Abstract Background Weaning stress of piglets causes a huge economic loss to the pig industry. Balance and stability intestinal microenvironment is an effective way reduce occurance during weaning process. Clostridium butyricum , as new microecological preparation, resistant high temperature, acid, bile salts some antibiotics. The aim present study investigate effects C. on microbiota their metabolites in weaned piglets. Results There was no statistical significance growth performance...

The effects of different doses a multispecies probiotic (MSP) mixture on growth performance, the incidence diarrhea rate and immune function, fecal microbial diversity structure were evaluated in pre-weaning Holstein dairy calves at WK2, WK4, WK6, WK8. Forty Chinese female newborn randomly assigned to four treatments with 10 each group, C (control group), T1 (0.5 g MSP/calf/day, T2 (1 MSP/calf/day), T3 (2 MSP/calf/day) groups. experimental period was 56 days. Feed intake health scoring...

Weaning stress seriously affects the welfare of calves and causes huge economic losses to cattle breeding industry. Probiotics play an important role in improving animal growth performance, enhancing immune function, gut microbiota. The newly isolated strains Lactobacillus reuteri L81 johnsonii L29 have shown potential as probiotics. Here, we studied probiotic properties these two on weaned calves.Forty were randomly assigned four groups before weaning, with 10 each group, control group...

The objective of this study was to evaluate the effects exogenous non-starch polysaccharidases (a mixture cellulase, xylanase, β-glucanase and mannanase) on growth performance nutrient digestibility, rumen fermentation, microflora sheep. animal trial conducted using 36 5-month-old female fattening hybrid sheep (Duolang♂ × Hu♀) who were randomly assigned into four groups comprising nine per treatment: CON, T1, T2, T3, with 0, 0.1, 0.3, 0.5% NSPases/kg DM TMR, respectively. This complex enzyme...

Cottonseed meal is widely used as an alternative source of protein in the animal feed industry. However, presence toxic gossypol limits its use livestock production. In order to reduce toxicity, microbial degradation generally considered be environmentally friendly and cost-effective strategy. Candida tropicalis ZD-3 has demonstrated ability degrade gossypol. Nevertheless, genome gossypol-induced C. not been fully sequenced, comprehensive metabolic profile remains unexplored. this study,...

The utilization of electroporation for delivering CRISPR/Cas9 system components has enabled efficient gene editing in mammalian zygotes, facilitating the development genome-edited animals. In this study, our research focused on targeting

Butyrate is thought to enhance intestinal mucosal homeostasis, but the detailed mechanism remains unclear. Therefore, further investigation on of butyrate regulation homeostasis was performed.This study used weaned piglets with similar metabolic function humans as a research model. The dietary supplemented 0.2% sodium group (0.2% S) and negative control (CON) were established detect effects growth performance, tissue morphology, barrier function, microbial community structure in...

The effects of supplementing broilers' diets with fermented cottonseed meal (FCSM) on the performance, apparent digestibility, carcass traits, and meat composition were studied. A total 180 yellow-feathered chickens 21 days old randomly divided into three groups (six replicates 10 broilers each): 1) a control group (Con), 2) treatment FCSM by Candida tropicalis (Ct), 3) C. Tropicalis plus Saccharomyces cerevisiae (Ct-Sc). Results showed that supplementation improved average daily gain...

The study investigated the impact of dietary montmorillonite on growth performance, intestinal mucosal barrier, and microbial community in weaned piglets with control group (CON) supplementation 0.2% (0.2% M). Compared CON group, M feed diet increased average daily gain (ADG) days 15–35 day 1–35 intake (ADFI) (0.05 < P 0.1). Besides, higher villus height duodenum jejunum lower crypt depth colon were revealed than ( 0.05). Moreover, V/C (ratio depth) was compared to that both from...

Altered gut microbiota are implicated in inflammatory neonatal calf diarrhea caused by E. coli K99. Beneficial probiotics used to modulate microbiota. However, factors that mediate host-microbe interactions remain unclear. We evaluated the effects of a combination multispecies (MSP) on growth, intestinal epithelial development, immune function and calves infected with Twelve newborn were randomly assigned as follows: C (control, without MSP); D (E. O78:K99 + gentamycin); P supplemental MSP)....

In this study, the immunomodulatory activity of oligopeptide (CP) derived from solid-state fermented cottonseed meal were investigated in immunosuppressed BALB/c mice models by treatment with cyclophosphamide (CY). Results indicated that increased thymus and spleen indices CY-treated mice. The count plague forming cells (PFC) content half serum hemolysis (HC50) immunosuppressive restored to normal level CP-10 CP-20 groups while cytokines interleukin (IL)-2, IL-6, tumor necrosis factor alpha...

Experimental manipulation of the intestinal microbiota influences health host and is a common application for synbiotics. Here Clostridium butyricum (C. butyricum, C.B) combined with corn bran (C.B + Bran) was taken as synbiotics in waned pig model to investigate its regulation over 28 days postweaning. Growth performance, fecal short chain fatty acids (SCFAs) bacterial community were evaluated at day 14 trial. Although C.B Bran treatment has no significant effects on growth performance (P >...

This study aimed to investigate the changes of lipid-related gene and serum metabolites in broiler chickens fed with fermented cottonseed meal (FCSM) diet, through quantitative real-time PCR metabolomics analysis. Totally, 180 1-day-old Cobb broilers were randomly assigned two groups six replicates 15 birds each. The diets consisted a control diet supplemented 0% FCSM (CON group) an experimental 6% (fermented by Candida tropicalis) replacing soybean (FCSM group). results showed that both...

In suckling piglets, transmissible gastroenteritis virus (TGEV) causes lethal diarrhea accompanied by high infection and mortality rates, leading to considerable economic losses. This study explored methods of preventing or inhibiting their production. Bovine antimicrobial peptide-13 (APB-13) has antibacterial, antiviral, immune functions.This analyzed the efficacy APB-13 against TGEV through in vivo vitro experiments.The effects toxicity inhibition rate on swine testicular (ST) cells were...

Succinate has been shown to be a potentially beneficial nutritional supplement with diverse range of physiological functions. However, it remains unknown whether succinate supplementation regulates lipid metabolism in chickens. The aim this study was explore how affects fat deposition and the underlying mechanism involved broilers determine most appropriate level diet. A total 640 one-day-old male yellow-feathered were randomly divided into 4 groups 8 replicates 20 per replicate. basal diet...

Weaning is one of the most stressful periods in yak growth. However, impact weaning on microbial diversity, structure, and potential function feces not clear. In this study, 12 Xinjiang yaks aged 3, 4, 5, 6 months old were selected to collect fresh before after weaning. Through 16S rRNA ITS high-throughput sequencing, dynamic distribution fecal, bacterial, fungal communities each month revealed. The study found that richness fungi had a significant At phylum level, Firmicutes, Bacteroidetes,...

The fermentation of grape seed meal, a non-conventional feed resource, improves its conventional nutritional composition, promotes the growth and development livestock fat metabolism by influencing structure diversity intestinal bacteria. In this study, components Fermented meal (FGSM) their effects on performance, carcass quality, serum biochemistry, bacteria yellow feather broilers were investigated. A total 240 male 14-day-old yellow-feathered randomly selected divided into four groups,...

Abstract BACKGROUND Probiotics exhibit antibiotic properties and are capable of treating certain bacterial infections, including diarrhea. Therefore, the aim this study is to investigate effects dietary supplementation with multispecies probiotic (MSP) on diarrhea, average daily gain (ADG) intestinal development neonatal calves challenged Escherichia coli K99. RESULTS Thirty‐six Holstein were randomly assigned three treatment groups. After E. K99 challenge, in control (C) MSP groups had...

Calf intestines are colonized by rich and complex microbial communities, playing a crucial role in animal physiology, metabolism, nutrition, immune function. In this study, we provide insight into the composition of fecal bacteria fungi, respectively, as well cross-kingdom interactions. We investigated intestinal microbiota different breeds calves characterizing bacterial fungal communities rectal feces Holstein German Simmental × cross F1 generation (GXH) using 16S rRNA ITS amplicon...

Abstract This study investigated the effects of cottonseed meal protein hydrolysate (CPH) on growth performance, carcass characteristics, serum biochemical indices, intestinal morphology, and enzyme activities yellow‐feather broilers. We randomly divided 240 chicks into four groups, each with six replicates: a basal diet 0% (CON), 1% (LCPH), 3% (MCPH), or 5% (HCPH) CPH. The trail spanned 63 days included three phases: Days 1–21, 22–42, 43–63. Increased average daily gain (ADG) decreased...