A5028756404- Pluripotent Stem Cells Research
- Mitochondrial Function and Pathology
- CRISPR and Genetic Engineering
- Pharmacological Effects and Toxicity Studies
- Autophagy in Disease and Therapy
- Epigenetics and DNA Methylation
- Insect Resistance and Genetics
- Renal Transplantation Outcomes and Treatments
- Parkinson's Disease Mechanisms and Treatments
- Genetic Neurodegenerative Diseases
- RNA modifications and cancer
- Mosquito-borne diseases and control
- Genetics, Aging, and Longevity in Model Organisms
- Histone Deacetylase Inhibitors Research
- Metabolism and Genetic Disorders
- Cancer-related molecular mechanisms research
- Cell death mechanisms and regulation
- Tissue Engineering and Regenerative Medicine
- Viral Infectious Diseases and Gene Expression in Insects
- Mycobacterium research and diagnosis
- Molecular Biology Techniques and Applications
- Endoplasmic Reticulum Stress and Disease
- Entomopathogenic Microorganisms in Pest Control
Chinese Academy of Sciences
2015-2025
Guangzhou Institutes of Biomedicine and Health
2015-2025
Guangzhou Medical University
2017-2025
University of Hong Kong
2018-2025
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2018-2022
Third Affiliated Hospital of Guangzhou Medical University
2022
Sun Yat-sen University
2013-2016
University of Science and Technology of China
2016
TGF-β plays a central role in mediating epithelial-mesenchymal transition (EMT) by activating the Smad pathway. In addition, accumulating evidence suggests that TGF-β-induced EMT is NF-κB-dependent various cancer types. However, it largely unclear if NF-κB mediates breast cancer, and this mediation occurs, regulatory mechanisms are unknown. our study, we found activates Inhibition of signaling markedly abrogates EMT. By studying mechanism signaling, lncRNA NKILA was upregulated essential for...
Valproic acid (VPA) is widely used to treat epilepsy, migraine, chronic headache, bipolar disorder, and as adjuvant chemotherapy, but potentially causes idiosyncratic liver injury. Alpers‐Huttenlocher syndrome (AHS), a neurometabolic disorder caused by mutations in mitochondrial DNA polymerase gamma (POLG), associated with an increased risk of developing fatal VPA hepatotoxicity. However, the mechanistic link this clinical mystery remains unknown. Here, fibroblasts from 2 AHS patients were...
Mitochondrial quality control plays an important role in maintaining mitochondrial homeostasis and function. Disruption of degrades brain We found that flunarizine (FNZ), a drug whose chronic use causes parkinsonism, led to parkinsonism-like motor dysfunction mice. FNZ induced decreased mass specifically the brain. content both neurons astrocytes, without affecting number nigral dopaminergic neurons. In human neural progenitor cells, also depletion. Mechanistically, independent ATG5- or...
Induced pluripotent stem cells (iPSCs) have fewer and immature mitochondria than somatic mainly rely on glycolysis for energy source. During cell reprogramming, other organelles get remodeled. However, events of organelle remodeling interaction during reprogramming not been extensively explored. We show that both SKP/SKO (Sox2, Klf4, Pou5f1/Oct4) SKPM/SKOM (SKP/SKO plus Myc/c-Myc) lead to decreased mitochondrial mass but with different kinetics by divergent pathways. Rapid, MYC/c-MYC-induced...
Remodeled mitochondria are characteristic of pluripotent stem cells. However, a role for mitochondrial movement and distribution in pluripotency remains unknown. Here, we show that retrograde transport-mediated perinuclear clustering via dynein complex occurs at the early phase induction. Interestingly, this redistribution is regulated by Yamanaka factor OCT4 but not SOX2 or KLF4. This redistribution, which has effect on efficiency somatic cell reprogramming, also depends DRP1-mediated...
The mechanisms of somatic cell reprogramming have been revealed at multiple levels. However, the lack tools to monitor different reactive oxygen species (ROS) has left their distinct signals and roles in unknown. We hypothesized that mitochondrial flashes (mitoflashes), recently identified spontaneous bursts superoxide signaling, play a role reprogramming. Here we show frequency mitoflashes transiently increases, accompanied by flash amplitude reduction, during early stages This transient...
Summary Background Neuritic degeneration is an important early pathological step in neurodegeneration. Aim The purpose of this study was to explore the mechanisms connecting neuritic functional and morphological remodeling endoplasmic reticulum ( ER ) mitochondria. Methods Here, we set up models by neurite cutting‐induced neural human‐induced pluripotent stem cell‐derived neurons. Results We found that becomes fragmented forms complexes with mitochondria, which induces IP 3R‐dependent...
Abstract As baculoviruses usually have a narrow insecticidal spectrum, knowing the mechanisms by which they control host-range is prerequisite for improvement of their applications as pesticides. In this study, from supernatant culture cells transfected with DNAs an Autographa californica multiple nucleopolyhedrovirus (AcMNPV) mutant lacking antiapoptotic gene p35 (vAc ∆P35 ) and cosmid representing fragment Spodoptera exigua (SeMNPV), viral strain was plaque-purified named vAcRev. vAcRev...
Sf-caspase-1 is the principal effector caspase in Spodoptera frugiperda cells. Like caspases other organisms, processed by upstream to form an active heterotetramer composed of p19 and p12 subunits. The regulation crucial for cellular viability. In mammal cells, subunits caspase-3 were rapidly degraded relative its proenzyme form. present study, S. Sf9 cells transiently transfected with plasmids encoding different fragments Sf-caspase-1: pro-Sf-caspase-1 (p37), a prodomain deleted fragment...
Somatic cells can be reprogrammed into induced pluripotent stem (iPSCs) by defined factors. Here, we describe a protocol for imaging mitochondrial permeability transition pore (mPTP) opening in reprogramming of somatic using confocal microscope. We also method to sort high and low mPTP calcein fluorescence reprogram these sorted iPSCs. These protocols are suitable uncovering the mechanisms function other cell fate conversions. For complete details on use execution this protocol, please refer...
Valproic acid (VPA) is a widely used antiepileptic drug to treat epilepsy and psychiatric disorders, but potentially causes idiosyncratic liver injury. Alpers-Huttenlocher syndrome (AHS), neurometabolic disorder caused by mutations in mitochondrial DNA polymerase gamma (POLG), associated with an increased risk of developing fatal VPA hepatotoxicity. However, the mechanistic link this clinical mystery remains unknown. Here, we established induced pluripotent stem cell （iPSC） toxicity model...