A5077479342- Immune Cell Function and Interaction
- Phagocytosis and Immune Regulation
- Renal and related cancers
- Genetic and Kidney Cyst Diseases
- Hair Growth and Disorders
- Immunotherapy and Immune Responses
- melanin and skin pigmentation
- Pluripotent Stem Cells Research
- Cleft Lip and Palate Research
- Liver physiology and pathology
- Macrophage Migration Inhibitory Factor
- Craniofacial Disorders and Treatments
- Organ Transplantation Techniques and Outcomes
- Epigenetics and DNA Methylation
- Connective tissue disorders research
- Dermatology and Skin Diseases
- Hedgehog Signaling Pathway Studies
- Skin Protection and Aging
- T-cell and B-cell Immunology
- Gastrointestinal Bleeding Diagnosis and Treatment
- Organ Donation and Transplantation
- Esophageal and GI Pathology
- Renal cell carcinoma treatment
- Hippo pathway signaling and YAP/TAZ
- Wound Healing and Treatments
Rockefeller University
2020-2024
Howard Hughes Medical Institute
2020-2024
Strong Memorial Hospital
2020
University of Rochester
2020
McGill University Health Centre
2016-2017
McGill University
2011-2017
Squamous cell carcinomas are triggered by marked elevation of RAS-MAPK signalling and progression from benign papilloma to invasive malignancy
Lineage plasticity-a state of dual fate expression-is required to release stem cells from their niche constraints and redirect them tissue compartments where they are most needed. In this work, we found that without resolving lineage plasticity, skin cannot effectively generate each in vitro nor regrow hair repair wounded epidermis vivo. A small-molecule screen unearthed retinoic acid as a critical regulator. Combining high-throughput approaches, cell culture, vivo mouse genetics, dissected...
Following tissue damage, epithelial stem cells (SCs) are mobilized to enter the wound, where they confront harsh inflammatory environments that can impede their ability repair injury. Here, we investigated mechanisms protect skin SCs within this environment. Characterization of gene expression profiles hair follicle (HFSCs) migrated into wound site revealed activation an immune-modulatory program, including CD80, major histocompatibility complex class II (MHCII), and CXC motif chemokine...
Through recurrent bouts synchronous with the hair cycle, quiescent melanocyte stem cells (McSCs) become activated to generate proliferative progeny that differentiate into pigment-producing melanocytes. The signaling factors orchestrating these events remain incompletely understood. Here, we use single-cell RNA sequencing comparative gene expression analysis elucidate transcriptional dynamics of McSCs through quiescence, activation, and maturation. Unearthing converging signs increased WNT...
Abstract Billions of cells are eliminated daily from our bodies 1–4 . Although macrophages and dendritic dedicated to migrating engulfing dying debris, many epithelial mesenchymal tissue can digest nearby apoptotic corpses How these non-motile, non-professional phagocytes sense eliminate while maintaining their normal functions is unclear. Here we explore the mechanisms that underlie multifunctionality by exploiting cyclical bouts regeneration degeneration during hair cycling. We show...
Leukocyte antigen related (LAR) family receptor protein tyrosine phosphatases (RPTPs) regulate the fine balance between phosphorylation and dephosphorylation that is crucial for cell signaling during development tissue homeostasis. Here we show LAR RPTPs are required normal of mandibular maxillary regions. Approximately half mouse embryos lacking both Ptprs (RPTPσ) Ptprf exhibit micrognathia (small lower jaw), cleft palate microglossia/glossoptosis deep tongue), a phenotype closely...
Rho family GTPases act as molecular switches regulating actin cytoskeleton dynamics. Attenuation of their signaling capacity is provided by GTPase-activating proteins (GAPs), including p190A, that promote the intrinsic GTPase activity proteins. In current study we have performed a small-scale ENU mutagenesis screen and identified novel loss function allele p190A gene Arhgap35, which introduces Leu1396 to Gln substitution in GAP domain. This results decreased for prototypical Rho-family...
Significance Apoptotic morphogenesis requires strict regulation as both excessive and insufficient cell death is detrimental to tissue function. Here we use the process of ureter maturation, wherein connects bladder by apoptosis-mediated removal intervening common nephric duct (CND), investigate this paradigm. We find that LAR family phosphatases antagonize cIAP1 activity decreasing its stability, thereby releasing brake on CND elimination. In addition, demonstrate Birc2 (cIAP1) mutant...
Summary Paragraph Macrophages and dendritic cells have long been appreciated for their ability to migrate engulf dying debris, including some of the billions that are naturally eliminated from our body daily. However, a substantial number these cleared by ‘non-professional phagocytes’, local epithelial critical organismal fitness. How non-professional phagocytes sense digest nearby apoptotic corpses while still performing normal tissue functions is unclear. Here, we explore molecular...
Abstract Adult stem cells reside in various tissues to govern homeostasis and repair damage. During wound healing, these must be mobilized enter the center of injury where they are exposed many inflammatory immune infiltrating wounded tissue. While indispensable for preventing infections clearing dead cells, can also create a harsh environment which could potentially damage prevent their self-renewal differentiation. Here, using model cutaneous healing hair follicle (HFSCs) wound, we show...