A5077676537- Analytical Methods in Pharmaceuticals
- Antibiotics Pharmacokinetics and Efficacy
- Analytical Chemistry and Chromatography
- Hematopoietic Stem Cell Transplantation
- Analytical Chemistry and Sensors
- Mass Spectrometry Techniques and Applications
- Cardiac electrophysiology and arrhythmias
- Metabolomics and Mass Spectrometry Studies
- Transplantation: Methods and Outcomes
- Advanced Chemical Sensor Technologies
- Cardiac Imaging and Diagnostics
- Chemical Reactions and Isotopes
- Acute Myocardial Infarction Research
- Immune Cell Function and Interaction
- Amino Acid Enzymes and Metabolism
- Drug Transport and Resistance Mechanisms
- Cytomegalovirus and herpesvirus research
- Mesenchymal stem cell research
- Cardiac Ischemia and Reperfusion
- Hormonal and reproductive studies
- Metabolism and Genetic Disorders
- Adenosine and Purinergic Signaling
- Inflammasome and immune disorders
- Phosphodiesterase function and regulation
- Biochemical and biochemical processes
Sinai Hospital
2024
National Cancer Institute
2013-2023
National Institutes of Health
2013-2023
Center for Cancer Research
2023
Government of the United States of America
2021
Virginia Commonwealth University
1988-2017
Altria (United States)
2010
Virginia Commonwealth University Medical Center
1988-2008
Parenterally administered ascorbic acid modulates sepsis-induced inflammation and coagulation in experimental animal models. The objective of this randomized, double-blind, placebo-controlled, phase I trial was to determine the safety intravenously infused patients with severe sepsis. Twenty-four sepsis medical intensive care unit were randomized 1:1:1 receive intravenous infusions every six hours for four days acid: Lo-AscA (50 mg/kg/24 h, n = 8), or Hi-AscA (200 Placebo (5% dextrose/water,...
Abstract The use of bone marrow-derived mesenchymal stromal cells (BMSC) in the treatment alloimmune and autoimmune conditions has generated much interest, yet an understanding therapeutic mechanism remains elusive. We therefore explored immune modulation by a clinical-grade BMSC product model human-into-mouse xenogeneic graft-versus-host disease (x-GVHD) mediated human CD4+ Th1 cells. reversed established, lethal x-GVHD through marked inhibition cell effector function. Gene marking studies...
Abstract Graft-versus-host disease (GVHD) is a prominent barrier to allogeneic hematopoietic stem cell transplantation (AHSCT). Definitive diagnosis of GVHD invasive, and biopsies involved tissues pose high risk bleeding infection. T cells are central pathogenesis, our previous studies in chronic mouse model showed that alloreactive CD4+ traffic the target organs ahead overt symptoms. Because increased glycolysis an early feature T-cell activation, we hypothesized vivo metabolic imaging...
Bronchiolitis obliterans syndrome (BOS) is a severe manifestation of chronic graft-versus-host disease (cGVHD) following hematopoietic cell transplantation (HCT). Montelukast interrupts cysteinyl leukotriene (CysLT) activity and may diminish the activation homing cells to bronchioles subsequent fibrosis. We performed prospective phase II trial test whether montelukast altered lung decline for patients with BOS after HCT. In this single-arm, open-label, multi-institutional study, primary...
Abstract A rapid and simple chemiluminescence method was developed for detection of inosine hypoxanthine in human plasma. The utilized a microplate luminometer with direct injectors to automatically dispense reagents during sample analysis. Enzymatic conversions hypoxanthine, followed by xanthine uric acid, generated superoxide anion radicals as useful metabolic by‐product. free react Pholasin ® , sensitive photoprotein used detection, produce measurable blue‐green light. use signal...
Deuterated water (2H2O) is a label commonly used for safe quantitative measurement of deuterium enrichment into DNA proliferating cells. More recently, it has been labeling proteins and other biomolecules. Our in vitro - vivo research reports important stable isotopic differences the nucleosides their isotopologues (e.g. deoxyadenosine (dA) M + 1, dA 2, 3), as well tumor cell proliferation effects various forms commercially available heavy (2H2O, H218O, 2H218O). Using an mouse thymus line,...
To assess the influence of in vitro and vivo hemodialysis with a new high-flux dialyzer on clearance cefazolin cefepime; to correlation dialytic these antibiotics blood flow rate; between clearances antibiotics.Prospective, open-label, dialysis study.A tertiary-care, university health science center.Five adults who received 3 times/week. Intervention. For experiment, patients single intravenous infusion 1 g cefepime before then underwent modified session. buffered simulated plasma water...
A rapid and sensitive gas chromatography-tandem mass spectrometry (GC-MS/MS) method was developed to quantitatively measure low levels of DNA base deoxyadenosine (dA) its isotopologues (e.g., dA M+1) from limited mouse cell populations. Mice undergoing allogeneic hematopoietic transplantation (AHSCT) received deuterated water at biologically relevant time intervals post AHSCT, allowing labeling upon division, which detected as the M+1 isotopologue. Targeted populations were isolated lymphoid...
In vivo deuterated water (2H2O) labeling leads to deuterium (2H) incorporation into biomolecules of proliferating cells and provides the basis for its use in cell kinetics research. We hypothesized that rapidly cancer would become preferentially labeled with 2H and, therefore, could be visualized by magnetic resonance imaging (dMRI) following a brief period systemic 2H2O administration. initiated administration two xenograft mouse models harboring either human colorectal, HT-29, or...