A5101582542- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- COVID-19 Clinical Research Studies
- Legionella and Acanthamoeba research
- Animal Virus Infections Studies
- Liver Disease Diagnosis and Treatment
- Plant tissue culture and regeneration
- Bacterial biofilms and quorum sensing
- Pneumonia and Respiratory Infections
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Advanced Proteomics Techniques and Applications
- Genomics and Phylogenetic Studies
- Bacterial Infections and Vaccines
- Hepatocellular Carcinoma Treatment and Prognosis
- Glycosylation and Glycoproteins Research
- Acute Kidney Injury Research
- Chronic Kidney Disease and Diabetes
- Dialysis and Renal Disease Management
- Brain Metastases and Treatment
- Genetic Neurodegenerative Diseases
- Metabolomics and Mass Spectrometry Studies
- Urinary Tract Infections Management
- Transgenic Plants and Applications
National Center for Advancing Translational Sciences
2022-2025
Stanford University
2024
National Institutes of Health
2015-2024
Hefei National Center for Physical Sciences at Nanoscale
2024
University of Science and Technology of China
2024
Palo Alto University
2024
Fujian Provincial Hospital
2023
University of Hong Kong
2020-2022
HKU-Pasteur Research Pole
2020-2022
J. Craig Venter Institute
2019-2022
N-acylhomoserine lactones (AHLs) are used as signal molecules by many quorum-sensing Proteobacteria. Diverse plant and animal pathogens use AHLs to regulate infection virulence functions. These signals subject biological inactivation AHL-lactonases AHL-acylases. Previously, little was known about the molecular details underlying latter mechanism. An AHL signal-inactivating bacterium, identified a Ralstonia sp., isolated from mixed-species biofilm. The encoding gene this organism, which we...
The World Health Organization has declared the ongoing outbreak of COVID-19, which is caused by a novel coronavirus SARS-CoV-2, pandemic. There currently lack knowledge about antibody response elicited from SARS-CoV-2 infection. One major immunological question concerns antigenic differences between and SARS-CoV. We address this analyzing plasma patients infected or SARS-CoV immunized mice. Our results show that, although cross-reactivity in binding to spike protein common,...
Abstract The World Health Organization has recently declared the ongoing outbreak of COVID-19, which is caused by a novel coronavirus SARS-CoV-2, as pandemic. There currently lack knowledge in antibody response elicited from SARS-CoV-2 infection. One major immunological question concerning antigenic differences between and SARS-CoV. We address this using plasma patients infected or SARS-CoV, obtained immunized mice. Our results show that while cross-reactivity binding to spike protein...
Abstract SIGNAL is a multicenter, randomized, double-blind, placebo-controlled phase 2 study (no. NCT02481674) established to evaluate pepinemab, semaphorin 4D (SEMA4D)-blocking antibody, for treatment of Huntington’s disease (HD). The trial enrolled total 265 HD gene expansion carriers with either early manifest (EM, n = 179) or late prodromal (LP, 86) HD, randomized (1:1) receive 18 monthly infusions pepinemab ( 91 EM, 41 LP) placebo 88 45 LP). Pepinemab was generally well tolerated,...
The human microbiome has been the focus of numerous research efforts to elucidate pathogenesis diseases including cancer. Oral cancer mortality is high when compared with other cancers, as diagnosis often occurs during late stages. Its prevalence increased in USA over past decade and accounts for 40,000 new patients each year. Additionally, oral not fully understood likely multifactorial. To unravel relationships that are associated their virulence factors, we used 16S rDNA metagenomic...
Clinical biomarkers identified by shotgun proteomics require proteins in body fluids or tissues to be enzymatically digested before being separated and sequenced liquid chromatography-tandem mass spectrometry. How well peptide signals can resolved detected is largely dependent on the quality of sample preparation. Conventional approaches such as in-gel, in-solution, filter-based digestion, despite their extensive implementation community, become less appealing due unsatisfying...
The eukaryotic ubiquitylation machinery catalyzes the covalent attachment of small protein modifier ubiquitin to cellular target proteins in order alter their fate. Microbial pathogens exploit this post-translational modification process by encoding molecular mimics E3 ligases, enzymes that catalyze final step cascade. Here, we show Legionella pneumophila effector RavN belongs a growing class bacterial mimic host cell ligases pathway. ligase activity was located within its N-terminal region...
Pneumococcal surface protein A (PspA) and pneumococcal C (PspC, also called CbpA) are major virulence factors of Streptococcus pneumoniae (Spn). These surface-exposed choline-binding proteins (CBPs) function independently to inhibit opsonization, neutralize antimicrobial factors, or serve as adhesins. PspA PspC both carry a proline-rich domain (PRD) whose role, other than serving flexible connector between the N-terminal C-terminal domains, was up this point unknown. Herein, we demonstrate...
Abstract Brain metastasis occurs in about 50% of all women with metastatic HER2+ breast cancer and confers poor prognosis for patients. Despite effective HER2-targeted treatments peripheral trastuzumab HER2 inhibitors, limited brain permeability renders these inefficient (BCBM). The scarcity suitable patient-derived vivo models BCBM has curtailed the study molecular mechanisms that promote growth therapeutic resistance metastasis. Here, we generated characterized a luminal B cell model...
Abstract The perinucleolar compartment (PNC), a marker of genome organization / membrane-less organelle (condensate) adjacent but structurally distinct from the nucleolus, associates with metastatic behavior in cancer cells and has been used as biomarker high-throughput screening assays to identify small molecules that target metastasis. We discovered metarrestin, an investigational agent disassembles PNC multiple cell lines novel treatment for Metarrestin is currently phase I clinical...
The smoothened (Smo) receptor facilitates hedgehog signaling between kidney fibroblasts and tubules during acute injury (AKI). Tubule-derived is protective in AKI, but the role of fibroblast-selective Smo unclear. Here, we report that Smo-specific ablation reduced tubular cell apoptosis inflammation, enhanced perivascular mesenchymal cells activities, preserved function after AKI. Global proteomics these kidneys identified extracellular matrix proteins, nidogen-1 glycoprotein particular, as...
Sepsis-induced acute kidney injury (S-AKI) is the most common complication in hospitalized and critically ill patients, highlighted by a rapid decline of function occurring few hours or days after sepsis onset. Systemic inflammation elicited microbial infections believed to lead damage under immunocompromised conditions. However, although AKI has been recognized as disease with long-term sequelae, partly because associated higher risk chronic (CKD), understanding pathophysiology at molecular...
The current pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has created an urgent need to understand the pathogenesis of this infection. These efforts have been impaired lack animal models that recapitulate disease 2019 (COVID-19).
Histidine kinases serve as critical environmental sensing modules, and despite their designation simple two-component functional roles are remarkably diverse. In Agrobacterium tumefaciens pathogenesis, VirA serves with VirG the initiating sensor/transcriptional activator for inter-kingdom gene transfer transformation of higher plants. Through responses to three separate signal inputs, low pH, sugars, phenols, A. commits pathogenesis in virtually all flowering However, how these signals...
Vancomycin-intermediate Staphylococcus aureus (VISA) is associated with genetic changes that may also impact upon pathogenicity. In the current study, we compared virulence of clinical VISA strains their isogenic vancomycin-susceptible progenitors (VSSA).Production critical protein, α toxin, was assessed using Western blot analysis and correlated to agr activity a bioluminescent agr-reporter. Cytotoxicity intracellular persistence were ex vivo for VSSA within non-professional phagocytes...
The circular replication-associated protein (Rep)-encoding single-stranded (CRESS) DNA virus emergence in diverse host has been associated with severe disease. Porcine circovirus-like (Po-Circo-like [PCL] virus) is a CRESS virus, the prevalence and pathogenicity of which are rarely studied.We obtained two blood samples, four faecal intestinal samples from pig farm suffered diarrheal disease delivery room September 2020 attempted to isolate identify causative pathogen. Subsequently, only PCL...
The molecular mechanisms underlying seizure generation remain elusive, yet they are crucial for developing effective treatments epilepsy. current study shows that inhibiting c-Abl tyrosine kinase prevents apoptosis, reduces dendritic spine loss, and maintains N-methyl-d-aspartate (NMDA) receptor subunit 2B (NR2B) phosphorylated in vitro models of excitotoxicity. Pilocarpine-induced status epilepticus (SE) mice promotes phosphorylation, disrupting activity leads to fewer seizures, increases...
Polycystic ovary syndrome (PCOS) is often associated with hyperinsulinaemia and peripheral insulin resistance. Whether the resistant to a matter of controversy. The aim was therefore study effect on lactate accumulation, an indicator glucose metabolism, in granulosa–luteal cells from women PCOS normal ovarian function. were obtained undergoing clinical invitro fertilization-embryo transfer, either patients function tubal or male infertility, PCOS, without factor. down-regulated buserelin...
Fragmentation at random nucleotide locations is an essential process for preparation of DNA libraries to be used on massively parallel short-read sequencing platforms. Although instruments physical shearing, such as the Covaris S2 focused-ultrasonicator system, and products enzymatic Nextera technology NEBNext dsDNA Fragmentase kit, are commercially available, a simple inexpensive method desirable high-throughput library preparation. MspJI recently characterised restriction enzyme which...
For over a century, it has been reported that primary influenza infection promotes the development of lethal form bacterial pulmonary disease. More recently, pneumonia events caused by both viruses and bacteria have directly associated with cardiac damage. Importantly, is not known whether viral-bacterial synergy extends to extrapulmonary organs such as heart. Using label-free quantitative proteomics molecular approaches, we report pandemic A virus leads increased Streptococcus pneumoniae...
ABSTRACT The plant pathogen Agrobacterium tumefaciens expresses virulence ( vir ) genes in response to chemical signals found at the site of a wound. VirA, hybrid histidine kinase, and its cognate regulator, VirG, regulate gene expression. receiver domain carboxyl end VirA has been described as an inhibitory element because removal increased expression relative that full-length VirA. However, experiments characterized region were performed presence constitutively expressed virG . We show...