A5043391533- Immune Cell Function and Interaction
- HIV Research and Treatment
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- HIV/AIDS Research and Interventions
- Immunotherapy and Immune Responses
- HIV-related health complications and treatments
- Immunodeficiency and Autoimmune Disorders
- Transplantation: Methods and Outcomes
- Congenital Heart Disease Studies
- Cytomegalovirus and herpesvirus research
- Food Allergy and Anaphylaxis Research
- Cardiac Arrhythmias and Treatments
- Cardiac pacing and defibrillation studies
- CAR-T cell therapy research
- Animal Virus Infections Studies
- Renal Transplantation Outcomes and Treatments
- Diabetes and associated disorders
- Xenotransplantation and immune response
- Cardiac Valve Diseases and Treatments
- Tryptophan and brain disorders
- Long-Term Effects of COVID-19
- Atrial Fibrillation Management and Outcomes
- Neuroinflammation and Neurodegeneration Mechanisms
Hospital General Universitario Gregorio Marañón
2016-2025
Centre for Genomic Regulation
2023
Translational Research in Oncology
2023
Clínica CES
2017-2022
Mayo Clinic in Arizona
2018
Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul
2016
Instituto Federal Farroupilha
2016
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2016
Boston Children's Hospital
2012-2015
Harvard University
2014-2015
Melanoma is one of the most aggressive cancers, and its incidence increasing. These tumors derive from melanocyte lineage remain incurable after metastasis. Here we report that SONIC HEDGEHOG (SHH)-GLI signaling active in matrix human hair follicles, it required for normal proliferation melanocytes culture. SHH-GLI also regulates survival melanomas: growth, recurrence, metastasis melanoma xenografts mice are prevented by local or systemic interference HH-GLI function. Moreover, show...
The rapid development of mRNA-based vaccines against the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) led to design accelerated vaccination schedules that have been extremely effective in naive individuals. While a two-dose immunization regimen with BNT162b2 vaccine has demonstrated provide 95% efficacy individuals, effects second dose individuals who previously recovered from natural SARS-CoV-2 infection not investigated detail. In this study, we characterize spike-specific...
Regulatory T cells (Treg) are essential for immune balance, preventing overreactive responses and autoimmunity. Although traditionally characterized as CD4+CD25+CD127 low FoxP3 hi , recent research has revealed diverse Treg subsets such Tr1, Tr1-like, CD8 Treg. dysfunction leads to severe autoimmune diseases immune-mediated inflammatory disorders. Inborn errors of immunity (IEI) a group disorders that affect correct functioning the system. IEI include Tregopathies caused by genetic mutations...
Background/Objectives: Decompensated cirrhosis is characterized by systemic inflammation and innate adaptive immune dysfunction. Hepatic encephalopathy (HE) a prevalent debilitating condition cognitive disturbances in which ammonia play synergistic pathogenic role. Extraskeletal functions of vitamin D include immunomodulation, its deficiency has been implicated dysfunction different forms impairment. The aim was to assess changes function decompensated patients with receiving...
HIV-1 infects immature dendritic cells (iDCs), but infection is inefficient compared with activated CD4+ T and only involves a small subset of iDCs. We analyzed whether this could be attributed to specific cellular restrictions during the viral life cycle. To study env-independent restriction infection, we used single-round assay pseudotyped vesicular stomatitis virus G protein (HIV-VSVG). Small interfering RNA–mediated depletion APOBEC3G/3F (A3G/3F), not TRIM5α, enhanced iDCs, indicating...
Abstract Chimeric antigen receptor (CAR) T cell–related HLH/MAS is an unusual manifestation of severe cytokine release syndrome (CRS) with poor prognosis and a challenging diagnosis. The establishment specific diagnosis criteria essential, the combination several techniques for CAR T‐cell follow‐up, allows more precise management this complication.
Regulatory T cells (Tregs) play an important role in infections modulating host immune responses and avoiding overreactive immunity. The mechanisms underlying their action HIV-infected patients have not been well established. HIV can infect Treg, but little is known about the effects of infection on Treg phenotype function. objective this study was to investigate whether in-vitro modifies suppressive capacity cells.Because are a subset CD4 cells, could produce alterations methylation pattern...
The ability to identify and ablate different arrhythmia mechanisms after the total cavopulmonary connection has not been studied in detail.After obtaining Institutional Review Board approval according institutional guidelines, consecutive patients a undergoing electrophysiology study over 6-year period were included (2006-2012). Arrhythmia mechanism was determined, procedural outcome defined as complete, partial success, or failure. A 12-point severity score calculated for each patient at...
In Fontan and atrial switch patients, transcatheter ablation is limited by difficult access to the pulmonary venous atrium. recent years, transbaffle (TBA) has been described, but data document its safety utility.All ablative electrophysiological study cases of this population performed between January 2006 December 2010 at Boston Children's Hospital were reviewed. Pre-case follow-up clinical characteristics documented. Adverse events classified severity attributability intervention. We...
SARS-CoV-2 has infected more than 200 million people worldwide, with 4 associated deaths. Although 80% of develop asymptomatic or mild COVID-19, can induce a profound dysregulation the immune system. Therefore, it is important to investigate whether clinically recovered individuals present sequelae. The potential presence long-term system could constitute risk factor for re-infection and development other pathologies. Here, we performed deep analysis in 35 COVID-19 previously compared 16...
SARS-CoV-2 emerged as a new coronavirus causing COVID-19, and it has been responsible for more than 760 million cases 6.8 deaths worldwide until March 2023. Although infected individuals could be asymptomatic, other patients presented heterogeneity wide range of symptoms. Therefore, identifying those being able to classify them according their expected severity help target health efforts effectively.Therefore, we wanted develop machine learning model predict who will severe disease at the...
Failure of anti-TNF therapy is a real concern in children with inflammatory bowel disease (IBD) owing to the limited therapeutic arsenal. Anti-TNF drugs modulate immune response, key driver chronic inflammation IBD. Accordingly, we analyzed changes frequency T-lymphocyte and cytokine levels after 6 weeks treatment identify potential biomarkers response drugs. We recruited 77 patients under 18 years age diagnosed IBD treated an drug. Using flow cytometry multiplex ELISA, 31 populations four...
Abstract CD25, the alpha chain of IL-2 receptor, is expressed on activated effector T cells that mediate immune graft damage. Induction immunosuppression commonly used in solid organ transplantation and can include antibodies blocking CD25. However, regulatory (Tregs) also rely CD25 for their proliferation, survival, function. Therefore, CD25-blockade may compromise Treg protective role against rejection. We analysed vitro effect basiliximab (BXM) viability, phenotype, proliferation cytokine...
The development of mRNA vaccines represented a significant achievement in response to the global health crisis during SARS-CoV-2 pandemic. Evaluating vaccine efficacy entails identifying different anti-SARS-CoV-2 antibodies, such as total antibodies against Receptor Binding Domain (RBD) S-protein, or neutralizing (NAbs). This study utilized an innovative PETIA-based kit measure NAb, and investigation aimed assess whether levels anti-RBD IgG NAb uniformly measured 30 days after vaccination...
In the context of Covid-19 pandemic, fast development vaccines with efficacy around 95% preventing illness provides a unique opportunity to reduce mortality associated pandemic. However, in absence efficacious prophylactic medications and few treatments for this infection, induction robust protective immunity is required effective disease control, not only prevent but also infection shedding/transmission. The objective our study was analyze level specific humoral cellular T-cell responses...
Due to their suppressive capacity, regulatory T cells (Tregs) have attracted growing interest as an adoptive cellular therapy for the prevention of allograft rejection, but limited Treg recovery and lower quality adult-derived Tregs could represent obstacle success. To address this challenge, we developed a new approach that provides large quantities with high purity excellent features, sourced from thymic tissue routinely removed during pediatric cardiac surgeries (thyTregs). We report on...