A5005030279- Mitochondrial Function and Pathology
- Endoplasmic Reticulum Stress and Disease
- Microtubule and mitosis dynamics
- Neuroscience and Neuropharmacology Research
- Genetics and Neurodevelopmental Disorders
- Ubiquitin and proteasome pathways
- Biotin and Related Studies
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Genomics, phytochemicals, and oxidative stress
- Liver Disease Diagnosis and Treatment
- Genetic Associations and Epidemiology
- Epigenetics and DNA Methylation
- Down syndrome and intellectual disability research
- RNA Research and Splicing
- Retinoids in leukemia and cellular processes
- Environmental Toxicology and Ecotoxicology
- Drug-Induced Hepatotoxicity and Protection
- Phosphodiesterase function and regulation
- Neuroendocrine regulation and behavior
- Cardiac electrophysiology and arrhythmias
- Tryptophan and brain disorders
- Adipose Tissue and Metabolism
- Genetics, Aging, and Longevity in Model Organisms
- Ion channel regulation and function
Pohang University of Science and Technology
2019-2025
The mitochondria-associated membrane (MAM) has emerged as a cellular signaling hub regulating various processes. However, its molecular components remain unclear owing to lack of reliable methods purify the intact MAM proteome in physiological context. Here, we introduce Contact-ID, split-pair system BioID with strong activity, for identification live cells. Contact-ID specifically labeled proteins proximal contact sites endoplasmic reticulum (ER) and mitochondria, thereby identified 115...
Mitochondria-associated ER membrane (MAM) is a structure where these calcium-regulating organelles form close physical contact sites for efficient Ca2+ crosstalk. Despite the central importance of MAM dynamics in diverse biological processes, directly and specifically measuring concentrations inside technically challenging. Here, we develop MAM-Calflux, MAM-specific BRET-based indicator. The successful application bimolecular fluorescence complementation (BiFC) concept highlights...
Although large-scale genome-wide association studies (GWAS) have identified an between MAD1L1 (Mitotic Arrest Deficient-1 Like 1) and the pathology of schizophrenia, molecular mechanisms underlying this remain unclear. In present study, we aimed to address these by examining role MAD1 (the gene product MAD1L1) in key neurodevelopmental processes mice human organoids. Our findings indicated that is highly expressed during active cortical development deficiency leads impairments neuronal...
Abstract Identifying proteins at organelle contact sites, such as mitochondria-associated endoplasmic reticulum membranes (MAM), is essential for understanding vital cellular processes, yet challenging due to their dynamic nature. Here we report “OrthoID”, a proteomic method utilizing engineered enzymes, TurboID and APEX2, the biotinylation (Bt) adamantylation (Ad) of close mitochondria (ER), respectively, in conjunction with high-affinity binding pairs, streptavidin-biotin (SA-Bt)...
The neurodevelopmental theory of schizophrenia emphasizes early brain development in its etiology. Genome-wide association studies have linked to genetic variations AS3MT (arsenite methyltransferase) gene, particularly the increased expression d2d3 isoform. To investigate biological basis this with pathophysiology, we established a transgenic mouse model (AS3MT -Tg) ectopically expressing at cortical neural stem cells. -Tg mice exhibited enlarged ventricles and deficits sensorimotor gating...
The endoplasmic reticulum (ER) and mitochondria form a unique subcellular compartment called mitochondria-associated ER membranes (MAMs). Disruption of MAMs impairs Ca2+ homeostasis, triggering pleiotropic effects in the neuronal system. Genome-wide kinase-MAM interactome screening identifies casein kinase 2 alpha 1 (CK2A1) as regulator composition transport MAMs. CK2A1-mediated phosphorylation PACS2 at Ser207/208/213 facilitates MAM localization CK2A1-PACS2-PKD2 complex, regulating...
Neuronal morphogenesis requires multiple regulatory pathways to appropriately determine axonal and dendritic structures, thereby enable the functional neural connectivity. Yet, however, precise mechanisms components that regulate neuronal are still largely unknown. Here, we newly identified sequential phosphorylation of NDEL1 critical for through human kinome screening phospho-proteomics analysis from mouse brain lysate. DYRK2 phosphorylates S336 prime S332 by GSK3β. TARA, an interaction...
Abstract Mitochondrial movement in neurons is finely regulated to meet the local demand for energy and calcium buffering. Elaborate transport machinery including motor complexes required deliver localize mitochondria appropriate positions. Defects mitochondrial are associated with various neurological disorders without a detailed mechanistic information. In this study, we present evidence that dystrobrevin-binding protein 1 (dysbindin), schizophrenia-associated factor, plays critical role...
Regulation of microtubule dynamics is required to properly control various steps neurodevelopment. In this study, we identified granule cell antiserum-positive 14 (Gcap14) as a plus-end-tracking protein and regulator during Gcap14 knockout mice exhibited impaired cortical lamination. deficiency resulted in defective neuronal migration. Moreover, nuclear distribution element nudE-like 1 (Ndel1), an interacting partner Gcap14, effectively corrected the downregulation defects migration caused...
Abstract A comprehensive understanding of isoniazid (INH)-mediated hepatotoxic effects is essential for developing strategies to predict and prevent severe liver toxicity in tuberculosis treatment. Our study utilized multi-omics profiling investigate the toxic INH, revealing significant involvement endoplasmic reticulum (ER) stress, mitochondrial impairment, redox imbalance, altered metabolism. Followed-up mechanistic studies revealed that INH triggered generation cytosolic reactive oxygen...
Disrupted-in-schizophrenia 1 (DISC1) is a scaffold protein that has been implicated in multiple mental disorders. DISC1 known to regulate neuronal proliferation, signaling, and intracellular calcium homeostasis, as well neurodevelopment. Although was linked sleep-associated behaviors, whether functions the circadian rhythm not determined yet. In this work, we revealed Disc1 expression exhibits daily oscillating pattern regulated by binding of locomotor output cycles kaput (CLOCK) Brain...
Dendritic spines are the central postsynaptic machinery that determines synaptic function. The F-actin within dendritic regulates their dynamic formation and elimination. Rai14 is an F-actin-regulating protein with a membrane-shaping Here, we identified roles of for regulation spine dynamics associated stress-induced depressive-like behaviors. Rai14-deficient neurons exhibit reduced density in Rai14+/- mouse brain, resulting impaired functional activity. was protected from degradation by...