A5002737277- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Fungal and yeast genetics research
- Peptidase Inhibition and Analysis
- Pneumocystis jirovecii pneumonia detection and treatment
- Advanced Drug Delivery Systems
- Diabetes Management and Research
- Drug Solubulity and Delivery Systems
- Computational Drug Discovery Methods
- Fungal Biology and Applications
- Nanoparticle-Based Drug Delivery
- Bacteriophages and microbial interactions
- Plant-Microbe Interactions and Immunity
- Biofuel production and bioconversion
- Diabetes Treatment and Management
- Bacterial Genetics and Biotechnology
- Toxoplasma gondii Research Studies
- Salmonella and Campylobacter epidemiology
- Photonic and Optical Devices
- Natural Antidiabetic Agents Studies
- Antioxidant Activity and Oxidative Stress
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Dye analysis and toxicity
- Probiotics and Fermented Foods
- Cellular transport and secretion
Centre for DNA Fingerprinting and Diagnostics
2014-2024
Chandigarh University
2016-2023
National Stroke Association of Malaysia
2023
University of Mumbai
2023
Pandit Bhagwat Dayal Sharma Post Graduate Institute of Medical Sciences
2013-2022
All India Institute of Medical Sciences
2022
Guru Nanak Dev University
2021
University of Alabama in Huntsville
2018
Mayo Clinic
2018
WinnMed
2012-2018
Candida glabrata is a yeast pathogen of humans. We have established tissue culture model to analyze the interaction C. with macrophages. Transcript profiling ingested by macrophages reveals global changes in metabolism as well increased expression gene family (YPS genes) encoding extracellular glycosylphosphatidylinositol-linked aspartyl proteases. Eight these YPS genes are found cluster that unique glabrata. Genetic analysis shows required for cell wall integrity, adherence mammalian cells,...
ABSTRACT The pathogenic yeast Candida glabrata exhibits innate resistance to fluconazole, the most commonly used antifungal agent. By screening a library of 9,216 random insertion mutants, we identified set 27 genes which upon mutation, confer altered fluconazole susceptibility in C. . Homologues three these have been implicated azole and/or drug Saccharomyces cerevisiae : two belong family ABC transporters ( PDR5 and PDR16 ), one is involved retrograde signaling from mitochondria nucleus...
Fungal septicemia is an increasingly common complication of immunocompromised patients worldwide. Candida species are the leading cause invasive mycoses with glabrata being second most frequently isolated from Intensive Care Unit patients. Despite its clinical importance, very little known about mechanisms that C. employs to survive antimicrobial and immune response mammalian host. Here, decipher interaction host cells, we have screened a library 18,350 Tn7 insertion mutants for reduced...
Iron is an essential nutrient for all living organisms and human pathogens employ a battery of factors to scavenge iron from the high-affinity iron-binding host proteins. In present study, we have elucidated, via candidate gene approach, major acquisition homoeostatic mechanisms operational in opportunistic fungal pathogen Candida glabrata. Phenotypic, biochemical molecular analysis set 13 C. glabrata strains, deleted proteins potentially implicated metabolism, revealed that reductive uptake...
The QDR (quinidine drug resistance) family of genes encodes transporters belonging to the MFS (major facilitator superfamily) proteins. We show that transporters, which are localized plasma membrane, do not play a role in transport. Hence, null mutants QDR1, QDR2 and QDR3 display no alterations susceptibility azoles, polyenes, echinocandins, polyamines or quinolines, cell wall inhibitors many other stresses. However, deletion genes, individually collectively, led defects biofilm architecture...
The interplay between transcription factors and epigenetic writers like the DNA methyltransferases (DNMTs), role of this in gene expression, is being increasingly appreciated. ZBTB24, a poorly characterized zinc-finger protein, or de novo methyltransferase DNMT3B, when mutated, cause Immunodeficiency, Centromere Instability, Facial anomalies (ICF) syndrome, suggesting an underlying mechanistic link. Chromatin immunoprecipitation coupled with loss-of-function approaches model systems revealed...
ABSTRACT Lissencephaly is a genetically heterogeneous condition caused by aberrant neuronal migration. Cerebellar hypoplasia has been commonly associated in some subtypes of lissencephaly, notably the tubulinopathies. CDK5 microtubule‐associated protein, and its defective function implicated various neurodevelopmental neurodegenerative disorders. Biallelic loss‐of‐function variant reported to cause lissencephaly type 7 single family date. We describe an infant with diffuse agyria, cerebellar...
Mutants of Saccharomyces cerevisiae bearing lesions in the ergosterol biosynthetic pathway exhibit a pleiotropic drug-sensitive phenotype. This has been reported to result from an increased permeability membranes mutant strains different drugs. As disruption yeast multidrug resistance protein, Pdr5p, results similar phenotype, possibility that Pdr5p may be functioning with reduced efficiency these altered sterol backgrounds was examined. To do this, function isogenic S. have disruptions late...
We describe and characterize a method for insertional mutagenesis of the yeast pathogen Candida glabrata using bacterial transposon Tn7. Tn7 was used to mutagenize C. genomic fosmid library. Pools random insertions in individual fosmids were recovered by transformation into Escherichia coli. Subsequently, these introduced recombination genome. found that fragments carrying insertion could integrate genome nonhomologous recombination, single crossover (generating duplication insertionally...
Summary Proteases, key virulence factors of many bacterial and fungal pathogens, are pivotally important for nutrient acquisition, invasion adherence to host cells evasion/escape from immune cells. In this study, we report a novel role CgYps1, member family 11 GPI‐linked aspartyl proteases, in human opportunistic pathogen, Candida glabrata , the regulation pH homeostasis under acidic environmental conditions. We show that CgYps1 is required survive low‐external‐pH environment inability...
Immune evasion is critical for fungal virulence. However, how the human opportunistic pathogen
CgHog1, terminal kinase of the high-osmolarity glycerol signalling pathway, orchestrates cellular response to multiple external stimuli including surplus-environmental iron in human fungal pathogen Candida glabrata ( Cg ). However, CgHog1 substrates remain unidentified. Here, we show that adversely affects adherence host stomach and kidney epithelial cells vitro, but promotes survival iron-rich gastrointestinal tract niche. Further, interactome vitro phosphorylation analysis revealed CgSub2...
Redox pathways play a key role in pathogenesis. Glutathione, central molecule redox homeostasis yeasts, is an essential metabolite, but its requirements can be met either from endogenous biosynthesis or the extracellular milieu. In this report we have examined importance of glutathione two major human opportunistic fungal pathogens, Candida albicans and glabrata. As genome sequence C. glabrata had suggested absence transporters, initially investigated exogenous utilization by disruption...
A family of 11 GPI (glycosylphosphatidylinositol)-linked cell surface-associated aspartyl proteases (yapsins) in the human opportunistic fungal pathogen Candida glabrata is required for wall remodelling, pH homoeostasis, survival macrophages and virulence a murine model disseminated candidiasis. In present paper, we report new roles yapsins C. physiology implicate them first time regulation vacuole homoeostasis. study show that mutant lacking all yapsins, Cgyps1-11∆, possesses an enlarged...
Candida glabrata has emerged as a major fungal pathogen over the last two decades, although our understanding of its survival strategies inside mammalian host remains rudimentary. An important requirement for in vivo is ability to acquire critical nutrients such iron from niches varied content. In present study, we demonstrate first time that C. cells respond high external levels via activation stress-responsive mitogen-activated protein kinases, CgHog1 and CgSlt2, lack either kinase results...