A5059422275- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Multiple Sclerosis Research Studies
- Pluripotent Stem Cells Research
- Herpesvirus Infections and Treatments
- Acute Ischemic Stroke Management
- Cerebrovascular and Carotid Artery Diseases
- Mesenchymal stem cell research
- Nerve injury and regeneration
- Alzheimer's disease research and treatments
- Tryptophan and brain disorders
- Traumatic Brain Injury and Neurovascular Disturbances
- Neuroscience and Neuropharmacology Research
- Amyotrophic Lateral Sclerosis Research
- Intracranial Aneurysms: Treatment and Complications
- Peripheral Neuropathies and Disorders
- Autoimmune Neurological Disorders and Treatments
- Prion Diseases and Protein Misfolding
- Stress Responses and Cortisol
- Genetics and Neurodevelopmental Disorders
- Pharmacological Effects and Toxicity Studies
- Polyomavirus and related diseases
- Retinal Development and Disorders
- Virus-based gene therapy research
- Glaucoma and retinal disorders
Hadassah Medical Center
2016-2025
Hebrew University of Jerusalem
2013-2025
University Medical Center
2014-2022
University Hospital and Clinics
2022
Kitasato University
2020
Institució Catalana de Recerca i Estudis Avançats
2020
Centro de Investigación Biomédica en Red
2020
University of Pennsylvania
2020
Weizmann Institute of Science
2019
Hadassah Academic College
2000-2016
<h3>Objective</h3> To evaluate the feasibility, safety, and immunological effects of intrathecal intravenous administration autologous mesenchymal stem cells (MSCs) (also called<i>mesenchymal stromal cells</i>) in patients with multiple sclerosis (MS) amyotrophic lateral (ALS). <h3>Design</h3> A phase 1/2 open-safety clinical trial. <h3>Patients</h3> Fifteen MS (mean [SD] Expanded Disability Status Scale [EDSS] score, 6.7 [1.0]) 19 ALS Amyotrophic Lateral Sclerosis Functional Rating [ALSFRS]...
Human embryonic stem cells (hESCs) may potentially serve as a renewable source of for transplantation. In Parkinson's disease, hESC‐derived dopaminergic (DA) neurons replace the degenerated in brain. Here, we generated highly enriched cultures neural progenitors from hESCs and grafted into striatum Parkinsonian rats. The grafts survived at least 12 weeks, transplanted stopped proliferating, teratomas were not observed. differentiated vivo DA neurons, though low prevalence similar to that...
<h3>Objective</h3> To investigate the therapeutic potential of mesenchymal stromal cells (MSCs) in chronic model experimental autoimmune encephalomyelitis (EAE). <h3>Design</h3> Mesenchymal were obtained from bone marrow naïve C57BL and green fluorescent protein–transgenic mice cultured with Eagle minimum essential medium/alpha medium after removal adhering cells. Following 2 to 3 passages, MSCs injected intraventricularly or intravenously into which EAE had been induced myelin...
<h3>Importance</h3> Preclinical studies have shown that neurotrophic growth factors (NTFs) extend the survival of motor neurons in amyotrophic lateral sclerosis (ALS) and combined delivery these has a strong synergistic effect. We developed culture-based method for inducing mesenchymal stem cells (MSCs) to secrete factors. These MSC-NTF been be protective several animal models neurodegenerative diseases. <h3>Objective</h3> To determine safety possible clinical efficacy autologous...
Abstract Transplanted neural precursor cells remyelinate efficiently acutely demyelinated focal lesions. However, the clinical value of cell transplantation in a chronic, multifocal disease like multiple sclerosis will depend on ability transplanted to migrate foci brain. Here, we expanded newborn rat spheres and them intracerebroventricularly or intrathecally rats. The were labeled by nuclear fluorescent dye Hoechst incubation with BrdU enable their identification at 2 days weeks after...
Human embryonic stem (hES) cells are pluripotent derived from the inner cell mass of early preimplantation embryo. An efficient strategy for stable genetic modification hES may be highly valuable manipulating in vitro and promote study biology, human embryogenesis, development cell-based therapies. Here, we demonstrate that vectors self-inactivating (SIN) immunodeficiency virus type 1 (HIV-1) tools cells. Transduction by a modified vector SIN HIV-1 containing woodchuck hepatitis regulatory...
Abstract Objective Intracerebroventricular or intravenous (IV) injection of neural precursor cells (NPCs) attenuates experimental autoimmune encephalomyelitis (EAE), the animal model multiple sclerosis. Although stem cell therapy was introduced initially for replacement, we examine here whether NPCs possess immunomodulatory effects. Methods We examined effects systemic administration on central nervous system (CNS) inflammation in EAE and interactions between T vitro vivo. Results IV NPC...
Multiple sclerosis (MS) is an immune mediated demyelinating disease of the central nervous system (CNS). A potential new therapeutic approach for MS cell transplantation which may promote remyelination and suppress inflammatory process.We transplanted human embryonic stem cells (hESC)-derived early multipotent neural precursors (NPs) into brain ventricles mice induced with experimental autoimmune encephalomyelitis (EAE), animal model MS. We studied effect NPs on functional pathological...
Abstract Retinal and macular degenerations are a major cause of blindness. Cell transplantation is possible therapeutic approach for the replacement degenerating retinal cells. Here, we studied potential human embryonic stem cells (hESCs) to survive, integrate, differentiate into after intraocular transplantation. Highly enriched cultures neural precursors (NPs) expressing transcripts key regulatory genes development were developed from hESCs. After spontaneous differentiation in vitro, NPs...
Oligodendrocyte-type 2 astrocyte (O-2A) lineage cells are derived from multipotential stem of the developing CNS. Precursors O-2A progenitors express polysialylated (PSA) form neural cell adhesion molecule (NCAM) and detected in neonatal rat brain glial cultures. It is unclear how such PSA-NCAM+ "pre-progenitors" related to whether they still have potential differentiate along several lineages. Here we isolated pre-progenitor cultures by immunopanning found that most these expressed nestin...
Author(s): Tezel, Gulgun; Fourth ARVO/Pfizer Ophthalmics Research Institute Conference Working Group
Gliomas express many genes that play a role in neural precursor cells (NPCs), but no direct comparison between glioma and stem cell (SC) gene expression profiles has been performed. To investigate the similarities differences gliomas SCs, we compared microRNA (miRNA) signatures of glial tumors, embryonic SCs (ESCs), NPCs, normal adult brains from both human mouse tissues. We demonstrated (regardless their grade) methylcholanthrene-induced shared an miRNA profile is reminiscent NPCs. About...
To determine the frequency and significance of concurrent glial (glial-Ab) or neuronal-surface (NS-Ab) antibodies in patients with anti-NMDA receptor (NMDAR) encephalitis.Patients were identified during initial routine screening a cohort (C1) 646 consecutively diagnosed anti-NMDAR encephalitis another (C2) 200 systematically rescreened. Antibodies determined rat brain immunostaining cell-based assays.Concurrent 42 (4% from C1 7.5% C2): 30 (71%) glial-Ab 12 (29%) NS-Ab. Glial-Ab included...
Transplantation offers a means of identifying the differentiation and myelination potential early neural precursors, features relevant to myelin regeneration in demyelinating diseases. In postnatal rat brain, precursor cells expressing polysialylated (PSA) form cell adhesion molecule NCAM have been shown generate mostly oligodendrocytes astrocytes <i>in vitro</i> (Ben-Hur et al., 1998). Immunoselected PSA-NCAM+ newborn CNS precursors were expanded as clusters with FGF2 grafted into focal...
Abstract Stem cell transplantation is being explored as a new paradigm for the treatment of demyelinating diseases. Magnetically labeled multipotential neural precursor cells were transplanted into ventricles rats with acute experimental allergic encephalomyelitis (EAE) and high‐resolution (microscopic) MR images obtained ex vivo. Migration patterns live periventricular white matter structures could be easily visualized, good correlation corresponding histopathology. The present results...