A5084837815- Wnt/β-catenin signaling in development and cancer
- Cancer-related gene regulation
- Genomic variations and chromosomal abnormalities
- Cancer Cells and Metastasis
- Bacteriophages and microbial interactions
- Chromosomal and Genetic Variations
- Kruppel-like factors research
- Genetic factors in colorectal cancer
- Genetics and Neurodevelopmental Disorders
- Microbial infections and disease research
- Prenatal Screening and Diagnostics
- Cell Adhesion Molecules Research
- Glycosylation and Glycoproteins Research
- Bioactive Compounds in Plants
- Neurogenetic and Muscular Disorders Research
- Galectins and Cancer Biology
- Gene expression and cancer classification
- Antifungal resistance and susceptibility
- Genetic Syndromes and Imprinting
- Congenital heart defects research
- Bacterial Identification and Susceptibility Testing
- Connective tissue disorders research
- Hippo pathway signaling and YAP/TAZ
- Molecular Biology Techniques and Applications
- Polyamine Metabolism and Applications
University College Ghent
2005-2024
Utrecht University
2011
Ghent University Hospital
1995-2005
Ghent University
1997-2005
Sanford Burnham Prebys Medical Discovery Institute
1998
Kyoto University
1998
Cancer Research Institute Ghent
1992
ABSTRACT The function of many transmembrane molecules can be altered by cleavage and subsequent release their ectodomains. We have investigated ectodomain the cell-cell adhesion signal-transducing molecule E- cadherin. E-cadherin is constitutively shed from surface MCF-7 MDCKts.srcC12 cells in culture. Release 80 kDa soluble fragment stimulated phorbol-12-myristate-13-acetate inhibited overexpression tissue inhibitor metalloproteinases-2. metalloproteinases matrilysin stromelysin-1 both...
αE-catenin, a cadherin-associated protein, is required for tight junction (TJ) organization, but its role poorly understood. We transfected an αE-catenin–deficient colon carcinoma line with series of αE-catenin mutant constructs. The results showed that the amino acid 326–509 domain this catenin was to organize TJs, and COOH-terminal not essential process. internal found bind vinculin. When NH2-terminal fragment, which by itself unable TJ, fused vinculin tail, chimeric molecule could induce...
Bacteriophages are a promising therapeutic strategy among cystic fibrosis and lung-transplanted patients, considering the high frequency of colonization/infection caused by pandrug-resistant bacteria. However, little clinical data available regarding use phages for infections with Achromobacter xylosoxidans. A 12-year-old patient received two rounds phage therapy because persistent lung infection A. Clinical tolerance was perfect, but initial bronchoalveolar lavage (BAL) still grew The...
In Belgium, the incorporation of phages into magistral preparations for human application has been permitted since 2018. The stability such is high importance to guarantee quality and efficacy throughout treatments. We evaluated ability preserve infectivity four different active against three bacterial species in five buffer infusion solutions commonly used medicine biotechnological manufacturing processes, at two concentrations (9 7 log pfu/mL), stored 4 °C. DPBS without Ca2+ Mg2+ was found...
Array CGH (comparative genomic hybridization) enables the identification of chromosomal copy number changes. The availability clone sets covering human genome opens possibility for widespread use array both research and diagnostic purposes. In this manuscript we report on parameters that were critical successful implementation technology, assess quality criteria, discuss potential benefits pitfalls technology improved pre- postnatal constitutional genetic diagnosis. We propose to name...
The α-catenin molecule links E-cadherin/ β-catenin or E-cadherin/plakoglobin complexes to the actin cytoskeleton. We studied several invasive human colon carcinoma cell lines lacking α-catenin. They showed a solitary and rounded morphotype that correlated with increased invasiveness. These round variants acquired more normal epithelial phenotype upon transfection an expression plasmid, but also treatment protein kinase C (PKC) activator 12-O-tetradecanoyl-phorbol-13-acetate (TPA). Video...
The availability of the human genome sequence as well large number physically accessible oligonucleotides, cDNA, and BAC clones across entire has triggered accelerated use several platforms for analysis DNA copy changes, amongst others microarray comparative genomic hybridization (arrayCGH). One challenges inherent to this new technology is management numbers data points generated in each individual experiment.
// Lynn Feys 1 , Benedicte Descamps 2 Christian Vanhove Anne Vral 3 Liv Veldeman 4 Stefan Vermeulen 5 Carlos De Wagter Marc Bracke and Olivier Wever Department of Radiation Oncology Experimental Cancer Research, Laboratory Ghent University, Ghent, Belgium Electronics Information System, iMinds-IBiTech-MEDISIP, Basic Medical Sciences, Physiology Group, Gent University Hospital, Biomedical Science, HoGent, Correspondence to: Wever, email: Keywords : radiotherapy, triple-negative breast cancer,...
The aim of the current study was to evaluate and compare two representative samples different classes adsorbents intended for use as feed additives in prevention or reduction adverse effects exerted by mycotoxins, specifically ochratoxin A (OTA) zearalenone (ZEN). adsorbents, an organically activated bentonite (OAB) a humic acid polymer (HAP), were tested common vitro model with pH course comparing maximum changes that can be expected digestive system monogastric animal, i.e. 7.4 oral...
RNA quality and quantity are important factors for ensuring the accuracy of gene expression analysis other RNA-based downstream applications. Thus far, only a limited number methodological studies have compared sample storage extraction procedures human cells. We three commercially available kits, i.e., (NucliSENS) easyMAG, RNeasy (Mini Kit) RiboPure (RNA Purification Kit–blood). In addition, additional conditions, such as medium temperature peripheral blood mononuclear cells were evaluated,...
The E-cadherin-catenin complex is important for the maintenance of epithelial architecture. We studied its expression in Crohn disease, ulcerative colitis, acute ileitis, and controls. Immunohistochemical stainings E-cadherin, alpha-catenin, beta-catenin gamma-catenin were performed. E-cadherin messenger RNA (mRNA) was detected using riboprobes. In active inflammation, there up-regulation complex. particular, epithelium adjacent to ulcers showed increased protein mRNA, but ulcer-associated...
Abstract To determine phage titers accurately, reproducibly and in a non-laborious cost-effective manner, we describe the development of qPCR platform for molecular quantification five phages present bacteriophage cocktail 2 (BFC2). We compared performance this approach, with regard to reproducibility, standard culture-based double agar overlay method (DAO). demonstrated that each BFC2 was possible by means qPCR, without prior DNA extraction, but yields were significantly higher comparison...
Abstract A moderately mentally retarded 10‐year‐old boy of very short stature was found initially to have a complex chromosomal rearrangement (CCR) involving chromosome 1, 2, 3, 4, and 8. balanced twelve‐breakpoint CCR suggested after extensive investigations including subtelomere FISH, whole paints, comparative genomic hybridization (CGH), multicolor FISH (MFISH), spectral karyotyping (SKY). SKY MFISH gave slightly discrepant results. For further clarification the karyotype, banding (MCB)...