A5055380735- Chronic Kidney Disease and Diabetes
- Dialysis and Renal Disease Management
- Renal Transplantation Outcomes and Treatments
- Renal Diseases and Glomerulopathies
- Organ Donation and Transplantation
- Birth, Development, and Health
- Renal and Vascular Pathologies
- Acute Kidney Injury Research
- Liver Disease Diagnosis and Treatment
- Organ Transplantation Techniques and Outcomes
- Diabetes Treatment and Management
- Electrolyte and hormonal disorders
- Ion Transport and Channel Regulation
- Renal function and acid-base balance
- Statistical Methods in Clinical Trials
- Genetic and Kidney Cyst Diseases
- Biosimilars and Bioanalytical Methods
- Liver Disease and Transplantation
- Biomedical Research and Pathophysiology
- Transplantation: Methods and Outcomes
- Erythropoietin and Anemia Treatment
- Blood Pressure and Hypertension Studies
- Radiomics and Machine Learning in Medical Imaging
- Heart Failure Treatment and Management
- Antibiotics Pharmacokinetics and Efficacy
Hospital Universitario de Canarias
2015-2025
Universidad de La Laguna
2014-2025
Hospital Universitario Fundación Jiménez Díaz
2019-2022
Universidad Autónoma de Madrid
2020-2022
Fundación Renal
2020
Federación Española de Enfermedades Raras
2020
Primary hyperoxaluria type 1 (PH1) is caused by deficient alanine-glyoxylate aminotransferase, the human peroxisomal enzyme that detoxifies glyoxylate. Glycolate one of best-known substrates leading to glyoxylate production, via glycolate oxidase (GO). Using genetically modified mice, we herein report GO as a safe and efficient target for substrate reduction therapy (SRT) in PH1. We first generated GO-deficient mouse (Hao1(-/-)) presented high urine levels but no additional phenotype. Next,...
Renal function can be estimated with formulas, which are inaccurate, or measured gold standard methods, reliable but unpractical. We propose to simplify the plasma clearance of iohexol, a method measure renal function, by dried blood spot (DBS) testing. compared glomerular filtration rate (GFR) values assessed DBS and reference analysis technique. tested in vitro agreement between non-volumetric volumetric Then, we performed clinical validation vivo 203 patients. The was evaluated...
Factors associated with renal functional reserve (RFR)-presence or absence-are not completely clear and remain underinvestigated. In this observational study, we evaluated the characteristics presence absence of RFR in healthy subjects-living kidney donors-before donation. Renal function was assessed measured glomerular filtration rate (GFR)-the clearance iohexol-and infusion amino acids. An increase GFR >10% after acid indicated RFR. We defined a priori three groups donors: without, using...
Chronic kidney disease (CKD) affects 10–13% of the population worldwide. CKD classification stratifies patients in five stages risk for progressive renal based on estimated glomerular filtration rate (eGFR) by formulas and albuminuria. However, reliability to reflect real function is a matter debate. The effect error unclear, particularly cystatin C–based equations. We evaluated large number C and/or creatinine-based definition 882 subjects with different clinical situations over wide range...
Formulas do not estimate renal function with acceptable precision and accuracy.We compared 51 creatinine-based and/or cystatin c-based formulas a gold standard (iohexol plasma clearance) in 193 transplant recipients using concordance correlation coefficient, total deviation index, coverage probability the error chronic kidney disease (CKD) stage classification.No formula showed coefficient greater than 0.90 (average for formulas: ∼0.70 ∼0.85). A wide index was observed: approximately 70%...
Bariatric surgery (BS) has been postulated as the most effective measure for weight reduction. Weight loss improves metabolic parameters and exerts changes in renal function that lead to amelioration of absolute or relative glomerular hyperfiltration, a condition may be renoprotective long term. However, few studies have demonstrated influence BS patients with severe obesity chronic kidney disease (CKD). Our objective was analyse evolution function, adipose tissue-derived molecules...
Serum and glucocorticoid-regulated kinase 1 (SGK1) stimulates aldosterone-dependent renal Na+ reabsorption modulates blood pressure. In addition, genetic ablation or pharmacological inhibition of SGK1 limits the development kidney inflammation fibrosis in response to excess mineralocorticoid signaling. this work, we tested hypothesis that a systemic increase activity would potentiate mineralocorticoid/salt-induced hypertension injury. To end, used transgenic mouse model with increased...
ABSTRACT Glomerulosclerosis and tubulointerstitial fibrosis are pathological features of chronic kidney disease. Transforming growth factor β (TGFβ) is a key player in the development fibrosis. However, three known TGFβ isoforms, only TGFβ1 has an established role fibrosis, pathophysiological relevance TGFβ2 TGFβ3 unknown. Because Tgfb3 deficiency mice results early postnatal lethality, we analyzed phenotype heterozygous Tgfb3-knockout (Tgfb3+/−) compared it with that matched wild-type mice....
There is an increasing demand for accurately measured glomerular filtration rate (GFR). Iohexol serum clearance has become a new gold standard, but it challenging when GFR low and 24-hour sampling required accurate results. The primary aim of this study was to develop iohexol pharmacokinetic population model determination individual using limited up 5 hours also renal function <40 ml/min.A nonparametric developed with rich data from 176 patients. In validation cohort 43 patients,...
Abstract Background Autosomal dominant polycystic kidney disease (ADPKD) causes about 10% of cases end stage renal disease. Disease progression rate is heterogeneous. Tolvaptan presently the only specific therapeutic option to slow function decline in adults at risk rapidly progressing ADPKD with chronic (CKD) stages 1–4. Thus, a reliable evaluation patients needed. Methods We evaluated agreement between measured (mGFR) and estimated glomerular filtration (eGFR) by 61 formulas based on...
An accurate assessment of renal function is needed in the majority clinical settings. Unfortunately, most used estimated glomerular filtration rate (eGFR) formulas are affected by significant errors comparison to gold standards methods measured GFR (mGFR).The objective study determine extent error eGFR compared mGFR different specific settings.A total retrospectively consecutive cohort 1,320 patients (pts) enrolled 2 European Hospitals (Center 1: 470 pts; Center 2: 850 pts) was collected...
Reliable determination of glomerular filtration rate (GFR) is crucial in the evaluation living kidney donors. Although some guidelines recommend use measured GFR (mGFR), many centres still rely on estimated (eGFR) obtained through equations or 24-h creatinine clearance. However, eGFR neither accurate nor precise reflecting real renal function. We analysed impact errors and decision making regarding potential evaluated 103 consecutive donors who underwent mGFR via iohexol plasma clearance by...
Type 2 diabetes mellitus represents 30-50% of the cases end stage renal disease worldwide. Thus, a correct evaluation function in patients with is crucial to prevent or ameliorate diabetes-associated kidney disease. The reliability formulas estimate still unclear, particular, those new equations based on cystatin-C combination creatinine and cystatin-C. We aimed assess error available glomerular filtration rate diabetic patients. evaluated and/or reflecting real over wide range (from...
There is no simple method to measure glomerular filtration rate (GFR) in swine, an established model for studying renal disease. We developed a protocol GFR conscious swine by using the plasma clearance of iohexol. used two groups, test and validation, with eight animals each. Ten milliliters iohexol (6.47 g) was injected into marginal auricular vein blood samples (3 mL) were collected from orbital sinus at different points after injection. determined models: two-compartment (CL2: all...