoidales ord. Mycoplasmoides Mycoplasmopsis and all species or subspecies comb. nov. placed therein, should be rejected because they violate one more...
Abstract Background Human adenoviruses (HAdVs) can cause outbreaks of flu-like illness in university settings. Most infections healthy young adults are mild; severe illnesses rarely occur. In Fall 2022, an adenovirus outbreak was identified students. Methods HAdV cases were defined as students 17–26 years old who presented to the University Health Service or nearby emergency department with symptoms (eg, fever, cough, headache, myalgia, nausea) and had confirmed by polymerase chain reaction...
Ureaplasma species, the most commonly isolated microorganisms in women with chorioamnionitis, are associated preterm delivery. Chorioamnionitis increases risk and severity of bronchopulmonary dysplasia persistent pulmonary hypertension newborns. It is not known whether timing exposure to inflammation utero an important contributor pathogenesis dysplasia. We hypothesized that chronic would alter air space vascular development after 70 days infection. Pregnant ewes were given intra-amniotic...
Ureaplasma species are the microorganisms most frequently associated with adverse pregnancy outcomes. The multiple banded antigen (MBA), a surface-exposed lipoprotein, is key virulence factor of ureaplasmas. MBA demonstrates size variation, which we have shown previously to be correlated severity chorioamnion inflammation. We aimed investigate U. parvum serovar 3 pathogenesis in vivo, using sheep model, by investigating: variation after long term (chronic) and short (acute) durations utero...
ABSTRACT A PCR assay, using three primer pairs, was developed for the detection of Ureaplasma urealyticum , parvo biovar, mba types 1, 3, and 6, in cultured clinical specimens. The pairs were designed by polymorphic base positions within a 310- to 311-bp fragment 5′ end upstream control region gene. specificity assay confirmed with reference serovars 14 amplified-fragment sizes (81 bp 262 193 6). more sensitive nested also developed. This involved first-step PCR, primers UMS-125 UMA226,...