A5100349007- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Biosensors and Analytical Detection
- Nanoplatforms for cancer theranostics
- Molecular Sensors and Ion Detection
- Extracellular vesicles in disease
- Nanocluster Synthesis and Applications
- Advanced Nanomaterials in Catalysis
- Sulfur Compounds in Biology
- Synthetic Organic Chemistry Methods
- Job Satisfaction and Organizational Behavior
- MicroRNA in disease regulation
- interferon and immune responses
- Supramolecular Self-Assembly in Materials
- Nutrition, Genetics, and Disease
- RNA modifications and cancer
- Telomeres, Telomerase, and Senescence
- Microbial Natural Products and Biosynthesis
- Carbon and Quantum Dots Applications
- RNA and protein synthesis mechanisms
- Immune cells in cancer
- Microencapsulation and Drying Processes
- Carbohydrate Chemistry and Synthesis
- Analytical Chemistry and Sensors
North Carolina State University
2023-2024
Hunan University
2021-2024
Hainan Medical University
2020-2024
State Key Laboratory of Chemobiosensing and Chemometrics
2022-2024
Shandong Institute of Business and Technology
2022-2024
Hainan General Hospital
2024
Chinese Academy of Sciences
2012-2023
Shanghai Institute of Materia Medica
2017-2023
Research Center for Eco-Environmental Sciences
2012-2023
Shandong University
2022-2023
Mitophagy is a process in which cells remove dysfunctional mitochondria and recycle their constituents lysosome-dependent manner. To probe this process, two different fluorescent dyes specific for lysosomes, respectively, are often used combination. However, current lysosomes cannot distinguish mitochondria-containing autolysosomes from other lysosomes. Therefore, we herein report cyanine dye, HQO, can simultaneously live by exhibiting fluorescence properties. HQO selectively accumulates but...
Specific imaging of cellular senescence emerges as a promising strategy for early diagnosis and treatment various age-related diseases. The currently available probes are routinely designed by targeting single senescence-related marker. However, the inherently high heterogeneity makes them inaccessible to achieve specific accurate detection broad-spectrum senescence. Here, we report design dual-parameter recognition fluorescent probe precise This remains silent in non-senescent cells, yet...
Abstract Anti‐virulence therapy that interferes with bacterial communication, known as “quorum sensing (QS)”, is a promising strategy for circumventing resistance. Using nanomaterials to regulate QS in anti‐virulence has attracted much attention, which mainly attributed unique physicochemical properties and excellent designability of nanomaterials. However, dynamic multistep process, there are significant differences the specific regulatory mechanisms related influencing factors different...
Conventional directed evolution methods offer the ability to select bioreceptors with high binding affinity for a specific target in terms of thermodynamic properties. However, there is lack analogous approaches kinetic selection, which could yield reagents that exhibit slow off-rates and thus remain tightly bound targets extended periods. Here, we describe an vitro methodology uses nuclease flap endonuclease 1 achieve efficient discovery aptamers have dissociation rates. Our...
Aptamers are nucleic acid bioreceptors that have been widely utilized for a variety of biosensing applications, including
Aptamers are nucleic acid-based affinity reagents that have been incorporated into a variety of molecular sensor formats. However, many aptamer sensors exhibit insufficient sensitivity and specificity for real-world applications, although considerable effort has dedicated to improving sensitivity, remained largely neglected understudied. In this work, we developed series using aptamers the small-molecule drugs flunixin, fentanyl, furanyl fentanyl compare their performance─in particular,...
A surface plasmon resonance (SPR) detection system based on a hybridization chain reaction (HCR) was developed for amplified of DNA and small molecules with high sensitivity. This methodology is capable detecting the target in complicated biological samples can be further extended to other proteins or biomarkers.
A new carbon dot (CD)-based nanoprobe for the ratiometric fluorescence detection of DNA was constructed in this work. Thiazole orange (TO), a specific organic small molecular probe toward DNA, is covalently linked to surface CDs, acting as recognition element and response unit. In absence only emitted blue whereas TO almost nonfluorescent. Upon addition turn-on emission at 530 nm appeared gradually enhanced along with increasing target CDs unchanged, which realized DNA. The CD–TO showed good...
The objective of this study is to investigate the feasibility using chitosan–sodium alginate (CS–SA) based matrix tablets for extended-release highly water-soluble drugs by changing formulation variables. Using trimetazidine hydrochloride (TH) as a model drug, influence dissolution medium, amount CS–SA, CS:SA ratio, type SA, and diluents, on in vitro drug release from CS–SA were studied. Drug kinetics mechanisms elucidated. In experiments conducted simulated gastric fluid (SGF) followed...
Neurite outgrowth is the critical step of nervous development. Molecular probes against neurites are essential for evaluation system development, compound neurotoxicity, and drug efficacy on nerve regeneration. To obtain a neurite probe, we developed neurite-SELEX strategy generated DNA aptamer, yly12, that strongly binds neurites. The molecular target yly12 was identified to be neural cell adhesion molecule L1 (L1CAM), surface antigen expressed in normal various cancers. Here, successfully...
A novel, selective and sensitive switch-on fluorescent sensor for Hg2+ switch-off probe biothiols was developed by using [Ru(bpy)2(pip)]2+ as the signal reporter graphene oxide (GO) quencher. Due to affinity of GO towards single-stranded DNA (ss-DNA) [Ru(bpy)2(pip)]2+, three components assembled, resulting in fluorescence quenching. Upon addition Hg2+, a double-stranded (ds-DNA) via T–Hg2+–T base pairs formed intercalated into newly ds-DNA. Then, ds-DNA were removed from surface GO,...
The pH of mitochondria (pHm) is crucial in physiological processes. Here, we report a new mitochondria-targeted ratiometric pH-sensitive fluorescent probe for monitoring the mitochondrial change. This probe, IR-ANNA, was constructed by coupling naphthalimide derivative ANNA and pH-insensitive cyanine dye. IR-ANNA showed good ability, excellent response, low cytotoxicity, which makes suitable pHm living cells. Moreover, has been successfully applied change under stimuli FCCP, NAC, H2O2
Abstract Aortic dissection (AD), a severe cardiovascular disease with the characteristics of high mortality, is lack specific clinical biomarkers. In order to facilitate diagnosis AD, we investigated plasma amino acid profile through metabolomics approach. Total 33 human subjects were enrolled in study: 11 coronary heart (CHD) patients without aortic lesion and acute AD chronic AD. Amino acids identified using liquid chromatography mass spectrometry (LC-MS/MS), further subjected multiple...
Guanine-rich oligonucleotides (GROs) have attracted considerable attention as anticancer agents, because they exhibit cancer-selective antiproliferative activity and can form G-quadruplex structures with higher nuclease resistance cellular uptake. Recently, a GRO, AS1411 has reached phase II clinical trials for acute myeloid leukemia renal cell carcinoma. The of GROs been associated various protein targets; however the real mechanisms action remain unclear. In this study, we showed evidence...
G-quadruplex (G4)-forming sequences are prevalent in the genome and considered to play important roles gene regulation, hence have been viewed as potential therapeutic targets oncology. However, structures functions of most G4s poorly understood. Therefore, development fluorescent probes ligands for is G4 research drug discovery. Herein, we report a new ligand, 2,9-bis[4-(4-methylpiperazin-1-yl)styryl]-1,10-phenanthroline (BMSP), which was synthesized by simple process. BMSP exhibits almost...
Small extracellular vesicles (sEVs) operate as a signaling platform due to their ability carry functional molecular cargos. However, the role of sEVs in hypoxic tumor microenvironment-mediated premetastatic niche formation remains poorly understood.
selection offers a means of discovering functional nucleic acids from randomized libraries, and high-throughput sequencing (HTS) is powerful tool for monitoring the evolution oligonucleotide pools over many cycles enrichment. Many groups now use HTS-derived measures sequence enrichment across different rounds