A5016947629- Connexins and lens biology
- Tendon Structure and Treatment
- Urinary Bladder and Prostate Research
- Advanced Electron Microscopy Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Shoulder Injury and Treatment
- Advanced Glycation End Products research
- Mitochondrial Function and Pathology
- Tissue Engineering and Regenerative Medicine
- Malaria Research and Control
- Ion channel regulation and function
- Ion Channels and Receptors
- Metabolism and Genetic Disorders
- Cellular Mechanics and Interactions
- Neuroscience and Neuropharmacology Research
- Bone Tissue Engineering Materials
- Retinal Diseases and Treatments
- Extracellular vesicles in disease
- Shoulder and Clavicle Injuries
- Mosquito-borne diseases and control
- Ion Transport and Channel Regulation
- Retinal and Macular Surgery
- Intraocular Surgery and Lenses
- Electron and X-Ray Spectroscopy Techniques
- Mesenchymal stem cell research
Kurume University
2015-2024
Ube (Japan)
2019
Kyushu University
1995-2011
Case Western Reserve University
1991-1994
University School
1994
Western University of Health Sciences
1992
Abstract —Mitochondria are one of the enzymatic sources reactive oxygen species (ROS) and could also be a major target for ROS-mediated damage. We hypothesized that ROS may induce mitochondrial DNA (mtDNA) damage, which leads to defects mtDNA-encoded gene expression respiratory chain complex enzymes thus contribute progression left ventricular (LV) remodeling failure after myocardial infarction (MI). In murine model MI created by anterior descending coronary artery ligation 4 weeks, LV was...
Loss of mitochondrial membrane potential (ΔΨm) triggers dramatic structural changes in mitochondria from a tubular to globular shape, referred as fragmentation; the resulting are called swelled or ring/doughnut mitochondria. We evaluated early period during ΔΨm loss-induced transformation after carbonyl cyanide m-chlorophenyl hydrazine (CCCP) administration using newly developed correlative microscopic method combined with fluorescence live imaging and volume electron microscopy. found that...
The superoxide‐producing NAD(P)H oxidase Nox4 was initially identified as an enzyme that is highly expressed in the kidney and possibly involved oxygen sensing cellular senescence. Although also abundant vascular endothelial cells, its role remains to be elucidated. Here we show preferentially localizes nucleus of human umbilical vein cells (HUVECs), by immunocytochemistry immunoelectron microscopy using three kinds affinity‐purified antibodies raised against distinct immunogens from Nox4....
Block-face imaging is a scanning electron microscopic technique which enables easier acquisition of serial ultrastructural images directly from the surface resin-embedded biological samples with similar quality to transmission micrographs. In present study, we analyzed three-dimensional architecture podocytes using block-face imaging. It was previously believed that are divided into three kinds subcellular compartment: cell body, primary process, and foot simply aligned in this order. When...
Abstract Zebrafish have a characteristic horizontal‐stripe pigment pattern made by specific distribution of three types cells: melanophores, xanthophores, and iridophores. This is valuable model to investigate how the spatial patterns form during animal development. Although recent findings suggest that interactions among cells play key role, particular details these not yet been clarified. In this report, we performed transmission electron microscopic study show distribution, conformation,...
The generation of reactive oxygen species (ROS) is enhanced in the failing myocardium. We hypothesized that ROS were also increased limb skeletal muscles heart failure. Methods and Results-- Myocardial infarction (MI) was created mice by ligating left coronary artery. After 4 weeks, ventricle dilated contractility diminished echocardiography. Left ventricular end-diastolic pressure elevated after MI association with an increase lung weight/body weight presence pleural effusion. muscles,...
Abstract The orderly pigment pattern of zebrafish ( Danio rerio ) is a good model system for studying how spatial patterns form in animals. Recent molecular genetic studies have shown that interactions between the cells play major roles formation. In present study, we performed comparative transmission electron microscopy cells, order to clarify structural tissues with and without striped pattern. patterned tissues, were distributed as one‐cell‐thick sheet. layer sheets always kept strictly....
There is growing evidence that the subacromial injection of hyaluronic acid (HA) effective for pain relief in rotator cuff tears; however, its effect on tendon-to-bone healing remains unknown.To examine HA chondrogenesis mesenchymal stem cells (MSCs) vitro and a repair model.Controlled laboratory study.Bilateral complete tears infraspinatus tendon were made rabbits subsequently repaired. Before closure, 1 mL was applied to repaired site, phosphate-buffered saline used opposite side as...
We recently demonstrated that Rho-kinase, an effector of the small GTPase Rho, is substantially involved in pathogenesis arteriosclerosis. In this study, we examined whether Rho-kinase also in-stent restenosis and if so, what mechanism involved.Pigs underwent stent implantation left coronary artery with or without administration fasudil (30 mg/kg per day orally), a specific inhibitor, starting 2 days before procedure for duration 4 weeks. On 28, reductions diameter neointimal formation...
To obtain insights into the cardiomyogenic potential of hematopoietic tissue, we intravenously (i.v.) injected purified stem/progenitor cells newborn recipients that may fully potentiate developmental plasticity stem cells. Transplantation mouse bone marrow (BM) lineage antigen-negative (Lin-) resulted in generation displayed cardiomyocyte-specific antigenic profiles and contractile function when transplanted syngeneic recipients. clarify mechanism underlying potential, green fluorescent...
Abstract Successful rotator cuff repair requires biological anchoring of the repaired tendon to bone. However, histological structure tendon-bone interface differs from that a normal insertion. We analysed differences between insertion and after surgery in mechanical properties, histomorphometric analysis 3-dimensional ultrastructure cells using rat model. Twenty-four adult Sprague-Dawley (SD) rats underwent complete tear subsequent supraspinatus tendon. The was evaluated at 4, 8 12 weeks...
At the presynaptic active zone (AZ), related cytomatrix proteins CAST and ELKS organize release machinery. While is known to regulate AZ size neurotransmitter release, role of integral system CAST/ELKS together poorly understood. Here, we show that have both redundant unique roles in coordinating synaptic development, function, maintenance retinal photoreceptor ribbon synapses. A double knockout (dKO) mouse showed high levels ectopic synapses reduced responses visual stimulation. Ectopic...
Blockade of the renin-angiotensin system improves impaired endothelium-dependent relaxations associated with hypertension and aging, partly through amelioration endothelium-derived hyperpolarizing factor (EDHF)-mediated responses. Although nature EDHF is still controversial, recent studies have suggested involvement gap junctions in EDHF-mediated Gap consist connexins (Cx), we therefore tested whether expression Cx vascular endothelial cells would be altered by antihypertensive treatment....
Interaction between advanced glycation end-products (AGEs) and their receptor (RAGE) plays a central role in diabetic nephropathy pathogenesis. Pathophysiological crosstalk the AGEs–RAGE system angiotensin II (Ang II) is also involved this disease. This study investigated of proliferator-activated receptor-γ (PPAR-γ)-modulating activity on inhibition monocyte chemoattractant protein (MCP-1) expression. Telmisartan, an Ang type 1 blocker, downregulated RAGE mRNA inhibited superoxide...