A5041545777- Lung Cancer Treatments and Mutations
- Lung Cancer Diagnosis and Treatment
- RNA modifications and cancer
- Neuroendocrine Tumor Research Advances
- Cancer Immunotherapy and Biomarkers
- Cancer Genomics and Diagnostics
- Neonatal Respiratory Health Research
- Lung Cancer Research Studies
- Neuroscience of respiration and sleep
- Radiomics and Machine Learning in Medical Imaging
- High Altitude and Hypoxia
- Congenital Diaphragmatic Hernia Studies
- Cancer-related molecular mechanisms research
- Genetic factors in colorectal cancer
- Epigenetics and DNA Methylation
- Medical Imaging and Pathology Studies
- Esophageal Cancer Research and Treatment
- Neuropeptides and Animal Physiology
- Pulmonary Hypertension Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Cancer-related gene regulation
- Neuroblastoma Research and Treatments
- Molecular Biology Techniques and Applications
- Gastric Cancer Management and Outcomes
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
Royal Liverpool and Broadgreen University Hospital NHS Trust
2008-2025
Broadgreen Hospital
2020-2025
Royal Liverpool University Hospital
2014-2023
University of Liverpool
2014-2023
Aintree University Hospitals NHS Foundation Trust
2020-2021
Massachusetts General Hospital
2020
The University of Texas MD Anderson Cancer Center
2020
Università Cattolica del Sacro Cuore
2020
Charles River Laboratories (Netherlands)
2020
John Wiley & Sons (United States)
2020
<h3>Background</h3> Lung cancer screening using low-dose CT (LDCT) was shown to reduce lung mortality by 20% in the National Screening Trial. <h3>Methods</h3> The pilot UK Cancer (UKLS) is a randomised controlled trial of LDCT for versus usual care. A population-based questionnaire used identify high-risk individuals. screen-detected nodules were managed pre-specified protocol. Cost effectiveness modelled with reference Trial reduction. <h3>Results</h3> 247 354 individuals aged 50–75 years...
BackgroundThe NLST reported a significant 20% reduction in lung cancer mortality with three annual low-dose CT (LDCT) screens and the Dutch-Belgian NELSON trial indicates similar reduction. We present results of UKLS trial.MethodsFrom October 2011 to February 2013, we randomly allocated 4 055 participants either single invitation screening LDCT or no (usual care). Eligible (aged 50–75) had risk score (LLPv2) ≥ 4.5% developing over five years. Data were collected on cases 31 December 2019...
<b>Aims and Methods:</b> A review was undertaken of 19 patients diagnosed with diffuse idiopathic pulmonary neuroendocrine cell hyperplasia (DIPNECH) between 1992 2006. <b>Results:</b> Most were women (n = 15) non-smokers 16). Clinical presentation either symptomatic disease (group 1; n 9) or as an incidental finding during investigation for another disorder, most frequently malignant 2; 10). In group 1, cough dyspnoea the frequent symptoms, average duration 8.6 years before diagnosis. Both...
PD-L1 expression on tumour cells can guide the use of anti-PD-1/PD-L1 immune modulators to treat patients with non-small cell lung cancer (NSCLC). Heterogeneity both within and between sites is a well-documented phenomenon that compromises its predictive power. Our aim was better characterise pattern extent heterogeneity view optimising sampling improve accuracy as biomarker.Expression assessed by immunochemistry using SP263 clone in 107 resected primary NSCLCs their nodal metastases....
Abstract The exceptional high mortality of lung cancer can be instigated to a degree by late diagnosis. Despite the plethora studies on potential molecular biomarkers for diagnosis, very few have reached clinical implementation. In this study, we developed panel DNA methylation and validated their diagnostic efficiency in bronchial washings from large retrospective cohort. Candidate targets previous high-throughput approaches were examined pyrosequencing an independent set 48 tumor/normal...
Biomarker tests in lung cancer have been traditionally ordered by the treating oncologist upon confirmation of an appropriate pathological diagnosis. The delay this introduces prolongs yet further what is already a complex, multi-stage, pre-treatment pathway and delays start first-line systemic treatment, which crucially informed results such analysis. Reflex testing, responsibility for testing agreed range biomarkers lies with pathologist, has shown to standardise expedite process. Twelve...
The clinical features and necropsy findings are described for seven trekkers in the Himalayas whose deaths were related to high altitude. fatal outcome was due serious pulmonary cerebral disease. Oedema of lungs brain prominent but so thrombosis haemorrhage, acute mountain sickness that have received insufficient recognition past. Most men middle aged. Some began their trekking soon after flying altitude before becoming acclimatised some remained at or climbed even higher despite development...
Abstract BACKGROUND: The UHRF1 gene possesses an essential role in DNA methylation maintenance, but its contribution to tumor suppressor hypermethylation primary human cancers currently remains unclear. METHODS: mRNA expression levels of , DNMT1 DNMT3A DNMT3B and E2F1 were evaluated 105 nonsmall cell lung carcinomas by quantitative polymerase chain reaction. status CDKN2A RASSF1 promoters was examined pyrosequencing. knocked down short hairpin RNA A549 adenocarcinoma cells. RESULTS: All 4...
During the course of a case primary pulmonary hypertension occurring in 24 year old man lung tissue became available at heart-lung transplantation 1986 and from biopsy carried out 1981. In sections showed classic plexogenic arteriopathy. 1981 they revealed migration myofibroblasts into intima lumen arteries arterioles, identification cells being confirmed by electron microscopy. five years that vascular pathology progressed to formation plexiform lesions there was an increase number...
Abstract Pulmonary endocrine cells have been studied according to their immunoreactivity for neuron‐specific enolase, gastrin‐releasing peptide (GRP), calcitonin, leucine‐enkephalin, and serotonin in the lungs of subjects with chronic bronchitis emphysema. The overall population was significantly greater comparison matched controls. change most marked lobes affected by pneumonic consolidation, did not affect equally sub‐populations as identified content peptide; difference much...
Aims: To test the reproducibility of current World Health Organization (WHO) classification thymic epithelial tumours and to determine level interobserver variation within a group pathologists, all with experience expertise in thoracic pathology. Methods results: Ninety‐five were circulated 17 pathologists UK The Netherlands over 1‐year period. Participants asked classify them according WHO criteria. diagnoses subjected statistical analysis κ values calculated. overall agreement was moderate...
Taxanes are mitotic poisons widely used in the treatment of non-small cell lung cancer (NSCLC), however, little is known about potential molecular modulators response to these compounds. Aurora B (AURKB) a critical regulator spindle assembly, previously shown overexpressed NSCLC. Here we investigated hypothesis that AURKB expression modulates efficacy taxanes NSCLC cells. mRNA was determined by qPCR 132 frozen tissues and nine lines. knocked down lines using multiple shRNA constructs....
Expression of the Notch-family ligand delta-like protein 3 (DLL3), a potential therapeutic target in small cell lung cancer (SCLC), has not been assessed real-world setting. To identify utility DLL3 as an SCLC target, we performed largest retrospective international noninterventional study to date evaluate prevalence patients.DLL3 expression was using immunohistochemistry archived histological and cytological specimens (independent paired) correlated patient demographics, clinical disease...
Abstract The lungs from 16 cases of plexogenic pulmonary arteriopathy obtained at heart‐lung transplantation, half which had primary hypertension, were examined by electron microscopy. From these the probable pathogenesis arterial intimal fibrosis in was deduced. earliest detectable change migration smooth muscle cells media; through internal elastic lamina into intima. These collected beneath endothelium and lost many their myofilaments to become myofibroblasts. They associated with ground...
To compare the morphology and antigenic profile of pulmonary neuroendocrine cells (PNECs) proliferating as a reaction to injury with those in diffuse idiopathic cell hyperplasia (DIPNECH) which carcinoids develop.The expression range antigens including markers epithelial differentiation [cytokeratins, thyroid transcription factor (TTF)-1], [neural adhesion molecule (NCAM), chromogranin, protein gene product (PGP) 9.5, neurone-specific enolase (NSE), synaptophysin], peptide products...
Aims Discriminating small‐cell lung carcinoma ( SCLC ) from large‐cell neuroendocrine LCNEC rests on morphological criteria, and reproducibility has been shown to be poor. We aimed identify immunohistochemical markers assist this diagnosis. Methods results Gene expression profiling laser captured frozen tumour samples eight tumours identified a total of 888 differentially expressed genes (DEGs), 23 which were validated by qRT–PCR. Antibodies four selected gene products then evaluated as...
Cytoglobin (CYGB) is frequently downregulated in many types of human malignancies, and its exogenous overexpression reduces proliferation cancer cells. Despite implied tumour suppressor (TSG) functions, exact role carcinogenesis remains unclear as CYGB upregulation also associated with hypoxia aggressiveness. In this study, we explore the TSG CYGB, influence on phenotype cancerous cells under stress conditions clinical significance expression promoter methylation non-small cell lung (NSCLC)....