Low-intensity pulsed ultrasound (LIPUS) has been used as an effective noninvasive method for treating fractures and osteoarthrosis, but the application in field of oral implantation is its infancy. This study aimed to clarify effect mechanism LIPUS on osteogenic differentiation bone marrow mesenchymal stem cells (BMSCs) implant osseointegration, provide experimental basis future clinical applications. Dental implants were inserted into Wistar rat femurs, was performed 4 weeks. Micro-CT...

The goal of this study was to explore new candidate genes and pathogenesis mechanisms nonsyndromic hereditary gingival fibromatosis (nsHGF) provide an experimental basis for the diagnosis nsHGF. Whole-exome sequencing (WES) performed on peripheral blood DNA from three nsHGF family members screen genes, Sanger related databases were used verify pathogenicity gene deficiency. Moreover, effects deficiency biological characteristics human fibroblasts (HGFs) evaluated via cell proliferation...

This study aimed to explore the effects of metformin on osteogenic differentiation alveolar bone marrow mesenchymal stem cells (BMSCs) from type-2 diabetes mellitus (T2DM) patients (DM-BMSCs) and implant osseointegration in rats, screen optimal concentration, investigate whether could protect against adverse impact T2DM BMSC capacity.Different concentrations were administered human-derived BMSCs Wistar rats receiving implants. ALP detection, alizarin red staining, real-time RT-PCR Western...

The success rate of oral implants is lower in type 2 diabetes mellitus (T2DM) patients than nondiabetic subjects; functional impairment bone marrow-derived mesenchymal stem cells (BMSCs) an important underlying cause. Many factors the blood can act on BMSCs to regulate their biological functions and influence implant osseointegration, but which play negative roles T2DM still unclear. This study aimed at screening differentially expressed genes from patients, identifying impact osteogenic...

AIM:To establish a novel mouse constipation model. METHODS:Animals were randomly divided into three groups, and intragastrically administered 0-4 ℃ saline (ice-cold group) or 15-20 (saline control daily for 14 d, left untreated (blank group).Stools collected 3-24 h after treatment to record the wet dry weights stool form.Intestinal propulsion experiments carried out defecation time was measured six days continuously suspending treatments.The expressions of PGP9.5 detected by...

Temporomandibular disorders are a common cause of chronic pain in the orofacial region and have complex multi-factorial pathophysiology. Mechanical loading or inflammatory conditions been shown to decrease oxygen tension within joint cartilage activate hypoxia-inducible factor (HIF) pathway, which turn aggravates pathological processes underlying temporomandibular (TMJ) disorders. We previously showed that low-intensity pulsed ultrasound (LIPUS) treatment effectively repairs TMJ injury...

LINC00472 is reported to play a role in suppressing tumors cancers such as lung cancer and hepatocellular carcinoma, among others. We made investigations into the effects of oral squamous cell carcinoma (OSCC) progression explore underlying molecular mechanisms. By qRT-PCR, we assessed expression OSCC tissues cells performed functional analysis investigate how LINC00472/miR-455-3p/ELF3 impacts proliferation, apoptosis, cycle. The that plays tumor growth was examined by establishing xenograft...

Chronic low-grade inflammation of visceral adipose tissue can cause obesity-associated insulin resistance, leading to metabolic syndrome. However, anti-inflammatory drugs and those for obesity management lead serious side effects such as abnormal heart rate blood pressure. Consequently, this study aimed explore the therapeutic potential electroacupuncture stimulation (ES) associated chronic inflammation. Sprague-Dawley male rats were fed a high-fat diet (HFD) ten weeks build an model, half...

Type 2 diabetes mellitus (T2DM) is a clinically important risk factor for dental implant treatment failure. An imbalance in the microenvironment of jawbone diabetic patients can impair functions bone marrow mesenchymal stem cells (BMSCs), thereby interfering with osseointegration during healing phase. This study aims to investigate potential molecular factors associated abnormal BMSC biological failure and identify intervention targets improve osseointegration. The results cell adhesion,...

The present study was designed to investigate the possible effect of chronic alcohol intake on propofol and remifentanil requirements, which determined by quantifying 50% (EC(50) ) 95% (EC(95) effective effect-site concentrations for at loss consciousness (LOC) after a painful stimulus.Thirty male patients (alcoholic group; n = 30) with alcoholism 30 (control history small were anaesthetized target-controlled infusion. predicted drug Bispectral Index (BIS) values recorded LOC no response...

Abstract Objective To determine whether risk factors other than hyperglycemia lead to failed osseointegration in patients with type 2 diabetes mellitus (T2DM) during the healing period. Materials and Methods We compared success rates between without T2DM period at our center. Bone marrow mesenchymal stem cells (BMSCs) were cultured from subjects. Proteomics was used detect differentially expressed proteins (DEPs) among DM failure (DM‐F), (DM‐S), control (Con) groups. The correlation...

Abstract Objectives To investigate the effect of liraglutide on osteogenesis in human alveolar bone marrow mesenchymal stem cells (BMSCs) and influence implant–bone integration rats with T2DM. Subjects methods Extracting BMSCs from alveoli diabetic patients treated insulin. were different concentrations liraglutide. Osteogenesis underlying mechanism investigated via ALP detection, staining, Alizarin Red S Western blotting, RT‐PCR. Liraglutide was given to Wistar GK after implantation, new...

Mandibular malpositioning may result in an abnormal concentration of stresses within the temporomandibular joint (TMJ) adult rats, which further lead to a series pathological changes, such as articular cartilage wear, subchondral bone sclerosis and osteophyte formation. However, adaptive changes condylar caused by different stress distributions are still controversial.

Background The incidence rate of adolescent depression and anxiety has been increasing since the outbreak COVID-19, which there are no effective therapeutic drugs available. Si-ni San is commonly used in traditional Chinese medicine for treatment depression-like as well anxiety-like behavior, but its mechanism treating combined with during adolescence not yet clear. Methods Network pharmacology was to explore potential drug molecules related targets, molecular docking dynamics (MD)...

Objective To compare the efficacy and safety of inpatient outpatient initiation ketogenic diet (KD) protocol pediatric refractory epilepsy. Methods Eligible children with epilepsy were randomly assigned to receive KD initiation. The generalized estimation equation (GEE) model was used analyze longitudinal variables seizure reduction, ketone body, weight, height, body mass index (BMI), BMI Z-score at different follow-up times between two groups. Results Between January 2013 December 2021, 78...

Low-intensity pulsed ultrasound (LIPUS) is an emerging physical therapy for the treatment of early temporomandibular joint injury and has a good effect on promoting cartilage subchondral bone tissue repair. However, best LIPUS intensity duration remain unclear. This study aimed at observing preventive therapeutic effects different modes identifying optimal regimen injury. In present study, rat models were established using chronic sleep deprivation (CSD) method, as intensities 30, 45, 60...

Background. Previous studies have demonstrated the efficacy of frequency-specific EAS. However, evaluation intensity-response effects is challenging and has yet to be addressed. Aims. Using cisapride promote gastric emptying, we measured relationship EA at ST25 on motility. Methods. We determined using intensities (0.5, 1, 3, 5, 7, 9 mA) motility in rats injected with (0.2, 0.02, 0.002 mg/kg). Results. Utilizing three concentrations yielded significantly differing levels Furthermore, log...

Temporomandibular joint osteoarthritis (TMJOA) is a degenerative disease with complex and multifactorial etiology. An increased intrajoint pressure or weakened penetration can exacerbate the hypoxic state of condylar cartilage microenvironment. Our group previously simulated environment TMJOA in vitro. Low-intensity pulsed ultrasound (LIPUS) stimulation attenuates chondrocyte matrix degradation via hypoxia-inducible factor (HIF) pathway-associated mechanism, but mode action LIPUS currently...

We aimed to find predictors for smartphone application-based ketogenic diet (KD) treatment effectiveness and safety.