Foot-and-mouth disease is a frequently occurring of cloven-hoofed animals that caused by infection with the foot-and-mouth virus (FMDV). FMDV circumvents type-I IFN response expressing proteins antagonize cellular innate immunity, such as leader protease and 3C protease. We identified structural protein VP3 negative regulator virus-triggered IFN-β signaling pathway. Expression inhibited Sendai activation regulatory factor-3 expression retinoic acid-inducible gene-I/melanoma...

Channel catfish (Ictalurus punctatus) is a widely consumed freshwater fish known for its nutritional value but highly prone to spoilage. This study investigated the quality changes of muscle tissue under refrigeration and freezing through physicochemical, metabolomic, microbial analyses. Results revealed that sensory scores decreased significantly during storage, with frozen samples maintaining similar refrigerated ones after extended periods. Protein degradation lipid oxidation, indicated...

Abstract Background The widespread use of antibiotics has led to the emergence many drug-resistant strains; thus, development new antibacterial drugs is essential with antimicrobial peptides becoming focus research. This study assessed effect a novel peptide, named LL-1 on Escherichia coli ( E.coli ) by determining minimum inhibitory concentration (MIC) and curve. interaction between E. DNA was then detected nucleic acid gel electrophoresis. cell membrane detecting leakage β-galactosidase,...

In suckling piglets, transmissible gastroenteritis virus (TGEV) causes lethal diarrhea accompanied by high infection and mortality rates, leading to considerable economic losses. This study explored methods of preventing or inhibiting their production. Bovine antimicrobial peptide-13 (APB-13) has antibacterial, antiviral, immune functions.This analyzed the efficacy APB-13 against TGEV through in vivo vitro experiments.The effects toxicity inhibition rate on swine testicular (ST) cells were...

The circular replication-associated protein (Rep)-encoding single-stranded (CRESS) DNA virus emergence in diverse host has been associated with severe disease. Porcine circovirus-like (Po-Circo-like [PCL] virus) is a CRESS virus, the prevalence and pathogenicity of which are rarely studied.We obtained two blood samples, four faecal intestinal samples from pig farm suffered diarrheal disease delivery room September 2020 attempted to isolate identify causative pathogen. Subsequently, only PCL...

Abstract Background Porcine epidemic diarrhea virus (PEDV) is one of the most important enteric viruses causing in pigs. The establishment a rapid detection method applicable field conditions will be conducive to early pathogen and implementation relevant treatment. A novel nucleic acid amplification method, recombinase polymerase (RPA), has been widely used for infectious disease diagnosis. Results In present study, reverse transcription (RT)-RPA assay combined with lateral flow dipstrip...

The causative agent of Glasser's disease in swine is Haemophilus parasuis. Commercial bacterins are widely used for protection the population. However, cross limited because H. parasuis has more than 15 serovars. Transferrin-binding protein A shown potential as a broad-spectrum vaccine candidate against homologous and heterologous strains. Here we amplified full-length tbpA gene from an serovar 13 isolate cloned it into pET-SUMO expression vector. We then expressed purified TbpA by Ni...

Abstract Background The recent emergence of antibiotic-resistant strains bacteria has increased the need to develop effective alternatives antibiotics. Antimicrobial peptides have been considered as a promising product with several advantages. Results In this present study, we identified novel cecropin from armyworm, Mythimna separata (armyworm 1, AC-1) by transcriptome sequencing and multi-sequence alignment analysis. AC-1 precursor comprised 63 amino acid residues, containing conserved...

Porcine transmissible gastroenteritis virus (TGEV) is an enteric coronavirus that has caused high morbidity and mortality of piglets worldwide. Previous studies have shown the TGEV can lead to severe diarrhoea, vomiting dehydration in 2-week-old weaned piglets, resulting a large number piglet deaths. Antimicrobial peptides broad-spectrum antimicrobial activity strong killing effect on bacteria, especially drug-resistant pathogenic it attracted broad concern. However, there are very few...

Integrin 3 plays a major role in various signaling pathways, cell apoptosis, and tumor angiogenesis.To examine the functions roles of integrin, stable CHO-677 line expressing murine heterodimer (designated as "CHO-677-m3" cells) was established using highly efficient lentiviral-mediated gene transfer technique.Integrin subunits  3 were detected at protein levels by polymerase chain reaction (PCR) indirect immunofluorescent assay (IFA), respectively, CHO-677-m3 20th passage,...

The strategy of blocking myostatin (MSTN) signal transduction has long been regarded as a promising approach in the treatment patients with muscle loss. However, individuals taking agents often encounter issues such lack strength, fatigue, and poor proliferation due to hypertrophy involvement multiple receptors. To address these challenges, series experiments were conducted on C2C12 cell line this study. First, pX601-SaCas9-sgRNA/puro vector carrying Cas9-encoded gene was constructed...

Monogenean infection can cause high mortality and significant financial losses in commercially farmed fish.Existing chemical drugs for the treatment of such infections often have serious drawbacks.In order to find alternative agents drugs, fourteen medicinal plants were tested their vivo anthelmintic activity against Gyrodactylus kobayashii goldfish (Carassius auratus).Ethanol extracts Evodia rutaecarpa, Cnidium monnieri Sophora flavescens had 100% efficacy at low concentrations (100, 100...

Integrin αυβ8 may play an important role in the initial stage of FMDV infection and influence host specificity tissue tropism.To study integrin infection, a cell line, which stably expressed suckling mouse αvβ8 heterodimer, was established designated as CHO-677-mαυβ8.Polymerase chain reaction (PCR) indirect immunofluorescent assay (IFA) were used to determine presence subunits αv β8 line at 20 th passage, respectively.The results showed that these genes successfully transduced...

Abstract Background: The recent emergence of antibiotic-resistant strains bacteria has increased the need to develop effective alternatives antibiotics. Antimicrobial peptides have been considered as a promising product with several advantages.Results: In this present study, we identified novel cecropin from armyworm, Mythimna separata (armyworm 1, AC-1) by transcriptome sequencing and multi-sequence alignment analysis. AC-1 precursor comprised 63 amino acid residues, containing conserved...

The diarrhoea of pigs, in particular, for the newborns, is very harmful to pig industry. Porcine circovirus-like virus (Po-Circo-like (PCL) virus) a circular replication-associated protein (Rep)-encoding single-stranded (CRESS) DNA virus. Two PCL strains, with severe and hemorrhagic enteritis, have been found two different farms Guangdong province, China. Subsequently, full genomes strains (PCL GD06 GD09) were sequenced. viruses contains 3942 nucleotides 3925 nucleotides, which vary from...

Abstract Background: The recent emergence of antibiotic-resistant strains bacteria has increased the need to develop effective alternatives antibiotics. Antimicrobial peptides have been considered as a promising product with several advantages. Results: In this present study, we identified novel cecropin from armyworm, Mythimna separata (armyworm 1, AC-1) by transcriptome sequencing and multi-sequence alignment analysis. AC-1 precursor comprised 63 amino acid residues, containing conserved...