A5060845930- Advanced Glycation End Products research
- Heart Rate Variability and Autonomic Control
- Birth, Development, and Health
- Pancreatic function and diabetes
- Chronic Kidney Disease and Diabetes
- Gestational Diabetes Research and Management
- MicroRNA in disease regulation
- Diabetes Treatment and Management
- Adipose Tissue and Metabolism
- Renal and related cancers
- Nitric Oxide and Endothelin Effects
- Cancer-related molecular mechanisms research
- Hormonal Regulation and Hypertension
- Diet, Metabolism, and Disease
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Diabetes Management and Research
- Sulfur Compounds in Biology
- Cardiovascular Syncope and Autonomic Disorders
- Cardiovascular Function and Risk Factors
- Diabetic Foot Ulcer Assessment and Management
- Coenzyme Q10 studies and effects
- Mitochondrial Function and Pathology
- Dialysis and Renal Disease Management
- Natural Antidiabetic Agents Studies
- Redox biology and oxidative stress
Universidade de São Paulo
2015-2024
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2015-2024
Centro Paulista de Investigação Clinica
2022
Universidade Federal de São Paulo
2022
Universidade Nove de Julho
2022
Aims: Thioredoxin interacting protein (TXNIP), an inhibitor of antioxidant thioredoxin (Trx), is upregulated by hyperglycemia and implicated in pathogenesis diabetes complications. We evaluated mRNA expressions genes encoding TXNIP Trx (TXN) urinary sediment peripheral blood mononuclear cells (PBMC) type 1 (T1D) patients with different degrees chronic Methods: qPCR was employed to quantify target (n = 55) PBMC 161) from sorted presence or absence diabetic nephropathy (DN), retinopathy,...
OBJECTIVE To compare the serum micro-RNAs (miRNAs) profile of individuals with type 1 diabetes without microvascular complications versus those multiple severe complications, in order to identify epigenetically modulated pathways these two groups individuals. RESEARCH DESIGN AND METHODS A total 10 subjects was selected among followed Diabetes Outpatient Clinic and sorted according absence or presence all complications. Samples from participants were used for evaluation miRNA expression...
To investigate epigenetic mechanisms potentially involved in the cognitive decline associated with chronic alcohol intake, we evaluated expressions of three micro-RNAs (miR-34a, -34b, and -34c) highly expressed hippocampus neuronal physiology pathology. MiR-34a participates functioning survival mature neurons; miR-34b is Alzheimer-like disorders; miR-34c implicated memory impairment Alzheimer disease rodents humans.A total 69 cases were selected from Biobank for Aging Studies categorized...
To assess the renal effects of chronic exposure to advanced glycation end-products (AGEs) in absence diabetes and potential impact concomitant treatment with antioxidant N-acetyl cysteine (NAC).Wistar rats received intraperitoneally 20 mg/kg/day albumin modified (AlbAGE) or not (AlbC) by for 12 weeks oral NAC (600mg/L; AlbAGE+NAC AlbC+NAC, respectively). Biochemical, urinary morphological analyses; carboxymethyl-lysine (CML, an AGE), CD68 (macrophage infiltration), 4-hydroxynonenal (4-HNE,...
Abstract Background Subclinical atherosclerosis is frequently observed in type 1 diabetes (T1D) although the mechanisms and markers involved evolution to established cardiovascular disease are not well known. High-density lipoprotein cholesterol T1D normal or even high, changes its functionality proteomics considered. Our aim was evaluate of HDL subfractions control subjects association with clinical variables, subclinical functionality. Methods A total 50 individuals 30 matched controls...
Quantitative polymerase chain reaction was employed to quantify expression of two genes coding for advanced glycation end-product receptors [RAGE ( AGER) and AGER1 DDOST)] the gene deacetylase SIRT1 SIRT1) in peripheral blood mononuclear cells from type 1 diabetes patients without [Group A, n = 35; 28.5 (24–39) years old; median (interquartile interval)] or with at least one microvascular complication B, 117; 34.5 (30–42) old]; 31 healthy controls were also included. In a subgroup 48...
Aim The pathogenesis of chronic diabetes complications has oxidative stress as one the major elements, and single-nucleotide polymorphisms (SNPs) in genes belonging to antioxidant pathways modulate susceptibility these complications. Considering that melatonin is a powerful compound, our aim was explore, longitudinal cohort study type 1 (T1D) individuals, association microvascular SNPs gene encoding receptor 1A ( MTNR1A ). Methods Eight were genotyped 489 T1D individuals. Besides...
Since hyperglycemia promotes inflammation by different pathways and participates in the development of chronic diabetes complications, we investigated association between leukotriene (LT) pathway microvascular complications.Quantitative polymerase chain reaction was employed to quantify expression ALOX5 (encodes 5-lipoxygenase), LTB4R one LTB4 receptors), MYD88 peripheral blood mononuclear cells from 164 type 1 (T1D) individuals presenting or not kidney disease, retinopathy, neuropathy,...
Introduction: Using a discovery/validation approach we investigated associations between panel of genes selected from transcriptomic study and the estimated glomerular filtration rate (eGFR) decline across time in cohort type 1 diabetes (T1D) patients. Experimental Urinary sediment was performed to select highly modulated T1D patients with rapid eGFR (decliners) versus stable (non-decliners). The were validated samples (n=54, mean duration 21 years, 61% women) followed longitudinally for...
11β-Hydroxysteroid dehydrogenase 1 has been implicated in insulin resistance (IR) the setting of metabolic disorders, and single nucleotide polymorphisms (SNPs) its encoding gene (HSD11B1) have associated with type 2 diabetes syndrome. In (T1D), IR related to development chronic complications. We investigated association HSD11B1 SNPs microvascular complications a Brazilian cohort T1D individuals.Five were genotyped 466 individuals (57% women; median 37 years old, duration 25 HbA1c 8.4%).The...
Abstract Aims/Introduction Epigenetics participate in the pathogenesis of metabolic memory, a situation which hyperglycemia exerts prolonged deleterious effects even after its normalization. We tested hypothesis that genetic variants an epigenetic gene could predispose to diabetes complications. Material and Methods assessed frequency five single‐nucleotide polymorphisms encoding deoxyribonucleic acid methytransferase 1 ( DNMT 1; rs8112895, rs7254567, rs11085721, rs17291414 rs10854076),...
The paucity of epidemiological data regarding diabetes complications in Brazil motivated us to evaluate the prevalence rates distal symmetric polyneuropathy (DSP) and cardiovascular autonomic neuropathy (CAN) individuals with type 2 (T2D) followed a primary care unit. A total 551 (59.3% women, 65 years old; duration 10 years; HbA1c 7.2%, medians) were included this cross-sectional study. DSP was diagnosed by sum Neuropathy Symptoms Score (NSS) Modified Disability (NDS) Semmes-Weinstein...
A low-grade inflammation is associated with cardiac autonomic neuropathy (CAN) and increased concentration of leukotriene B4 (LTB4) was found in individuals type 1 diabetes definitive CAN. This cross-sectional study evaluated plasma LTB4 other inflammatory mediators, namely, tumor necrosis factor (TNF), interleukin (IL)1B, IL10 2 (T2D) different degrees CAN, correlated these mediators the degree glycemic control a surrogate marker insulin resistance. TNF, IL1B, concentrations were measured...
Cardiovascular autonomic neuropathy (CAN) is a common complication of type 2 Diabetes mellitus (T2D), and prevalence varies according to the methodology used. CAN should be diagnosed in subclinical stage when an intensive treatment T2D could avoid progression irreversible phases.Determine early involvement (EI) individuals comparing two methodologies.This was cross-sectional study that included 183 who were monitored Tertiary centre. The diagnosis based on results four cardiovascular reflex...