A5084407985- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- Histone Deacetylase Inhibitors Research
- Autophagy in Disease and Therapy
Guangzhou Institutes of Biomedicine and Health
2017-2025
Chinese Academy of Sciences
2017-2025
University of Hong Kong
2021-2025
University of Chinese Academy of Sciences
2025
Guangzhou Medical University
2017-2021
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2020-2021
Abstract Pluripotent stem cells hold great promise in regenerative medicine and developmental biology studies. Mitochondrial metabolites, including tricarboxylic acid (TCA) cycle intermediates, have been reported to play critical roles pluripotency. Here we show that TCA enzymes Pdha1, Pcb, Aco2, Cs, Idh3a, Ogdh, Sdha Mdh2 are translocated the nucleus during somatic cell reprogramming, primed-to-naive transition totipotency acquisition. The nuclear-localized Idh3a promote reprogramming...
Induced pluripotent stem cells (iPSCs) have fewer and immature mitochondria than somatic mainly rely on glycolysis for energy source. During cell reprogramming, other organelles get remodeled. However, events of organelle remodeling interaction during reprogramming not been extensively explored. We show that both SKP/SKO (Sox2, Klf4, Pou5f1/Oct4) SKPM/SKOM (SKP/SKO plus Myc/c-Myc) lead to decreased mitochondrial mass but with different kinetics by divergent pathways. Rapid, MYC/c-MYC-induced...
Remodeled mitochondria are characteristic of pluripotent stem cells. However, a role for mitochondrial movement and distribution in pluripotency remains unknown. Here, we show that retrograde transport-mediated perinuclear clustering via dynein complex occurs at the early phase induction. Interestingly, this redistribution is regulated by Yamanaka factor OCT4 but not SOX2 or KLF4. This redistribution, which has effect on efficiency somatic cell reprogramming, also depends DRP1-mediated...
Somatic cells can be reprogrammed into induced pluripotent stem (iPSCs) by defined factors. Here, we describe a protocol for imaging mitochondrial permeability transition pore (mPTP) opening in reprogramming of somatic using confocal microscope. We also method to sort high and low mPTP calcein fluorescence reprogram these sorted iPSCs. These protocols are suitable uncovering the mechanisms function other cell fate conversions. For complete details on use execution this protocol, please refer...