A5054910534- HIV Research and Treatment
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- RNA Interference and Gene Delivery
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Dendrimers and Hyperbranched Polymers
- Animal Virus Infections Studies
- Transplantation: Methods and Outcomes
- Renal Transplantation Outcomes and Treatments
- Virus-based gene therapy research
- Antimicrobial Peptides and Activities
- CAR-T cell therapy research
- HIV/AIDS drug development and treatment
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Food Allergy and Anaphylaxis Research
- Virology and Viral Diseases
- Advanced biosensing and bioanalysis techniques
- Xenotransplantation and immune response
- Click Chemistry and Applications
- Immunodeficiency and Autoimmune Disorders
- HIV/AIDS Research and Interventions
- vaccines and immunoinformatics approaches
Hospital General Universitario Gregorio Marañón
2015-2025
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2010-2016
Bentham Science Publishers (China)
2014
Bentham Science Publishers (United Arab Emirates)
2014
Instituto de Salud Carlos III
2010
University Hospital of Geneva
2004-2007
Inserm
2001-2006
Dendritic cells (DCs) are essential components of the early events HIV infection. Here, we characterized trafficking pathways that HIV‐1 follows during its capture by DCs and subsequent presentation to CD4 + T via an infectious synapse. Immunofluorescence microscopy indicates virus‐containing compartment in mature (mDCs) co‐labels for tetraspanins CD81, CD82, CD9 but contains little CD63 or LAMP‐1. Using ratio imaging pH‐reporting fluorescent virions live DCs, show is internalized...
Dendritic cells (DCs) are essential for the early events of human immunodeficiency virus (HIV) infection. Model systems HIV sexual transmission have shown that DCs expressing DC-specific C-type lectin DC-SIGN capture and internalize at mucosal surfaces efficiently transfer to CD4+ T in lymph nodes, where viral replication occurs. Upon DC–T cell clustering, internalized accumulates on DC side contact zone (infectious synapse), between cells, whereas receptors coreceptors enriched side. Viral...
The rapid development of mRNA-based vaccines against the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) led to design accelerated vaccination schedules that have been extremely effective in naive individuals. While a two-dose immunization regimen with BNT162b2 vaccine has demonstrated provide 95% efficacy individuals, effects second dose individuals who previously recovered from natural SARS-CoV-2 infection not investigated detail. In this study, we characterize spike-specific...
HIV-1 infects immature dendritic cells (iDCs), but infection is inefficient compared with activated CD4+ T and only involves a small subset of iDCs. We analyzed whether this could be attributed to specific cellular restrictions during the viral life cycle. To study env-independent restriction infection, we used single-round assay pseudotyped vesicular stomatitis virus G protein (HIV-VSVG). Small interfering RNA–mediated depletion APOBEC3G/3F (A3G/3F), not TRIM5α, enhanced iDCs, indicating...
ABSTRACT In the early events of human immunodeficiency virus type 1 (HIV-1) infection, immature dendritic cells (DCs) expressing DC-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) receptor capture small amounts HIV-1 on mucosal surfaces and spread viral infection to CD4 + T in lymph nodes (22, 34, 45). RNA interference has emerged as a powerful tool gain insight into gene function. For this purpose, lentiviral vectors that express short hairpin (shRNA) for delivery...
Regulatory T cells (Tregs) play an important role in infections modulating host immune responses and avoiding overreactive immunity. The mechanisms underlying their action HIV-infected patients have not been well established. HIV can infect Treg, but little is known about the effects of infection on Treg phenotype function. objective this study was to investigate whether in-vitro modifies suppressive capacity cells.Because are a subset CD4 cells, could produce alterations methylation pattern...
For the last 20 years, idea of alternative prevention strategies based on use topical vaginally products to inhibit HIV-1 infection in women has been established. The concept a 'microbicide' product born out unavailability vaccine against and problems negotiating preventive prophylaxis by their partners, especially developing countries.We have developed evaluated polyanionic carbosilane dendrimers G3-S16 G2-NF16 with sulphated naphthylsulphonated end groups as nonspecific...
Abstract: Nanotechnology-derived platforms, such as dendrimers, are very attractive in several biological applications. In the case of human immunodeficiency virus (HIV) infection, polyanionic carbosilane dendrimers have shown great potential antiviral agents development novel microbicides to prevent sexual transmission HIV-1. this work, we studied mechanism two sulfated and naphthylsulfonated functionalized G3-S16 G2-NF16. They able inhibit viral infection at fusion thus entry step. Both...
Failure of anti-TNF therapy is a real concern in children with inflammatory bowel disease (IBD) owing to the limited therapeutic arsenal. Anti-TNF drugs modulate immune response, key driver chronic inflammation IBD. Accordingly, we analyzed changes frequency T-lymphocyte and cytokine levels after 6 weeks treatment identify potential biomarkers response drugs. We recruited 77 patients under 18 years age diagnosed IBD treated an drug. Using flow cytometry multiplex ELISA, 31 populations four...
SARS-CoV-2 has infected more than 200 million people worldwide, with 4 associated deaths. Although 80% of develop asymptomatic or mild COVID-19, can induce a profound dysregulation the immune system. Therefore, it is important to investigate whether clinically recovered individuals present sequelae. The potential presence long-term system could constitute risk factor for re-infection and development other pathologies. Here, we performed deep analysis in 35 COVID-19 previously compared 16...
According to estimates from the UNAIDS Global Report 2010 around 33.3 million adults were living with HIV/AIDS at end of 2009, majority them in developing countries. More than 50% are women. In world, more 90 percent all adolescent and adult HIV infections have resulted heterosexual intercourse (UNAIDS, http://www.unaids.org/data/epi2010/). The options for prevention HIV-infection abstinence or use condoms due lack effective vaccine therapy. Therefore, a good education preventive microbicide...
ABSTRACT The synthesis and characterization of four families anionic carbosilane dendrimers bearing carboxylate, phosphonate, naphthylsulfonate, sulfate terminal groups prepared by cycloaddition azide–alkyne catalyzed copper (CuAAC) are presented here. For the preparation these dendrimers, two strategies starting from azide‐terminated were followed: (i) click coupling neutral alkynes followed derivatization into moieties or (ii) alkynes. Both require different reaction conditions in order to...
Estradiol-based contraceptives and hormonal replacement therapy predispose women to Candida albicans infections. Moreover, during the ovulatory phase (high estradiol), neutrophil numbers decrease in vaginal lumen increase luteal progesterone). Vaginal secretions contain chemokines that drive migration into lumen. However, their expression ovarian cycle or response treatments are controversial role defense remains unknown. To investigate transepithelial of neutrophils, we used adoptive...
Abstract: Self-administered topical microbicides or oral preexposure prophylaxis could be very helpful tools for all risk groups to decrease the human immunodeficiency virus (HIV)-1 infection rates. Up until now, antiretrovirals (ARVs) have been most advanced microbicide candidates. Nevertheless, majority of clinical trials has failed in HIV-1 patients. Nanotechnology offers suitable approaches develop novel antiviral agents. Thereby, new nanosystems, such as carbosilane dendrimers, shown...
Abstract CD25, the alpha chain of IL-2 receptor, is expressed on activated effector T cells that mediate immune graft damage. Induction immunosuppression commonly used in solid organ transplantation and can include antibodies blocking CD25. However, regulatory (Tregs) also rely CD25 for their proliferation, survival, function. Therefore, CD25-blockade may compromise Treg protective role against rejection. We analysed vitro effect basiliximab (BXM) viability, phenotype, proliferation cytokine...
The development of mRNA vaccines represented a significant achievement in response to the global health crisis during SARS-CoV-2 pandemic. Evaluating vaccine efficacy entails identifying different anti-SARS-CoV-2 antibodies, such as total antibodies against Receptor Binding Domain (RBD) S-protein, or neutralizing (NAbs). This study utilized an innovative PETIA-based kit measure NAb, and investigation aimed assess whether levels anti-RBD IgG NAb uniformly measured 30 days after vaccination...
In the context of Covid-19 pandemic, fast development vaccines with efficacy around 95% preventing illness provides a unique opportunity to reduce mortality associated pandemic. However, in absence efficacious prophylactic medications and few treatments for this infection, induction robust protective immunity is required effective disease control, not only prevent but also infection shedding/transmission. The objective our study was analyze level specific humoral cellular T-cell responses...
Persistence of human immunodeficiency virus type 1 (HIV-1) constitutes a major obstacle in the control HIV-1 infection. Here we investigated whether CpG methylation promoter can directly influence expression genome and thereby contribute to persistence latency HIV-1. The levels were studied after bisulfite-induced modification DNA five Jurkat clonal cell lines transduced by an long terminal repeat (LTR)-driven retroviral vector expressing enhanced green fluorescent protein (GFP) primary...
Objective: Dendrimers have been shown as effective and safe anti-HIV compounds with great potential topical microbicides. Currently, the most advanced microbicides are based on antiretrovirals. However, nowadays none of them has any success in clinical trials. Design: Antiviral activity combinatorial profile different combinations between CCR5 co-receptor antagonist, maraviroc (MRV), polyanionic carbosilane dendrimers against HIV-1 strains were evaluated. Methods: Cellular in-vitro models...