DNA sequences that can form G-quadruplexes (G4s) are highly prevalent in the genome. However, structures and functions of most G4-forming genome poorly understood. Therefore, development molecular probes for G4 recognition biological samples, especially with long wavelength, important basic research G4s. Squaraines dyes exhibit sharp intense absorption strong emission red to NIR region, but very few them have been reported as nucleic acids. Here we report interactions two squaraine dyes, STS...

Lycium barbarum L. is a widely used functional food and medicinal herb in Asian countries. barbarium polysaccharides (LBP) are considered as one of the major components fruit exhibits wide range biological activities. Here, we investigated immunomodulatory effects LBP its uptake behaviors at cellular level. was prepared by water extraction ethanol precipitation, divided into two fractions based on molecular weight distribution ultrafiltration (LBP > 10 kDa < kDa). The physicochemical...

Circadian clocks usually run with a period close to 24 h, but are also plastic and can be entrained by external environmental conditions internal physiological cues. Two key nutrient metabolites, glucose vitamin B3 (nicotinamide), influence the circadian in both mammals plants; however, underlying molecular mechanism is still largely unclear. We reveal that target of rapamycin (TOR) kinase, conserved central growth regulator, essential for glucose- nicotinamide-mediated control Arabidopsis ....

The discovery of heterotrophic nitrification-aerobic denitrification (HN-AD) microorganisms has opened a new window for wastewater treatment. underlying mechanism HN-AD, however, is not fully understood because the phylogenetic diversity HN-AD microbes. isolation and characterization are encouraging furthering understanding this process. In study, we found an Alphaproteobacteria isolate W30 from historically polluted river in China through microbes screening process, which identified as...

Guanine-rich oligonucleotides (GROs) have attracted considerable attention as anticancer agents, because they exhibit cancer-selective antiproliferative activity and can form G-quadruplex structures with higher nuclease resistance cellular uptake. Recently, a GRO, AS1411 has reached phase II clinical trials for acute myeloid leukemia renal cell carcinoma. The of GROs been associated various protein targets; however the real mechanisms action remain unclear. In this study, we showed evidence...

G-quadruplex (G4) forming sequences commonly exist in the genome and are closely related to gene regulation expression. Development of a fluorescent probe that can specifically recognize G4 is essential for studying its structures biological functions. Thiazole orange (TO) an often used nucleic acid dye reported have higher affinity DNA than double-stranded (ds) DNA. Here, four TO derivatives were designed synthesized by introducing different styryl groups obtain highly specific probes. The...

Abstract Serum is a common supplement for cell culture due to it containing the essential active components growth and maintenance of cells. However, knowledges in serum are incomplete. Apart from direct influence on cultured cells, reaction with tested drugs cannot be ignored, which usually results false conclusion activity drugs. Here we report toxicity effect polyamines (spermidine spermine) especially drug-resistant cancer lines, resulted oxidative degradation by amine oxidases...

Abstract Arsenic pollution and its toxicity to aquatic organisms have attracted worldwide attention. The bioavailability of arsenic are highly related speciation. present study investigated the differences in bioaccumulation oxidative stress responses an organism, Daphnia magna , induced by 2 inorganic species (As(III) As(V)). arsenic, Na + /K ‐adenosine triphosphatase (ATPase) activity, reactive oxygen (ROS) content, total superoxide dismutase (SOD) antioxidative capability, malondialdehyde...

A novel fluorescent probe (<bold>L1</bold>) for ratiometric detection and imaging of H₂S in mitochondria was developed by combining a H₂S-sensitive naphthalimide fluorophore targeting cyanine fluorophore.

The G-quadruplex (G4) structures of nucleic acids are considered to play an intrinsic role in gene expression. To this end, the development new G4 ligands has attracted extensive research interests towards potential applications as G4-targeted drugs and molecular probes. date, majority have been composed extended planar aromatic scaffold that interacts with terminal G-tetrad plane via π-π interactions, various side chains interact sugar-phosphate backbone, loops or grooves structures. act...

Zymomonas mobilis is one of the most promising industrial microorganisms for ethanol production. However, biomass, product and substrate oscillation behaviors have been observed under certain operating conditions in both experiments numerical simulations continuous fermentations involving this organism. This article analyzed behavior during fermentation based on Hopf bifurcation analysis, which singularity point limit cycles were introduced. All points parameter values panel simultaneously...

Abstract Background Melatonin has attracted substantial attention because of its excellent prospects for both medical applications and crop improvement. The microbial production melatonin is a safer more promising alternative to chemical synthesis approaches. Researchers have failed produce high yields in common heterologous hosts due either the insolubility or low enzyme activity proteins encoded by gene clusters related biosynthesis. Results Here, combinatorial pathway was successfully...

As endogenous biomolecules, guanine, guanine-based nucleosides, and nucleotides are essential for cellular DNA/RNA synthesis, energy metabolism, signal transduction. However, these biomolecules have been found to a cell-specific antiproliferation effect at higher concentrations, the mechanism is unclear. In this study, we demonstrate that guanine deaminase (GDA) major factor in determining cell-type selectivity of biomolecules. GDA catalyzes deamination xanthine, which an part degradation...

The rice receptor kinase XA21 confers broad-spectrum resistance to Xanthomonas oryzae pv. ( Xoo ), the causal agent of bacterial blight disease. To investigate relationship between expression level and resulting resistance, we generated independent HA-XA21 transgenic lines accumulating immune fused with an HA epitope tag. Whole-genome sequence analysis identified T-DNA insertion sites in sixteen T0 events. Through quantification protein assessment strain PXO99 six lines, observed that...

A series of ER-targeted naphthalimide dyes were developed, and showed promise in tracking ER autophagy under stress.

Noncatalytic carbohydrate-binding modules (CBMs) play important roles in the function of lytic polysaccharide monooxygenases (LPMOs) but have not been well demonstrated for starch-active AA13 LPMO. In this study, four new CBMs were investigated systematically their influence on MtLPMO toward starch terms substrate binding, H2O2 production activity, oxidative product yields, and degradation effect with α-amylase glucoamylase different substrates. Among MtLPMO–CBM chimeras, MtLPMO–CnCBM...

Periodically oscillating concentrations of product, biomass, and substrate have been observed in a continuous fermentations process with Zymomonas mobiliz both experiments simulations. This paper describes the characteristics oscillatory phenomena based on Hopf bifurcation analysis proposes method to regulate dynamic behavior. In this method, these behaviors oscillations were simulated, relationship between amplitude/period operational condition was modeled. model could be used as guide...

Abstract Currently, Faraday isolators and rotators for high‐power lasers are popular research topics; therefore, finding magneto‐optical materials with excellent Verdet constants thermal properties is crucial. In this study, novel holmium scandium aluminum garnet (HoSAG) ceramics were fabricated using vacuum sintering. This study improved the traditional ball milling method pre‐synthesized powders great sinterability after secondary sieving. Different pre‐synthesis temperatures found to have...