A5045941650- Renal Diseases and Glomerulopathies
- Renal and related cancers
- Chronic Kidney Disease and Diabetes
- Renin-Angiotensin System Studies
- Biomedical Research and Pathophysiology
- Nitric Oxide and Endothelin Effects
- Apelin-related biomedical research
- Single-cell and spatial transcriptomics
- Hormonal Regulation and Hypertension
- Birth, Development, and Health
- Genetic and Kidney Cyst Diseases
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Sleep and Work-Related Fatigue
- Parathyroid Disorders and Treatments
- Sodium Intake and Health
- Tissue Engineering and Regenerative Medicine
- Circadian rhythm and melatonin
- Systemic Lupus Erythematosus Research
- Platelet Disorders and Treatments
- Ion Transport and Channel Regulation
- Photoreceptor and optogenetics research
- Congenital Heart Disease Studies
- Receptor Mechanisms and Signaling
- Barrier Structure and Function Studies
University of Southern California
2017-2024
Johns Hopkins University
2018
Northport VA Medical Center
2017
Stony Brook University
2017
Texas Children's Hospital
2017
Baylor College of Medicine
2017
University of California, Los Angeles
2017
Center for Research in Environmental Epidemiology
2016
Barcelona Institute for Global Health
2016
Bajcsy-Zsilinszky Kórház és Rendelőintézet
2007
Tissue regeneration is limited in several organs including the kidney, contributing to high prevalence of kidney disease globally. However, evolutionary and physiological adaptive responses presence renal progenitor cells suggest existing remodeling capacity. This study uncovered endogenous tissue mechanisms that were activated by loss body fluid salt regulated a unique niche minority cell type called macula densa (MD). Here we identified neuronal differentiation features MD sense local...
The vasoconstrictor effects of angiotensin II via type 1 receptors in vascular smooth muscle cells are well established, but the direct on endothelial (VECs) vivo and mechanisms how VECs may mitigate II-mediated vasoconstriction not fully understood. present study aimed to explore molecular pathophysiological relevance actions kidney brain microvessels vivo.
Rationale: Lymphatic vessels function to drain interstitial fluid from a variety of tissues. Although shear stress generated by flow is known trigger lymphatic expansion and remodeling, the molecular basis underlying flow-induced growth unknown. Objective: We aimed gain better understanding mechanism which laminar activates proliferation. Methods Results: Primary endothelial cells dermal blood (blood vascular [LECs]) were exposed low-rate steady flow. Shear stress–induced cellular responses...
The prorenin receptor (PRR) was originally proposed to be a member of the renin-angiotensin system (RAS); however, recent work questioned their association. present paper describes functional link between PRR and RAS in renal juxtaglomerular apparatus (JGA), classic anatomical site RAS. expression found sensory cells JGA, macula densa (MD), immunohistochemistry-localized MD basolateral cell membrane mouse, rat, human kidneys. activation led MAP kinase ERK1/2 signaling stimulation PGE2...
Lupus nephritis (LN) is a major organ complication and cause of morbidity mortality in patients with systemic lupus erythematosus (SLE). There an unmet medical need for developing more efficient specific, mechanism-based therapies, which depends on improved understanding the underlying LN pathogenesis. Here we present direct visual evidence from high-power intravital imaging local kidney tissue microenvironment mouse models showing that activated memory T cells originated immune organs...
Endothelial cells are important in the maintenance of healthy blood vessels and development vascular diseases. However, origin dynamics endothelial precursors remodeling at single-cell level have been difficult to study vivo owing technical limitations. Therefore, we aimed develop a direct visual approach track fate function single over several days weeks same bed using multiphoton microscopy (MPM) transgenic Cdh5-Confetti mice kidney glomerulus as model. Individual lineage were identified...
Although macula densa (MD) cells are chief regulatory in the nephron with unique microanatomical features, they have been difficult to study full detail due their inaccessibility and limitations earlier microscopy techniques. The present used a new mouse model comprehensive imaging approach visualize so far unexplored features of MD cells, regulation, functional relevance. MD-GFP mice conditional partial induction green fluorescent protein (GFP) expression, which specifically intensely...
Abstract Chronic and genetic kidney diseases such as autosomal dominant polycystic disease (ADPKD) have few therapeutic options, clinical trials testing small molecule drugs been unfavorable due to low bioavailability adverse side effects. Although nanoparticles can be designed deliver directly the diseased site, there are no kidney-targeted nanomedicines clinically available, most FDA-approved administered intravenously which is not ideal for chronic diseases. To meet these challenges of...
<h3>Objectives</h3> Night shift work has been classified as a probable human carcinogen by the International Agency for Research on Cancer, based experimental studies and limited evidence breast cancer risk. Evidence at other sites is scarce. We evaluated association between night stomach risk in population-based case–control study. <h3>Methods</h3> A total of 374 incident adenocarcinoma cases 2481 population controls were included from MCC-Spain Detailed data lifetime collected including...
Podocyte calcium (Ca2+) signaling plays important roles in the (patho)physiology of glomerular filtration barrier. Overactivation podocyte transient receptor potential canonical (TRPC) channels including TRPC6 and purinergic via P2 receptors that are known mechanosensors can increase intracellular Ca2+ levels ([Ca2+]i) cause cell injury, proteinuria disease diabetes. However, mechanistic details trigger activation these pathways vivo intact environment lacking. Here we show direct visual...
Alport syndrome (AS) is a genetic disorder caused by mutations in type IV collagen that lead to defective glomerular basement membrane, filtration barrier (GFB) damage, and progressive chronic kidney disease. While the basis of AS well known, molecular cellular mechanistic details disease pathogenesis have been elusive, hindering development mechanism-based therapies. Here, we performed intravital multiphoton imaging local tissue microenvironment X-linked mouse model directly visualize major...
Dual endothelin-1 (ET-1) and angiotensin II (AngII) receptor antagonism with sparsentan has strong antiproteinuric actions via multiple potential mechanisms that are more pronounced, or additive, compared current standard of care using blockers (ARBs). Considering the many ET-1 AngII on cell types, this study aimed to determine glomeruloprotective ARB losartan by direct visualization its effects in intact kidney focal segmental glomerulosclerosis (FSGS) intravital multiphoton microscopy. In...
This study discovered the high rate of protein synthesis in macula densa (MD) cells by applying direct imaging techniques with single cell resolution. Physiological activation and mammalian target rapamycin signaling played important regulatory roles this process. new feature is a novel component tubuloglomerular cross talk glomerular hemodynamic functions MD cells. Future work needed to elucidate nature (patho)physiological role specific proteins synthesized
Significance Statement The functional significance of renomedullary interstitial cells, which are uniquely and abundantly expressed in the renal inner medulla, is largely unknown. In vitro studies have demonstrated that endothelin A receptors regulate multiple aspects cell function. Using a novel mouse model with inducible cell–specific receptor gene targeting, authors found compared control mice, mice lacking cells exhibited reduced BP, enhanced natriuresis diuresis, increased endogenous...
Vascular cognitive impairment (VCI) is a growing health problem in many developed countries due to aging of its population. Aging-induced neurovascular coupling (NVC) responses and brain microvascular rarefaction have been implicated as major driving mechanisms age-related decline. One the cellular hallmarks NVC involve release vasodilator nitric oxide (NO) by endothelial cells response mediators released from activated neurons. Recent studies our group identified characterized novel,...
The control of whole-body energy metabolism involves intricate crosstalk between multiple organ systems including the gut and brain that play well-recognized roles in this process. However, important contributions mechanisms by other organs are poorly understood. Errors metabolic regulation can lead to human diseases diabetes obesity affect 75% population significant comorbidities. Improving our mechanistic understanding multi-organ health disease is essential for developing highly effcient...
Macula densa (MD) cells are major regulators of nephron, kidney, and whole body functions, including the control renal blood flow, glomerular filtration rate (GFR), renin release thus maintenance fluid electrolyte balance. Our recent studies identified high MD protein synthesis regulated by mTORC1 complex mechanistic target rapamycin (mTOR) as a novel component cell hemodynamic regulatory functions. Raptor is an essential player in mTORC1’s key roles to regulate energy homeostasis, growth,...